Summary
Nine japonica × indica F1 hybrids of rice involving 6 indica and 3 japonica tropical varieties, were large scale anther cultured. The frequency of callusing anthers averaged 18.7%. The microspore-derived calli produced green plants with a mean frequency of 8.7%. Albino plants represented 61% of the shoot forming calli. Monitoring of the green and albino plant regenerating capabilities of calli arising between week 4 and week 8 of incubation of the anthers showed no increase of the albino/green ratio and a slow decrease of the shoot forming ability of the transferred calli after the sixth week of culture. Spontaneous doubled haploids (SDH) represented 46% of the regenerated green plants in 4 hybrids. However, a high frequency of partially sterile regenerants was noticed among 132 SDH plants generated from a hybrid.
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Guiderdoni, E., Galinato, E., Luistro, J. et al. Anther culture of tropical japonica × indica hybrids of rice (Oryza sativa L.). Euphytica 62, 219–224 (1992). https://doi.org/10.1007/BF00041756
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DOI: https://doi.org/10.1007/BF00041756