A comparative study on anther development in male-fertile and male-sterile plants of Pelargonium crispumL'Her. ex Ait.

Summary

Anther development of male-fertile and male-sterile plants in Pelargonium crispum was anatomically examined. Three cultivars, i.e., ‘Lemon crispum’, ‘Crispum minor’ and ‘Prince Rupert’, were used. ‘Lemon crispum’ and ‘Crispum minor’ are male-fertile, whereas ‘Prince Rupert’ is male-sterile. The tapetum in every cultivar examined behaved like an amoeba. The tapetal cells of the anther form plasmodial masses. Then, the plasmodial masses fuse producing a periplasmodium. The periplasmodium degenerates and finally disappears. There are no differences in tapetal behaviour between fertile and sterile anthers. In the sterile anthers the endothecium and lip cells do not develop sufficiently. Young microspores show normal growth at early stages. After the completion of their cell wall formation, however, the microspores in the sterile anthers lose their cytoplasm and become empty. On the other hand, the microspores in the fertile anthers increase the volume of their cytoplasm and become fertile pollen grains.