Abstract
We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of Ltp1 showed that it contains an open reading frame of 351 bp interrupted by a single intron of 133 bp. Transient expression assays indicated that 702 bp of the 5′ upstream region of Ltp1 is sufficient to direct aleurone-specific expression during late seed development and early germination.
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References
Arondel V, Tchang F, Baillet B, Vignols F, Grellet F, Delseny M, Kader JC, Puigdomenech P: Multiple mRNA coding for phospholipid-transfer protein from Zea mays arise from alternative splicing. Gene 99: 133–136 (1991).
Benfey PN, Ren L, Chua NH: Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant development. EMBO J 9: 1677–1684 (1990).
Bernhard WR, Sommerville CR: Coidentity of putative amylase inhibitors from barley and finger millet with phospholipid transfer proteins inferred from amino acid sequence homology. Arch Biochem Biophys 269: 695–697 (1989).
Bernhard WR, Thoma S, Botella J, Sommerville CR: Isolation of a cDNA clone for spinach lipid transfer protein and evidence that the protein is synthesized by the secretory pathway. Plant Physiol 95: 164–170 (1991).
Breu V, Guerbette F, Kader JC, Kannangara CG, Svensson B, Von Wettstein-Knowles P: A 10 kD barley basic protein transfers phosphatidylcholine from liposomes to mitochondria. Carlsberg Res Commun 54: 81–84 (1984).
Jones RL: Quantitative and qualitative changes in the endoplasmatic reticulum of barley aleurone layers. Planta 150: 70–81 (1980).
Klein TM, Gradziel T, Fromm ME, Sanford JC: Factors influencing gene delivery into Zea mays cells by high-velocity microprojectiles. Bio/technology [6: 559–563 (1988).
Knudsen S, Müller M: Transformation of the developing barley endosperm by particle bombardment. Planta (in press).
Leah R, Tommerup H, Svendsen I, Mundy J: Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J Biol Chem 266: 1564–1573 (1991).
Madrid S, von Wettstein D: Reconciling contradictory notions on lipid transfer proteins in higher plants. Plant Physiol Biochem 29: in press (1992).
Mundy J, Rogers JC: Selective expression of a probable amylase/protease inhibitor in barley aleurone cells: Comparison to the barley amylase/subtilisin inhibitor. Planta 169: 51–63 (1986).
Skriver K, Olsen FL, Rogers JC, Mundy J: Cis-acting elements responsive to gibberellin and its antagonist abscisic acid. Proc Natl Acad Sci USA 88: 7266–7270 (1991).
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Skriver, K., Leah, R., Müller-Uri, F. et al. Structure and expression of the barley lipid transfer protein gene Ltp1 . Plant Mol Biol 18, 585–589 (1992). https://doi.org/10.1007/BF00040674
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DOI: https://doi.org/10.1007/BF00040674