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Hybridization between diploid (Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton through ovule culture

  • Interspecific Crosses
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Summary

With in vitro culture of ovules, interspecific hybrids have been obtained in an otherwise incompatible cross between a diploid (Gossypium arboreum) and a tetraploid (G. hirsutum) cultivated cotton. The early abortion of the embryo was prevented by repeated treatment of the flowers, immediately after pollination with a solution of gibberellic acid and naphthalene acetic acid. The ovules excised three days after pollination and cultured in a liquid medium underwent profuse proliferation, whereas on an agar-solidified medium supplemented with casein hydrolysate, indoleacetic acid and kinetin they germinated to form hybrid plants.

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References

  • Bajaj, Y. P. S. (Ed.), 1986. Biotechnology in agriculture and forestry 2. Crops. I, Springer Verlag, Berlin/ Heidelberg/New York/Tokyo. 608 pp.

    Google Scholar 

  • Bajaj, Y. P. S. & Manjeet S. Gill, 1985. In vitro induction of genetic variability in cotton (Gossypium spp.). Theor. Appl. Genet. 70: 363–368.

    Google Scholar 

  • Gill, M. S. & Y. P. S. Bajaj, 1984. Interspecific hybridization in the genus Gossypium through embryo culture. Euphytica 33: 305–311.

    Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15: 473–497.

    Google Scholar 

  • Pundir, N. S., 1972. Experimental embryology of Gossypium arboreum L. and Gossypium hirsutum, and their reciprocal crosses. Bot. Gaz. 133: 7–26.

    Google Scholar 

  • Stewart, J. M. & C. L. Hsu, 1978. Hybridization of diploid and tetraploid cottons through in-ovulo embryo culture. J. Heredity 69: 404–408.

    Google Scholar 

  • Weaver, J. B.Jr. 1957. Embryological studies following interspecific crosses'in Gossypium L. G. hirsutum × G. arboreum. Amer. J. Bot. 44: 209–214.

    Google Scholar 

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Authors and Affiliations

  1. Punjab Agricultural University, 141004, Ludhiana, India

    Manjeet S. Gill & Y. P. S. Bajaj

Authors
  1. Manjeet S. Gill
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  2. Y. P. S. Bajaj
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Gill, M.S., Bajaj, Y.P.S. Hybridization between diploid (Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton through ovule culture. Euphytica 36, 625–630 (1987). https://doi.org/10.1007/BF00041512

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  • DOI: https://doi.org/10.1007/BF00041512

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