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Propagation of Gasteria croucheri Bak. from shoot producing callus

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Abstract

Leaf explants of Gasteria croucheri Bak. cultured on a modified Murashige and Skoog medium supplemented with 8.8 × 10-6M (2 mgl-1) benzyladenine and 2.7 × 10-5M (5 mgl-1) α-naphthalene acetic acid yielded a callus which produced adventitious shoots. This callus could be maintained on a medium with 1.9 × 10-5M (4 mgl-1) kinetin only and continued to produce shoots which could be rooted on a hormone free medium. Plantlets could be readily acclimatized.

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References

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Authors and Affiliations

  1. UN/CSIR Research Unit for Plant Growth and Development, Botany Department, University of Natal, 3201, Pietermaritzburg, South Africa

    Arlene D. Bayley & J. Van Staden

Authors
  1. Arlene D. Bayley
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  2. J. Van Staden
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Bayley, A.D., Van Staden, J. Propagation of Gasteria croucheri Bak. from shoot producing callus. Plant Cell Tiss Organ Cult 11, 227–231 (1987). https://doi.org/10.1007/BF00040428

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  • DOI: https://doi.org/10.1007/BF00040428

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