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Somatic embryogenesis and callus production from cotyledon explants of Eastern black walnut

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Abstract

Starting at 8 weeks and continuing until 23 weeks (nut drop) after anthesis,1 m2 explants from cotyledons of immature seeds were extracted from Juglans nigra fruits. Explants were placed on Woody Plant Medium with 1 g l-1 casein hydrolysate and 30 g l-1 sucrose. The explants remained in light for 4 weeks on primary media containing a 3×3 factorial of 0.05, 0.5, or 5.0 μM thidiazuron (TDZ) and 0.1, 1.0, or 10.0 μM 2,4-d. Explants were transferred to a secondary medium containing no plant growth regulators and incubated in darkness for 11 weeks. The greatest number of somatic embryos was produced 8, 10, and 12 weeks after anthesis from explants on media with 0.5 or 5.0 μM TDZ and 0.1 or 1.0 μM 2,4-d. Explants produced the greatest callus volume and dry weight 10, 12, and 14 weeks after anthesis. Throughout the study, callus generally increased with increasing concentrations of both TDZ and 2,4-d.

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Abbreviations

BA:

6-benzyladenine

captan:

3a,4,7,7a-tetrahydro-2-[(trichloromethyl)thio]-1H-isoindole-1,3(2H)-dione

2,4-d :

2,4-dichlorophenoxyacetic acid

IBA:

indolebutyric acid

Physan:

n-alkyl- dimethyl-benzyl ammonium chlorides and n-alkyl-dimethyl-ethylbenzyl ammonium chlorides

TDZ-thidiazuron:

N-phenyl-N′-1,2,3-thiadiazol-5-ylurea

References

  • Beineke, WF (1989) Selection and evaluation for better varieties. In: Phelps, JE (Ed) The Continuing Quest for Quality: Proceedings of the 4th Black Walnut Symposium; 1989 July 30-August 2; Carbondale, IL (pp 145–152). Walnut Council, Indianapolis, IN

    Google Scholar 

  • Carpenter, BS (1975) Rooting black walnut cuttings with ethephon. Tree Planter's Notes 26(3): 3, 29

    Google Scholar 

  • Cornu, D (1988) Somatic embryogenesis in tissue cultures of walnut (Juglans nigra, J. major and hybrids J. nigra * J. regia). In: Ahuja, MR (Ed) Somatic Cell Genetics of Woody Plants. (pp 45–49). Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Cornu, D (1989) Walnut somatic embryogenesis: physiological and histological aspects. In: Dreyer, E, Aussenac, G, Bonnet-Masimbert, M, Dizengremel, P, Favre, JM, Garrec, JP, LeTacon, F & Martin, F (Eds) Ann. Sci. For. 46 Suppl. Forest Tree Physiology. (pp 133s-135s) Elsevier, Paris, France

    Google Scholar 

  • Driver, JA & Kuniyuki, AH (1984) In vitro propagation of paradox walnut rootstock. HortScience 19: 507–509

    Google Scholar 

  • Heile-Sudholt, CH, Huetteman, CA, Preece, JE, VanSambeek, JW & Gaffney, GR (1986) In vitro embryonic axis and seedling shoot tip culture of Juglans nigra L. Plant Cell Tiss. Org. Cult. 6: 189–197

    Google Scholar 

  • Jay-Allemand, C, DePons, V, Doumas, P, Capelli, P, Sossountzov L & Cornu, D (1991) Formation de racines in vitro à partir de cotylédons de noix (Juglans sp.): un modèle d'étude de la rhozogénèse chez les espèces ligneuses. C.R. Acad. Sci. Paris Série III 312: 369–375

    Google Scholar 

  • Jensen, WA (1962) Botanical Histochemistry, Freeman, San Francisco (p. 408)

    Google Scholar 

  • Labavitch JM & Polito VS (1985) Fruit growth and development. In: Ramos DE (Ed) Walnut Orchard Management (pp 90–94). Univ. of CA-Davis Leaflet 21410

  • Liberty Hyde Bailey Hortorium (1976) Hortus Third: a Concise Dictionary of Plants Cultivated in the United States and Canada. 3rd edition Macmillan, New York

    Google Scholar 

  • Lloyd, G & McCown, B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Comb. Proc. Intl. Plant Prop. Soc. 30: 421–427

    Google Scholar 

  • Lockley, GC (1979) The effect of several concentrations of ethephon on breaking bud dormancy in black walnut. Tree Planter's Notes 30(4): 12–15

    Google Scholar 

  • McGranahan, GH, Leslie, CA & Driver, JA (1988a) In vitro propagation of mature persian walnut cultivars. HortScience 23: 220

    Google Scholar 

  • McGranahan, GH, Leslie, CA, Uratsu, SL, Martin, LA & Dandekar, AM (1988b) Agrobacterium-mediated transformation of walnut somatic embryos and regeneration of transgenic plants. Bio/Technology 6: 800–804

    Google Scholar 

  • McGranahan, GH, Leslie, CA, Uratsu, SL & Dandekar, AM (1990) Improved efficiency of the walnut somatic embryo gene transfer system. Plant Cell Rep. 8: 512–516

    Google Scholar 

  • Merkle, SA, Wetzstein, HY & Sommer, HE (1987) Somatic embryogenesis in tissue cultures of pecan. HortScience 22: 128–130

    Google Scholar 

  • Preece, JE, VanSambeek, JW, Huetteman, CA & Gaffney, GR (1989) Biotechnology: In vitro studies with walnut (Juglans) species. In: Phelps, JE (Ed) The Continuing Quest for Quality: Proceedings of the 4th Black Walnut Symposium; 1989 July 30-August 2; Carbondale, IL (pp 159–180). Walnut Council, Indianapolis, IN

    Google Scholar 

  • Reinert, J (1973) Aspects of organization-organogenesis and embryogenesis. In: Street, HE (Ed) Plant Tissue and Cell Culture (pp 338–355). Blackwell Scientific Publications, Oxford

    Google Scholar 

  • Rink, G (1989) Recent advances in walnut tree improvement. In: Phelps, JE (Ed) The Continuing Quest for Quality: Proceedings of the 4th Black Walnut Symposium; 1989 Jyly 30-August 2; Carbondale, IL (pp 140–152). Walnut Council, Indianapolis, IN

    Google Scholar 

  • Rodríguez, R (1982) Callus initiation and root formation from in vitro culture of walnut cotyledons. HortScience 17: 195–196

    Google Scholar 

  • Sesco, JA (1989) Quality black walnut-through productive research, technology transfer, and cooperation. In: Phelps, JE (Ed) The Continuing Quest for Quality: Proceedings of the 4th Black Walnut Symposium; 1989 July 30-August 2; Carbondale, IL (pp 1–8). Walnut Council, Indianapolis, IN

    Google Scholar 

  • Shreve LW (1974) Propagation of walnut, chestnut, and pecan by rooted cuttings. Proc. 8th Central States Forest Tree Improvement Conference, Oct. 11–13, 1972. Columbia MO

  • SAS (Statistical Analysis Systems) Institute (1982) SAS Users Guide. SAS Institute, Inc., Carey, NC

    Google Scholar 

  • Steel RGD & Torrie JH (1980) Principles and Procedures of Statistics. A Biometrical Approach. 2nd Ed. McGraw-Hill

  • Tulecke, W & McGranahan, G (1985) Somatic embryogenesis and plant regeneration from cotyledons of walnut, Juglans regia L. Plant Sci. 40: 57–63

    Google Scholar 

  • Van Sambeek JW & Rink G (1982) Physiology and silviculture of black walnut for combined timber and nut production. In: 72nd Annu Rep. Northern Nut Growers Assoc. pp 100–107

  • Young, PM, Hutchins, AS & Canfield, ML (1984) Use of antibiotics to control bacteria in shoot cultures of woody plants. Plant Sci. Lett. 34: 203–209

    Google Scholar 

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Authors and Affiliations

  1. Department of Forestry, Southern Illinois University, 62901, Carbondale, IL, USA

    Mark C. Neuman

  2. Department of Plant and Soil Science, Southern Illinois University, 62901, Carbondale, IL, USA

    John E. Preece

  3. USDA Forest Service, North Central Forest Experiment Station, Southern Illinois University, 62901, Carbondale, IL, USA

    J. W. Van Sambeek

  4. Department of Forestry, Southern Illinois University, 62901, Carbondale, IL, USA

    Gerald R. Gaffney

Authors
  1. Mark C. Neuman
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  2. John E. Preece
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  3. J. W. Van Sambeek
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  4. Gerald R. Gaffney
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Neuman, M.C., Preece, J.E., Van Sambeek, J.W. et al. Somatic embryogenesis and callus production from cotyledon explants of Eastern black walnut. Plant Cell Tiss Organ Cult 32, 9–18 (1993). https://doi.org/10.1007/BF00040110

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  • DOI: https://doi.org/10.1007/BF00040110

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