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Factors affecting in vitro propagation of Yucca glauca


A micropropagation system was developed to facilitate the release and subsequent testing of unique pink- or white-flowered selections of Yucca glauca. Shoot tip explants from mature plants were cultured on Murashige and Skoog medium supplemented with factorial combinations of α-naphthaleneacetic acid (NAA) (0.0 to 3.2 μM) and 6-benzylaminopurine (BA) (0.0 to 45 μM). Shoots were found to proliferate with increasing concentrations of BA and to produce callus and poorer quality shoots in the presence of NAA and the absence of BA. The response to BA and NAA was similar among 3 genotypes examined. A comparison of BA and N6-(Δ2-isopentenyladenine) (2iP) showed that 2iP was not effective in promoting shoot proliferation. Shoot tips rooted in the absence of growth regulators or in the presence of low concentrations of indole-3-butyric acid (IBA). Plantlets were successfully acclimated to greenhouse conditions.

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  1. Alexander RR, Pond FWA (1974) Yucca L. In: Seeds of Woody Plants in the United States, pp 857–858. USDA, Agric. Handbook 450, Washington, DC

    Google Scholar 

  2. D'Amato F (1978) Chromosome number variation in cultured cells and regenerated plants. In: Thorpe TA (Ed) Frontiers of Plant Tissue Culture, pp 287–295. Proc 4th Int Congr Plant Tissue Culture, Calgary

  3. Egolf DR (1976) The National Arboretum introduction program for new and improved shrubs and trees. In: Better Trees for Metropolitan Landscapes: Symposium Proceedings, pp 245–252. USDA Forest Serv Gen Tech Rep NE22

  4. Evans DA, Sharp WR, Medina-Filho HP (1984) Somaclonal and gametoclonal variation. Amer J Bot 71: 759–774

    Google Scholar 

  5. Fienberg SE (1978) The Analysis of Cross-Classified Categorical Data. MIT Press, Cambridge, MA

    Google Scholar 

  6. Fridborg G (1971) Growth and organogenesis in tissue cultures of Allium cepa var. proliferum. Physiol Plant 25: 436–440

    Google Scholar 

  7. Letham DS, Palni LMS (1983) The biosynthesis and metabolism of cytokinins. Ann Rev Plant Physiol 34: 163–197

    Google Scholar 

  8. Litz RE, Conover RA (1977) Tissue culture propagation of some foliage plants. Proc Fla State hort Soc 90: 301–303

    Google Scholar 

  9. Mok CM, Mok DWS, Dixon SC, Armstrong DJ, Shaw G (1982) Cytokinin structure-activity relationships and the metabolism of N6-(Δ2-isopentenyladenine)adenosine-8-14C in Phaseolus callus tissues. Plant Physiol 70: 173–178

    Google Scholar 

  10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  11. Norton ME, Norton CR (1985) In vitro propagation of Ericaceae: A comparison of the activity of the cytokinins n6-benzyladenine and n6-isopentenyladenine in shoot proliferation. Scientia Hort 27: 335–340

    Google Scholar 

  12. Pierik RLM, Steegmans HHM (1983) Vegetative propagation of a chimerical Yucca elephantipes Regel in vitro. Scientia Hort 21: 261–272

    Google Scholar 

  13. Robert ML, Herrera JL, Contreras F, Scorer KN (1987) In vitro propagation of Agave fourcroydes (Henequen). Plant Cell Tissue Organ Culture 8: 37–48

    Google Scholar 

  14. Skirvin RM (1978) Natural and induced variation in tissue culture. Euphytica 27: 241–266

    Google Scholar 

  15. Sokal RR, Rohlf FJ (1981) Biometry — the principles and practices of statistics in biological research. W. H. Freeman and Co., San Francisco, CA

    Google Scholar 

  16. Whitty CD, Hall RH (1974) A cytokinin oxidase in Zea mays. Can J Biochem 52: 789–799

    Google Scholar 

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Bentz, S.E., Parliman, B.J., Talbott, HJ. et al. Factors affecting in vitro propagation of Yucca glauca . Plant Cell Tiss Organ Cult 14, 111–120 (1988).

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Key words

  • Micropropagation
  • monocot
  • Liliaceae