Abstract
We present here the characterization of a Lupinus albus β-tubulin gene, TubB2, which is closely related to TubB1 [22]. Both TubB1 and TubB2 transcripts are present in the embryonic axis of lupin dry seeds. The patterns of developmental expression of TubB1 and TubB2 β-tubulin genes are strongly correlated. Both genes are expressed at higher levels in hypocotyls of 7-day-old etiolated plants compared to hypocotyls from plants grown under light/dark cycles. When etiolated plants are exposed to continuous white light, differential changes in the steady state levels of the TubB1 and TubB2 mRNAs from hypocotyls are observed. These changes are accompanied by an inhibition of hypocotyl extension.
This is a preview of subscription content, log in via an institution to check access.
References
Bewely JD, Black M: Seeds Physiology of Development and Germination. Plenum Press, New York (1985).
Bhat SP, Padayatty JD: Transcriptional events during early phase of germination of rice embryos. Nature 256: 227–228 (1975).
Bustos MM, Guiltinan MJ, Cyr RJ, Ahdoot D, Fosket DE: Light regulation of β-tubulin gene expression during internode development in soybean (Glycine max [L.] Merr.). Plant Physiol 91: 1157–1161 (1989).
Colbert JT, Costigan SA, Zhao Z: Photoregulation of β-tubulin mRNA abundance in etiolated oat and barley seedlings. Plant Physiol 93: 1196–1202 (1990).
Creelman RA, Mullet JE: Water deficit modulates gene expression in growing zones of soybean seedlings. Analysis of differentially expressed cDNAs, a new β-tubulin gene, and expression of genes encoding cell wall proteins. Plant Mol Biol 17: 591–608 (1991).
Cuming AC, Lane BG: Protein synthesis in imbibing wheat embryos. Eur J Biochem 99: 217–224 (1979).
Cyr RJ, Bustus MM, Guiltinan MJ, Fosket DE: Developmental modulation of tubulin protein and mRNA levels during somatic embriogenesis in cultured carrot cells. Planta 171: 365–376 (1987).
Cyr RJ, Palevitz BA: Organization of cortical microtubules in plant cells. Curr Opin Cell Biol 7: 65–71 (1995).
De Castro RD, Zheng X, Bergervoet JHW, De Vos CHR, Bino RJ: β-Tubulin accumulation and DNA replication in imbibing tomato seeds. Plan Physiol 109: 499–504 (1995).
Guiltinan MJ, Ma D-P, Barker RF, Bustos MM, Cyr RJ, Yadegari R, Fosket DE: The isolation, characterization and sequence of two divergent β-tubulin genes from soybean Plant Mol Biol 16: 225–234 (1987).
Harris B, Dure L: Developmental regulation in cotton seed germination: polyadenylation of stored messenger RNA. Biochemistry 17: 3250–3256 (1978).
Hussey PJ, Haas N, Hunsperger J, Larkin J, Snustad DP, Silflow CD: The β-tubulin gene family in Zea mays: two differentially expressed β-tubulin genes. Plant Mol Biol 15: 957–972 (1990).
Kang MS, Choi YJ, Kim MC, Lim CO, Hwang I, Cho MJ: Isolation and characterization of two β-tubulin cDNA clones from rice. Plant Mol Biol 26: 1975–1979 (1994).
Leu W-M, Cao X-L, Wilson TJ, Snustad DP, Chua N-H: Phytochrome A and phytochrome B mediate the hypocotyl-specific downregulation of TUB1 by light in Arabidopsis. Plant Cell 7: 2187–2196 (1995).
Liaud M-F, Binkmann H, Cerff R: The β-tubulin gene family of pea: primary structures, genomic organization and introndependent evolution of genes. Plant Mol Biol 18: 639–651 (1992).
Marks MD, West J, Weeks DP: The relatively large beta-tubulin family of Arabidopsis thaliana contains a member with an unusual transcribed 5′ noncoding sequence. Plant Mol Biol 10: 91–104 (1987).
Ortunño A, Sánches-Bravo J, Acosta M, Sabater F: Evolution and distribution of growth in etiolated hypocotyls of Lupinus albus. Biol Plant 30: 268–273 (1988).
Snustad DP, Haas NA, Kopczak SD, Silflow CD: The small genome of Arabidopsis contains at least nine expressed β-tubulin genes. Plant Cell 4: 549–556 (1992).
Spiegel S, Marcus A: Polyribosome formation in early wheat embryo germination independent of either transcription or polyadenylation. Nature 256: 228–230 (1975).
Staiger CJ, Lloyd CW: The plant cytoskeleton. Curr Opin Cell Biol 3: 33–42 (1991).
Takahashi T, Gasch A, Nishizawa N, Chua NH: The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation. Genes Devel 9: 97–107 (1995).
Vassilevskaia TD, Ricardo CP, Rodrigues-Pousada C: Molecular cloning and sequencing analysis of a β-tubulin gene from Lupinus albus. Plant Mol Biol 22: 715–718 (1993).
Villemur R, Haas NA, Joyce CM, Snustad DP, Silflow CD: Characterization of four new β-tubulin genes and their expression during male flower development in maize (Zea mays L.). Plant Mol Biol 24: 295–315 (1994).
Weeks D, Silflow C, Snustad P, Fosket D: Genes encoding tubulins. Plant Mol Biol Rep 12: S76 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vassilevskaia, T.D., Bekman, E., Jackson, P. et al. Developmental expression and regulation by light of two closely related β-tubulin genes in Lupinus albus . Plant Mol Biol 32, 1185–1189 (1996). https://doi.org/10.1007/BF00041404
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00041404