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The effect of cycloheximide on IAA-stimulated transport of 14C-ABA and 14C-sucrose in long pea epicotyl segments

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Abstract

The effect of cycloheximide (CH) on the indol-3yl-acetic acid (IAA)-stimulated transport of 14C-labelled abscisic acid (ABA) and 14C-labelled sucrose was studied in 110 mm long pea epicotyl segments. IAA application resulted in elongation growth of the segments. This effect was decreased by CH treatment which also reduced [14C] ABA and [14C] sucrose accumulation in the growing apical part of the segments. A reduction in [14C] IAA uptake and in protein synthesis in this part of the segments was also observed. The simultaneous inhibition of protein synthesis and reduction of [14C] ABA and [14C] sucrose transport suggests that IAA can stimulate the transport of ABA and sucrose through a protein synthesis-based elongation growth.

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Authors and Affiliations

  1. Department of Nuclear Methods, University of Agriculture, Zemědělská 1, CS-61 300, Brno, Czechoslovakia

    Vladimír Borkovec

  2. Department of Botany, University of Agriculture, Zemědělská 1, CS-61 300, Brno, Czechoslovakia

    Stanislav Procházka

Authors
  1. Vladimír Borkovec
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  2. Stanislav Procházka
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Borkovec, V., Procházka, S. The effect of cycloheximide on IAA-stimulated transport of 14C-ABA and 14C-sucrose in long pea epicotyl segments. Plant Growth Regul 8, 1–9 (1989). https://doi.org/10.1007/BF00040911

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  • DOI: https://doi.org/10.1007/BF00040911

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