Abstract
In this study we report identification of six members of a protein kinase gene family from soybean (Glycine max L.). Two fully degenerate oligonucleotide primers corresponding to two conserved motifs (DLK-PENV and GTHEYLAPE) in the catalytic domains of eukaryotic protein serine/threonine kinases were used in a polymerase chain reaction (PCR) to amplify soybean cDNA. Sequence analysis showed that 28 of the PCR sequences represented six different putative protein serine/threonine kinases. These results not only demonstrate that catalytic domains of protein kinases are highly conserved between plants and other eukaryotes but also suggest that there are multiple genes encoding protein kinases in plants.
This is a preview of subscription content, log in via an institution to check access.
References
Berg JM: Zinc fingers and other metal-binding domains. J Biol Chem 265: 6513–6516 (1990).
Biermann B, Johnson EM, Feldman LJ: Characterization and distribution of a maize cDNA encoding a peptide similar to the catalytic region of second messenger dependent protein kinases. Plant Physiol 94: 1609–1615 (1990).
Chelsky D, Ralph R, Jonak G: Sequence requirements for synthetic peptide-mediated translocation to the nucleus. Mol Cell Biol 9: 2487–2492 (1989).
Evans RM, Hollenberg SM: Zinc fingers: Gilt by association. Cell 52: 1–3 (1988).
Feng XH, Kung SD: Diversity of the protein kinase gene family in rice. FEBS Lett 282: 98–102 (1991).
Hanks SK, Quinn AM, Hunter T: The protein kinase family: Conserved features and deduced phylogeny of the catalytic domains. Science 241: 42–52 (1989).
Haribabu B, Dottin RP: Identification of a protein kinase multigene family of Dictyostelium discoideum: molecular cloning and expression of a cDNA encoding a developmentally regulated protein kinase. Proc Natl Acad Sci USA 88: 1115–1119 (1991).
Harper JF, Sussman MR, Schaller GE, Putnam-Evans C, Charbonneau H, Harmon AC: A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science 252: 951–954 (1991).
Hunter T: A thousand and one protein kinases. Cell 50: 823–829 (1987).
Lawton MA, Yamamoto RT, Hanks SK, Lamb CJ: Molecular cloning of plant transcripts encoding protein kinase homologs. Proc Natl Acad Sci USA 86: 3140–3144 (1989).
Lin X, Feng XH, Watson JC: Differential accumulation of transcripts encoding protein kinase homologs in greening pea seedlings. Proc Natl Acad Sci USA 88: 6951–6955 (1991).
Putnam-Evans CL, Harmon AC, Palevitz BA, Fechheimer M, Cormier MJ: Calcium-dependent protein kinase is localized with F-actin in plant cells. Cell Motil Cytoskel 12: 12–22 (1989).
Silver PA: How proteins enter the nucleus. Cell 64: 489–497 (1991).
Vallee BL, Coleman JE, Auld DS: Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains. Proc Natl Acad Sci USA 88: 999–1003 (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Feng, X.H., Bottino, P.J. & Kung, S.d. Molecular identification of a soybean protein kinase gene family by using PCR. Plant Mol Biol 18, 581–584 (1992). https://doi.org/10.1007/BF00040673
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00040673