Abstract
The need for conservation of biotic diversity is well recognized. However, improved techniques for the efficient, cost effective-preservation of plant germplasm are needed. The conservation and distribution of plant germplasm in vitro is gaining acceptance. However, increased usage is dependent upon the ability of curators to minimize culture maintenance requirements. This report examines the effect of various levels of sucrose, photoperiod, temperature, sorbitol and mannitol on minimal growth storage of Ipomoea batatas (L.) Lam. Growth was reduced 50% with a temperature reduction of from 21.1 to 15.6°C. Sucrose concentrations of 15 and 20 g l-1 resulted in reduced plant stature with few adverse effects on plantlet viability or morphology. Reduction of photoperiod from 16 to 4 h produced smaller, slightly chlorotic, but otherwise normal plants. The addition of sorbitol or mannitol to culture media generally produced undesirable effects on gross plant morphology and loss of apical dominance. Genotype x growth retarding treatment interactions were observed for all variables examined.
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Abbreviations
- PL:
-
plant introduction
- f.w.:
-
fresh weight
- SE:
-
standard error
References
Banerjee N & De Langhe E (1985) A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and plantain). Plant Cell Rpts. 4: 351–354
Barlass M & Skene KGM (1983) Long-term storage of grape in vitro. Plant Genet. Res. Newsl. 53: 19–21
Chavez R, Roca WM & Williams JT (1987) IBPGR-CIAT collaborative project on a pilot in vitro active genebank. Plant. Genet. Res. Newsl. 71: 11–13
Christensen E (1987) Genetic ark: A proposal to preserve genetic diversity for future generations. Stanford Law Rev. 40: 279–321
Dodds, JH (1987) Review of in vitro propagation and maintenance of sweetpotato germplasm. In: Gregory, P (Ed) Exploration, Maintenance and Utilization of Sweet Potato Genetic Resources (pp 323–330). Rpt. 1st Plan Conf, Intl Potato Center, Lima, Peru
Henshaw GG, O'Hara JF & Westcott RJ (1980) Tissue culture methods for the storage and utilization of potato germplasm. In: Ingram DS & Helgeson JP (Eds) Tissue Culture Methods for Plant Pathologists, (pp 71–76). Blackwell Scientific, Oxford
Henshaw GG & O'Hara JF (1983) In vitro approaches to the conservation and utilization of global plant genetic resources. In: Mantell SH & Smith H (Eds) Plant Biotechnology (pp 219–238). Cambridge University Press, Cambridge
IBPGR (1987) Meristem-tip culture and virus indexing of sweet potstoes (pp 4–6). IBPGR, Rome
Jarret RL (1989) A repository for sweetpotato germplasm. HortScience 28: 836
Jarret RL & Florkowski W (1990) In vitro active vs field gene bank maintenance of sweetpotato germplasm: Major costs and other considerations. HortScience 25: 141–146
Jarret RL & Gawel N (1991) Abscissic acid-induced growth inhibition of sweetpotato (Ipomoea batatas L.) in vitro. Plant Cell Tiss. Org. Cult. 24: 13–18
Mix G (1982) In vitro preservation of potato material. Plant Genet. Res. Newsl. 51: 6–8
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Schnapp SR & Preece JE (1986) In vitro growth retardation of tomato and carnation microplants. Plant Cell Tiss. Org. Cult. 6: 3–8
Shands HR & Sisson VA (1989) Plant germplasm maintenance: An example of a national program. In: Stalker HT & Chapman C (Eds) Scientific Management of Germplasm: Characterization, Evaluation and Enhancement (pp 7–16). IBPGR, Rome, Italy
Towill LE (1988) Genetic considerations for germplasm preservation of clonal materials. HortScience 23: 91–97
Withers LA & Williams JT (1986) In vitro conservation. International Board for Plant Genetic Resources Research Highlights 1984–85, IBPGR Rome, Italy
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Jarret, R.L., Gawel, N. Chemical and environmental growth regulation of sweetpotato (Ipomoea batatas (L.) Lam.) in vitro. Plant Cell Tiss Organ Cult 25, 153–159 (1991). https://doi.org/10.1007/BF00042187
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DOI: https://doi.org/10.1007/BF00042187