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Cryopreservation of shoot tips of Caryophyllaceae ornamentals

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Summary

Shoot tips of 5 genera 38 species and cultivars of the Caryophyllaceae ornamentals were cryopreserved. Excised shoot tips were cooled at a rate of 0.5°C/min down to -40°C in the presence of 10% dimethyl sulphoxide and 3% glucose prior to immersion into liquid nitrogen. The shoot tips of all species and cultivars were viable after thawing. Little variation among species was observed in shoot regeneration. High viability of the cryopreserved shoot tips was maintained for up to 4 years. The plants derived from the cryopreserved shoot tips grew and flowered normally.

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Abbreviations

BA:

6-benzylaminopurine

DMSO:

dimethyl sulphoxide

MS:

Murashige & Skoog

NAA:

1-naphtalenacetic acid

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Authors and Affiliations

  1. Faculty of Agriculture, Kagawa University, Miki-cho, 761-07, Kita-gun, Kagawa, Japan

    Seiichi Fukai, Masanori Goi & Michio Tanaka

Authors
  1. Seiichi Fukai
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  2. Masanori Goi
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  3. Michio Tanaka
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Fukai, S., Goi, M. & Tanaka, M. Cryopreservation of shoot tips of Caryophyllaceae ornamentals. Euphytica 56, 149–153 (1991). https://doi.org/10.1007/BF00042058

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  • DOI: https://doi.org/10.1007/BF00042058

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