Abstract
The pump-probe kinetics of the slowest spectral equilibrations between inequivalent BChl a Qy states in FMO trimers from Chlorobium tepidum are decelerated by nearly two orders of magnitude when the temperature is lowered from 300 K to 19 K. The pump-probe anisotropy decays are also markedly slower at 19 K than at 300 K. Singlet-singlet annihilation in FMO trimers is negligible at the laser powers used here. However, reduced temperatures greatly accentuate the probability of singlet-triplet annihilation, due to accumulation of metastable BChl a states under high laser repetition rates.
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Abbreviations
- BChl:
-
bacteriochlorophyll
- FMO:
-
Fenna-Matthews-Olson
- fwhm:
-
full width at half maximum
- PB:
-
photobleaching
- SE:
-
stimulated emission
References
Blankenship RE, Cheng P, Causgrove TP, Brune DC, Wang SHH, Choh JU and Wang J (1993) Redox regulation of energy transfer efficiency in antennas of green photosynthetic bacteria. Photochem Photobiol 57: 103–107
Blankenship RE, Olson JM and Miller M (1995) Antenna complexes from green photosynthetic bacteria. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 399–435. Kluwer Academic Publishers, Dordrecht
Chachisvilis M, Pullerits T, Jones MR, Hunter CN and Sundström V (1994) Coherent nuclear motions and exciton-state dynamics in photosynthetic light-harvesting pigments. In: Barbara PF, Knox WH, Mourou GA and Zewail A (eds) Ultrafast Phenomena IX, Proceedings of the 9th International Conference, Dana Point, CA, May 2–6, 1994, pp 435–436. Springer-Verlag, Berlin
Johnson SG and Small GJ (1991) Excited state structure and energy transfer dynamics of the bacteriochlorophyll a protein from Prosthecochloris aestuarii. J Phys Chem 95: 471–479
Lu XY and Pearlstein RM (1993) Simulations of Prosthecochloris bacteriochlorophyll a protein optical spectra improved by parametric computer search. Photochem Photobiol 57: 86–91
Lyle PA and Struve WS (1990) Evidence for ultrafast exciton localization in the Qy band of bacteriochlorophyll a-protein from Prosthecochloris aestuarii. J Phys Chem 94: 7338–7339
Matthews BW and Fenna RE (1980) Structure of a green bacteriochlorophyll protein. Acc Chem Res 13: 309–317
Matthews BW, Fenna RE, Bolognesi MC, Schmid MR and Olson JM (1979) Structure of a bacteriochlorophyll a-protein from the green photosynthetic bacterium Prosthecochloris aestuarii. J Mol Biol 131: 259–285
Olson JM (1980) Bacteriochlorophyll a-proteins of two green photosynthetic bacteria. Methods Enzymol 69: 336–344
Pearlstein RM (1992) Theory of the optical spectra of the bacteriochlorophyll a protein trimer from Prosthecochloris aestuarii. Photosynth Res 31: 213–226
Reddy NRS, Jankowiak R and Small GJ (1995) High-pressure hole-burning studies of the bacteriochlorophyll a antenna complex from Chlorobium tepidum. J Phys Chem 99: 16168–16178
Savikhin S (1995) Shot-noise-limited detection of absorbance changes induced by subpicojoule laser pulses in optical pumpprobe experiments. Rev Sci Instrum 66: 4470–4474
Savikhin S and Struve WS (1994) Ultrafast energy transfer in FMO trimers from the green bacterium Chlorobium tepidum. Biochemistry 33: 11200–11208
Savikhin S, Zhou W, Blankenship RE and Struve WS (1994a) Femtosecond energy transfer and spectral equilibration in bacteriochlorophyll a-protein trimers from the green bacterium Chlorobium tepidum. Biophys J 66: 110–114
Savikhin S, van Amerongen H, Kwa SLS, van Grondelle R and Struve WS (1994b) Low-temperature energy transfer in LHC-II trimers from the Chl a/b light-harvesting antenna of Photosystem II. Biophys J 66: 1597–1603
Savikhin S, Zhu Y, Lin S, Blankenship RE and Struve WS (1994c) Femtosecond spectroscopy of chlorosome antennas from the green photosynthetic bacterium Chloroflexus aurantiacus. J Phys Chem 98: 10322–10334
Tronrud DE, Schmid MR and Matthews BW (1986) Structure and X-ray amino acid sequence of a bacteriochlorophyll a protein from Prosthecochloris aestuarii refined at 1.9 Å resolution. J Mol Biol 188: 443–454
Van Grondelle R, Dekker JP, Gillbro T and Sundström V (1994) Energy transfer and trapping in photosynthesis. Biochim Biophys Acta 1187: 1–65
Vos MH, Rappaport F, Lambry JC, Breton J and Martin JL (1993) Visualization of coherent nuclear motion in a membrane protein by femtosecond spectroscopy. Nature 363: 320–325
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Savikhin, S., Struve, W.S. Low-temperature energy transfer in FMO trimers from the green photosynthetic bacterium Chlorobium tepidum . Photosynth Res 48, 271–276 (1996). https://doi.org/10.1007/BF00041018
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DOI: https://doi.org/10.1007/BF00041018