Skip to main content
SpringerLink
Log in

Thermoluminescence studies on the function of Photosystem II in the desiccation tolerant lichen Cladonia convoluta

  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

The effect of desiccation and rehydration on the function of Photosystem II has been studied in the desiccation tolerant lichen Cladonia convoluta by thermoluminescence. We have shown that in functional fully hydrated thalli thermoluminescence signals can be observed from the recombination of the S2(3)QB − (B band), S2QA − (Q band), Tyr-D+QA − (C band) and Tyr-Z+(His+)QA − (A band) charge stabilization states. These thermoluminescence signals are completely absent in desiccated thalli, but rapidly reappear on rehydration. Flash-induced oscillation in the amplitude of the thermoluminescence band from the S2(3)QB − recombination shows the usual pattern with maxima after 2 and 6 flashes when rehydration takes place in light. However, after rehydration in complete darkness, there is no thermoluminescence emission after the 1 st flash, and the maxima of the subsequent oscillation are shifted to the 3rd and 7th flashes. It is concluded that desiccation of Cladonia convoluta converts PS II into a nonfunctional state. This state is characterized by the lack of stable charge separation and recombination, as well as by a one-electron reduction of the water-oxidizing complex. Restoration of PS II function during rehydration can proceed both in the light and in darkness. After rehydration in the dark, the first charge separation act is utilized in restoring the usual oxidation state of the water-oxidizing comples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Chl:

chlorophyll

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

DT:

desiccation tolerant

PS II:

Photosystem II

TL:

thermoluminescence

P680 :

reaction center Chl of PS II

QA and QB :

puinone electron acceptors of PS II

S0,...,S4 :

the redox states of the water-oxidizing complex

Tyr-Z and Tyr-D:

redox-active tyrosine electron donors of PS II

References

  • Andersson B and Styring S (1991) Photosystem II: Molecular organization, function, and acclimation. Curr Topics Bioenerg 16: 1–81

    Google Scholar 

  • Arnold W (1968) Light reaction in green plant photosynthesis. A method of study. Science 154: 1046–1049

    Google Scholar 

  • Arnold W and Sherwood HK (1957) Are chloroplasts semiconductors? Proc Natl Acad Sci USA 43: 105–114

    Google Scholar 

  • Aro E-M, Virgin I and Andersson B (1993) Photoinhibition of Photosystem II. Inactivation, protein damage and turnover. Biochim Biophys Acta 1143: 113–134

    PubMed  Google Scholar 

  • Bewley DJ (1979) Physiological aspects of desiccation tolerance. Ann Rev Plant Physiol 30: 195–238

    Article  Google Scholar 

  • Bilger W, Rimke S, Schreiber U and Lange OL (1989) Inhibition of energy-transfer to Photosystem II in lichens by dehydration: Different properties of reversibility with green and blue-green phycobionts. J Plant Physiol 34: 261–268

    Google Scholar 

  • Debus RJ (1992) The manganese and calcium ions of photosynthetic oxygen evolution. Biochim Biophys Acta 1102: 269–352

    PubMed  Google Scholar 

  • Demeter S and Govindjee (1984) Thermoluminescence in plants. Physiol Plant 75: 121–130

    Google Scholar 

  • Demeter S and Vass I (1984) Charge accumulation and recombination in Photosystem II studied by thermoluminescence. I. Participation of the primary acceptor Q and secondary acceptor B in the generation of thermoluminescence of chloroplasts. Biochim Biophys Acta 764: 24–32

    Google Scholar 

  • Demeter S, Droppa M, Vass I and Horváth G (1982) The thermoluminescence of chloroplasts in the presence of Photosystem II herbicides. Photobiochem Photobiophys 4: 163–168

    Google Scholar 

  • Demeter S, Rózsa Zs, Vass I and Sallai A (1985) Thermoluminescence study of charge recombination in Photosystem II at low temperatures. I. Characterization of the Zv and A thermoluminescence bands. Biochim Biophys Acta 809: 369–378

    Google Scholar 

  • Demeter S, Goussias C, Bernát G, Kovács L and Petrouleas V (1993) Participation of the g=1.9 and g=1.82 EPR forms of the semiquinone-iron complex, QA −Fe2+ of Photosystem II in the generation of the Q and C thermoluminescence bands, respectively. FEBS Lett 336: 352–356

    Article  PubMed  Google Scholar 

  • De Vault D, Govindjee and Arnold W (1983) Energetics of photosynthetic glow peaks. Proc Natl Acad Sci USA 80: 985–987

    Google Scholar 

  • Govindjee, Koike H and Inoue Y (1985) Thermoluminescence and oxygen evolution from thermophilic blue-green alga obtained after single-turnover light flashes. Photochem Photobiol 42: 579–585

    Google Scholar 

  • Inoue (1995) Photosynthetic thermoluminescence as a simple probe of Photosystem II electron transport. In: Amesz J and Hoff AJ (eds) Biophysical Techniques in Photosynthesis, pp 93–107. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Johnson GN, Boussac A and Rutherford AW (1994) The origin of 40–50°C thermoluminescence bands in Photosystem II. Biochim Biophys Acta 1184: 85–92

    Google Scholar 

  • Kramer DM, Roffey RA, Govindjee and Sayre RT (1993) The AT thermoluminescence band from Chlamydomonas reinhardtii and the effects of mutagenesis of histidine residues on the donor side of the Photosystem II D1 polypeptide. Biochim Biophys Acta 1185: 228–237

    Google Scholar 

  • Ono TA and Inoue Y (1991) Biochemical evidence for histidine oxidation in Photosystem II depleted of the Mn-cluster for O2-evolution. FEBS Lett 278: 183–186

    Article  PubMed  Google Scholar 

  • Proctor MCF (1990) The physiological basis of bryophyte production. Bot J Linnean Soc 104: 61–77

    Google Scholar 

  • Renger G (1993) Water cleavage by solar radiation-an inspiring challenge of photosynthesis research. Photosynth Res 38: 229–247

    Google Scholar 

  • Rutherford AW, Crofts AR and Inoue Y (1982) Thermoluminescence as a probe of Photosystem II photochemistry. The origin of the flash-induced glow peaks. Biochim. Biophys Acta 767: 548–556

    Google Scholar 

  • Sane PV and Rutherford AW (1986) Thermoluminescence from photosynthetic membranes. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by Plants and Bacteria, pp 329–361. Academic Press, New York

    Google Scholar 

  • Schonbeck MW and Bewley DJ (1981) Responses of the moss Tortula ruralis to desiccation treatments. I. The effect of minimum water content and rates of dehydration and rehydration. Can J Bot 59: 2698–2706

    Google Scholar 

  • Tuba Z, Csintalan Zs, Nagy Z, Szente K and Takács Z (1994) Sampling of terricolous lichen and moss species for trace element analysis with special reference to bioindication of air pollution. In: Markert B (ed) Sampling of Environmental Materials for Trace Analysis, pp 297–311. VCH Publishers, Weinheim, New York, Tokyo

    Google Scholar 

  • Tuba Z, Csintalan Zs and Proctor MCF (1995) Photosynthetic responses of a moss, Tortula ruralis (Hedw.) Gaertn et al. ssp. ruralis, and the lichens Cladonia convoluta (Lam.) P. Cout. and C. furcata (Huds.) Schrad. to water deficit and short periods of desiccation, and their eco-physiological significance: A baseline study at present-day CO2 concentration. New Phytol (in press)

  • Vass I and Govindjee (1996) Thermoluminescence from the photosynthetic apparatus. Photosynthe Res 48: 117–126 (this issue)

    Google Scholar 

  • Vass I and Inoue Y (1992) Thermoluminescence in the study of Photosystem II. In: Barber J (ed) Topics in Photosysthesis. The Photosystems: Structure Function and Molecular Biology, Vol 11, pp 259–294. Elsevier, Amsterdam

    Google Scholar 

  • Vass I and Styring S (1991) pH-dependent charge equilibria between tyrosine-D and the S states in Photosystem II. Estimation of relative midpoint redox potentials. Biochemistry 30: 830–839

    PubMed  Google Scholar 

  • Vass I, Horváth G, Herczeg T and Demeter S (1981) Photosynthetic energy conversion investigated by thermoluminescence. Activation energies and half-lives of thermoluminescence bands of chloroplasts determined by mathematical resolution of glow curves. Biochim Biophys Acta 634: 140–152

    PubMed  Google Scholar 

  • Vermaas WFJ, Renger G and Dohnt G (1984) The reduction of the oxygen-evolving system in chloroplasts by thylakoid components. Biochim Biophys Acta 764: 194–202

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary

    László Sass & Imre Vass

  2. Plant Physiological Section, Department of Botany and Plant Physiology, Agricultural University of Gödöllö, Gödöllö, Hungary

    Zsolt Csintalan & Zoltán Tuba

Authors
  1. László Sass
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zsolt Csintalan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zoltán Tuba
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Imre Vass
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sass, L., Csintalan, Z., Tuba, Z. et al. Thermoluminescence studies on the function of Photosystem II in the desiccation tolerant lichen Cladonia convoluta . Photosynth Res 48, 205–212 (1996). https://doi.org/10.1007/BF00041010

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00041010

Key words

Search

Navigation