Abstract
A 371 base pair segment (bordered by Hind III and Eco RI cutting sites) of wheat embryo nuclear DNA has been cloned and sequenced. It is AT-rich (68%), shares some sequence features with autonomously replicating sequence (ARS) elements, and occurs in approximately 7600 copies per haploid genome. When used as probe for blot hybridization to Hind III-digested wheat DNA, it gives an irregular series of hybridization bands. Essentially the same hybridization pattern was observed for rye DNA. It is concluded that this segment is distributed irregularly but, apparently, according to the same rule in both wheat and rye genomes.
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References
Barker RF, Harberd NP, Jarvis MG, Flavell RB: Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of wheat. J Mol Biol 201: 1–17 (1988).
Berlani RE, Walbot V, Davis RW: Sequence analysis of three fragments of maize nuclear DNA which replicate autonomously in yeast. Plant Mol Biol 11: 173–182 (1988).
Birnboim HC: A rapid alkaline extraction method for the isolation of plasmid DNA. Meth Enzymol 100: 243–255 (1983).
Dvorak J, Jue D, Lassner M: Homogenization of tandemly repeated nucleotide sequences by distance-dependent nucleotide sequence conversion. Genetics 116: 487–498 (1987).
Eckdahl TT, Bennetzen JL, Anderson JN: DNA structures associated with autonomously replicating sequences from plants. Plant Mol Biol 12: 507–516 (1989).
Flavell R: The molecular characterization and organization of plant chromosomal DNA sequences. Annu Rev Plant Physiol 31 569–596 (1980).
Grellet F, Delcasso D, Panabieres F, Delseny M: Organization and evolution of higher plant alphoid-like satellite DNA sequence. J Mol Biol 187: 495–507 (1986).
Grunstein M, Hogness DS: Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72: 3961–3965 (1975).
Hattori J, Gottlob-McHugh SG, Johnson DA: The isolation of high-molecular-weight DNA from plants. Analyt Biochem 165: 70–74 (1987).
McIntyre CL, Clarke BC, Apples R: Amplification and dispersion of repeated DNA sequences in the Triticeae. Plant Syst Evol 160: 39–59 (1988).
McIntyre CL, Clarke BC, Appels R: DNA sequence analyses of the ribosomal spacer regions in the Triticeae. Plant Syst Evol 160: 91–104 (1988).
Rigby PWJ, Dieckmann M, Rhodes C, Berg P: Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 133: 237–251 (1977).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517 (1975).
Xin Z-Y, Appels R: Occurrence of rye (Secale cereale) 350 family DNA sequences in Agropyrum and other Triticeae. Plant Syst Evol 160: 65–76 (1988).
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Dobrzańska, M., Szurmak, B. A moderately repeated DNA sequence of wheat and rye genomes. Plant Mol Biol 18, 603–605 (1992). https://doi.org/10.1007/BF00040678
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DOI: https://doi.org/10.1007/BF00040678