Skip to main content
SpringerLink
Log in

Effect of the redox state of QB on electric field-induced charge recombination in Photosystem II

  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Electric field-induced charge recombination in Photosystem II (PS II) was studied in osmotically swollen spinach chloroplasts (‘blebs’) by measurement of the concomitant chlorophyll luminescence emission (electroluminescence). A pronounced dependence on the redox state of the two-electron gate QB was observed and the earlier failure to detect it is explained. The influence of the QB/QB − oscillation on electroluminescence was dependent on the redox state of the oxygen evolving complex; at times around one millisecond after flash illumination a large effect was observed in the states S2 and S3, but not in the state ‘S4’ (actually Z+S3). The presence of the oxidized secondary electron donor, tyrosine Z+, appeared to prevent expression of the QB/QB − effect on electroluminescence, possibly because this effect is primarily due to a shift of the redox equilibrium between Z/Z+ and the oxygen evolving complex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BSA:

bovine serum albumin

EDTA:

ethylene-diaminetetraacetic acid

EL:

electroluminescence

FCCP:

carbonylcyanide p-trifluoromethyloxyphenyl-hydrazone

HEPESI:

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

I:

primary electron acceptor

MOPS:

3-(N-morpholino) propane sulfonic acid

P680:

primary electron donor of Photosystem II

P700:

primary electron donor of Photosystem I

QA and QB :

secondary and tertiary electron acceptors of Photosystem II

Z:

secondary electron donor (D1 Tyr 161)

References

  • Arnold WA and Azzi R (1971) The mechanism of delayed light production by photosynthetic organisms and a new effect of electric fields on chloroplasts. Photochem Photobiol 14: 233–240

    Google Scholar 

  • Bouges-Bocquet B (1973) Limiting steps in Photosystem II and water decomposition in Chlorella and spinach chloroplasts. Biochim Biophys Acta 292: 772–785

    PubMed  Google Scholar 

  • Debus RJ (1992) The manganese and calcium ions of photosynthetic oxygen evolution. Biochim Biophys Acta 1102: 269–352

    PubMed  Google Scholar 

  • De Grooth BG, van Gorkom HJ and Meiburg RF (1980) Electrochromic absorbance changes in spinach chloroplasts induced by an external electrical field. Biochim Biophys Acta 589: 299–314

    PubMed  Google Scholar 

  • Dekker JP, van Gorkom HJ, Wensink J and Ouwehand L (1984) Absorbance difference spectra of the successive redox states of the oxygen-volving apparatus of photosynthesis. Biochim Biophys Acta 767: 1–9

    Google Scholar 

  • Ellenson JL and Sauer K (1976) The electrophotoluminescence of chloroplasts. Photochem Photobiol 23: 113–123

    PubMed  Google Scholar 

  • Haumann M and Junge W (1994) The rates of proton uptake and electron transfer at the reducing side of Photosystem II in thylakiods. FEBS Lett 347: 45–50

    Article  PubMed  Google Scholar 

  • Itoh S (1978) Membrane surface potential and the reactivity of the system II primary electron acceptor to charged electron carriers in the medium. Biochim Biophys Acta 504: 324–340

    PubMed  Google Scholar 

  • Joliot P and Kok B (1975) Oxygen evolution in photosynthesis. In: Govindjee (ed) Bioenergetics of Photosynthesis, pp 387–412. Academic Press, New York

    Google Scholar 

  • Lavergne J (1987) Optical-difference spectra of the S-state transitions in the photosynthetic oxygen-evolving complex. Biochim Biophys Acta 894: 91–107

    Google Scholar 

  • Lavergne J and Etienne AL (1980) Prompt and delayed fluorescence of chloroplasts upon mixing with dichlorophenyldimethylurea. Biochim Biophys Acta 593: 136–148

    PubMed  Google Scholar 

  • Lavergne J and Leci E (1993) Properties of inactive Photosystem II centers. Photosynth Res 35: 323–343

    Article  Google Scholar 

  • Mamedov MD, Beshta OE, Samuilov VD and Semenov AY (1994) Electrogenicity at the secondary quinone acceptor site of cyanobacterial Photosystem II. FEBS Lett 35: 96–98

    Article  Google Scholar 

  • Rappaport F, Blanchard-Desce M and Lavergne J (1994) Kinetics of electron transfer and electrochromic changes during the redox transitions of the photosynthetic oxygen-evolving complex. Biochim Biophys Acta 1184: 178–192

    Google Scholar 

  • Robinson HH and Crofts AR (1983) Kinetics of the oxidationreduction reactions of the Photosystem II quinone acceptor complex, and the pathway for deactivation. FEBS Lett 153: 221–226

    Article  Google Scholar 

  • Rutherford AW, Crofts AR and Inoue Y (1982) Thermoluminescence as a probe of Photosystem II photochemistry the origin of the flash-induced glow peaks. Biophys Biochim Acta 682: 457–465

    Google Scholar 

  • Symons M, Korenstein R and Malkin S (1985) External electric-field effects on photosynthetic vesicles. The relationship of the rapid and slow phase of electrophotoluminescence in hypotonically swollen chloroplasts to PS I and PS II activity. Biochim Biophys Acta 806: 305–310

    Google Scholar 

  • Van Gorkom HJ (1985) Electron transfer in Photosystem II. Photosynth Res 6: 97–112

    Google Scholar 

  • Van Gorkom HJ (1996) Electroluminescence. Photosynth Res 48: 107–116 (this issue)

    Google Scholar 

  • Van Gorkom HJ, Thielen APGM and Gorren ACF (1982) The secondary electron acceptor of Photosystem II. In: Trumpower BL (ed) Function of Quinones in Energy Conserving Systems, pp 13–225. Academic Press, New York.

    Google Scholar 

  • Velthuys BR (1981) Electron-dependent competition between plastoquinone and inhibitors for binding to Photosystem II. FEBS Lett 126: 277–281

    Article  Google Scholar 

  • Vos MH and van Gorkom HJ (1988) Thermodynamics of electron transport in Photosystem I studied by electric field-stimulated charge recombination. Biochim Biophys Acta 934: 293–302

    Google Scholar 

  • Vos MH, van Gorkom HJ and van Leeuwen PJ (1991) An electroluminescence study of stabilization reactions in the oxygen evolving complex of Photosystem II. Biochim Biophys Acta 1056: 27–39

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biophysics, Huygens Laboratory of the State University, Leiden, P.O. Box 9504, 2300 RA, Leiden, The Netherlands

    Petra W. Hemelrijk & Hans J. van Gorkom

Authors
  1. Petra W. Hemelrijk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hans J. van Gorkom
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hemelrijk, P.W., van Gorkom, H.J. Effect of the redox state of QB on electric field-induced charge recombination in Photosystem II. Photosynth Res 48, 197–203 (1996). https://doi.org/10.1007/BF00041009

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00041009

Key words

Search

Navigation