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Nucleotide sequence analysis of a novel globulin1 null allele from the Illinois high protein strain of maize

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Abstract

The highly polymorphic maize globulin1 (glbl) gene encodes an abundant embryo storage protein. The present study extends the analysis of glbl variants to further explore the nature of polymorphism at this locus. The null allele Glb1-N1Hb, derived from the Illinois High Protein (IHP) strain of maize was characterized at the molecular level by nucleotide sequence analysis. Among other differences, a single-base insertion leading to a premature termination codon in the carboxyl-terminal half of the otherwise normal protein was observed. The likely reasons for the absence of GLB1 protein accumulation in the IHP strain of maize are discussed.

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References

  1. Belanger FC, Kriz AL: Molecular characterization of the major maize embryo globulin encoded by the Glb1 gene. Plant Physiol 91: 636–643 (1989).

    Google Scholar 

  2. Belanger FC, Kriz AL: Molecular basis for allelic polymorphism of the maize Globulin-1 gene. Genetics 129: 863–872 (1991).

    Google Scholar 

  3. Bhattramakki D, Sachs MM, Kriz AL: Expression of genes encoding globulin and prolamin storage proteins in kernels of Illinois long term chemical selection strains. Crop Sci 36: 1029–1036.

  4. Breathnach R, Chambon P: Organization and expression of eukaryotic split genes coding for proteins. Annu Rev Biochem 50: 349–383 (1981).

    Google Scholar 

  5. Brown JWS: A catalogue of splice junction and putative branch point sequences from plant introns. Nucl Acids Res 14: 9549–9559 (1986).

    Google Scholar 

  6. Hong B, Uknes SJ, Ho T-HD: Cloning and characterization of cDNA encoding a mRNA rapidly induced by ABA in barley aleurone layers. Plant Mol Biol 11: 495–506 (1988).

    Google Scholar 

  7. Jofuku KD, Schipper RD, Goldberg RB: A frameshift mutation prevents Kunitz trypsin inhibitor mRNA accumulation in soybean embryos. Plant Cell 1: 427–435 (1989).

    Google Scholar 

  8. Kriz AL: Characterization of embryo globulins encoded by the maize glb genes. Biochem Genet 27: 239–251 (1989).

    Google Scholar 

  9. Liu C-N, Rubenstein I: Transcriptional characterization of an α-zein gene cluster in maize. Plant Mol Biol 22: 323–336 (1993).

    Google Scholar 

  10. Mundy J, Rogers JC: Selective expression of a probable amylase protease inhibitor in barley aleurone cells: a comparison to the barley amylase/subtilisin inhibitor. Planta 169: 51–63 (1986).

    Google Scholar 

  11. Mount SM: A catalogue of splice junction sequences. Nucl Acids Res 10: 459–472 (1982).

    Google Scholar 

  12. Osterman JC: An allele of the Prot locus in maize is a variant for the site of protein processing. Biochem Genet 26: 463–474 (1988).

    Google Scholar 

  13. Rogers JC, Millman C: Isolation and sequence analysis of a barley α-amylase cDNA clone. J Biol Chem 258: 8169–8174 (1983).

    Google Scholar 

  14. Sanger FS, Nicklen S, Coulsen AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    Google Scholar 

  15. Scallon RJ, Dickinson CD, Nielsen NC: Characterization of a null-allele of the Gy4 glycinin gene from soybean. Mol Gen Genet 208: 107–113 (1987).

    Google Scholar 

  16. Schwartz D: Analysis of the size alleles of the Pro gene in maize: evidence for a mutant protein processor. Mol Gen Genet 174: 233–240 (1979).

    Google Scholar 

  17. Voelker TA, Moreno J, Chrispeels MJ: Expression analysis of a pseudogene in transgenic tobacco: a frameshift mutation prevents mRNA accumulation. Plant Cell 2: 255–261 (1990).

    Google Scholar 

  18. Voelker TA, Staswick P, Chrispeels MJ: Molecular analysis of two phytohemagglutinin genes and their expression in Phaseolus vulagaris cv. Pinto, a lectin-deficient cultivar of the bean. EMBO J 5: 3075–3082 (1986).

    Google Scholar 

  19. Yanisch-Perron C, Vieira J, Messing J: Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103–119 (1985).

    Google Scholar 

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Bhattramakki, D., Kriz, A.L. Nucleotide sequence analysis of a novel globulin1 null allele from the Illinois high protein strain of maize. Plant Mol Biol 32, 1215–1219 (1996). https://doi.org/10.1007/BF00041409

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  • DOI: https://doi.org/10.1007/BF00041409

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