The isolation and fusion of protoplasts in several species of Porphyra is described. Protoplasts from all species in the present study were obtained by treating thalli with commercial protease and bacterial crude enzyme prepared in the laboratory. Protoplast fusion was accomplished by following polyethylene glycol (PEG) and electrofusion methods. In the electrofusion method, protoplasts aligned in an AC field (1 MHz, 40 V for 20 s) and subsequently fused with a DC pulse of 250 V for 40 µs yielded optimum (about 20%) binary or trinary fusion products compared to the PEG method (about 8–10%). Chromosome counts and absorption spectra of crude phycobilins of most regenerated plants were identical to one or the other of the parental types, but several chimeral thalli showed a mixture of chromosome numbers and pigmentation.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Chen, L. C.-M., 1987. Protoplast morphogenesis of Porphyra leucosticta in culture. Bot. mar. 30: 399–403.
Chen, L. C.-M., M. F. Hong & J. S. Craigie, 1988. Protoplast development from Porphyra linearis — An edible marine red alga. In K. J. Puite, J. J. M. Dons, H. J. Huizing, A. J. Kool, M. Koornneef & F. A. Krens (eds), Progress in Plant Protoplast Research. Proc. int. Protoplast Symp. (Wageningen, The Netherlands) 7: 123–124.
Frei, E. & R. D. Preston, 1964. Non-cellulosic structural polysaccharides in algal cell walls. II. Association of xylan and mannan in Porphyra umbilicalis. Proc. r. Soc. Lond. B biol. Sci. 160: 314–327.
Fujita, Y. & S. Migita, 1985. Isolation and culture of protoplasts from some seaweeds. Bull. Fac. Fish. Nagasaki Univ. 57: 39–45 (in Japanese with English summary).
Fujita, Y. & S. Migita, 1987. Fusion of protoplasts from thalli of two different color types in Porphyra yezoensis Ueda and development of fusion products. Jap. J. Phycol. 35: 201–208.
Hanic, L. A. & J. S. Craigie, 1969. Studies on the algal cuticle. J. Phycol. 5: 89–102.
Kameya, T., M. E. Horn & J. M. Widholm, 1981. Hybrid shoot formation from fused Daucus carota and D. capilifolius protoplasts. Z. Pflanzenphysiol. 104: 459–466.
Kao, K. N., F. Constable, M. R. Michayuluk & O. L. Gamborg, 1974. Plant protoplast fusion and growth of intergeneric hybrid cells. Planta 120: 215–227.
Mukai, L. S., J. S. Craigie & R. G. Brown, 1981. Chemical composition and structure of the cell walls of the conchocelis and thallus phases of Porphyra tenera (Rhodophyta). J. Phycol. 17: 192–198.
Peat, S., J. R. Turvey & D. A. Rees, 1961. Carbohydrates of the red alga, Porphyra umbilicalis. J. chem. Soc. 1961: 1590–1595.
Polne-Fuller, M. & A. Gibor, 1984. Developmental studies in Porphyra. I. Blade differentiation in Porphyra perforata as expressed by morphology, enzymatic digestion and protoplast regeneration. J. Phycol. 20: 609–616.
Provasoli, L., 1968. Media and prospects for cultivation of marine algae. In A. Watanabe & A. Hattori (eds), Culture and Collections of Algae. Proc. U.S. — Japan Con. Hakone, Jap. Soc. Pl. Physiol., Tokyo: 63–75.
Saga, N. & Y. Sakai, 1984. Isolation of protoplasts from Laminaria and Porphyra. Bull. jap. Soc. sci. Fish. 50: 1085.
Waaland, S. A. & B. A. Watson, 1980. Isolation of a cell fusion hormone from Griflithsia pacifica Kylin, a red alga. Planta 149: 493–497.
Wittmann, W., 1965. Aceto-iron-haematoxylin-chloral hydrate for chromosome staining. Stain Tech. 40: 161–164.
Zhao, H. & X. Zhan, 1981. Isolation and cultivation of the vegetative cells of Porphyra yezoensis Ueda. J. Shandong Coll. Oceanology 11: 61–66 (in Chinese with English summary).