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Potentials of protoplast fusion in plant breeding programmes

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References

  1. Wallin A., Glimelius K. and Eriksson T., The induction of aggregation and fusion of Daucus carota protoplasts by polyethylene glycol, Z. Pflanzen-physiol., 74, 64, 1974.

    Google Scholar 

  2. Kao K.N. and Michayluk M.R., A method for high frequency intergeneric fusion of plant protoplasts, Planta, 115, 355, 1974.

    Google Scholar 

  3. Constabel, F. and Cutler, A.J., Protoplast fusion. Plant protoplasts (Ed. L.C. Fowke, F. Constabel), 53, 1985.

  4. Senda M., Takeda J., Abe S. and nakamara T. Induction of cell fusion of plant protoplasts by electrical stimulation. Plant Cell Physiol., 20, 1441, 1979.

    Google Scholar 

  5. Zimmermann U. and Scheurich P., High frequency fusion of plant protoplasts by electric fields, Planta, 151, 26, 1981.

    Google Scholar 

  6. Tempelaar M.J. and Jones M.G.K., Fusion characteristics of plant protoplasts in electric fields, Planta, 165, 205, 1985.

    Google Scholar 

  7. Tempelaar M.J., Duyst A., De Vlas S.Y., Krol G., Symmonds C. and Jones M.G.K., Modulation and direction of the electrofusion response in plant protoplasts, Plant Science, 48, 99, 1987

    Google Scholar 

  8. Sjödin, C. and Glimelius, K., Selection for resistance to Phoma lingam using pycnospore infections of plants in vivo and sirodesmin PL. as selective agent in vitro. (This volume) 1988.

  9. Fish, N., Karp, A. and Jones, M.G.K., Production of somatic hybrids by electrofusion in Solanum. Theor. Appl. Gen. (submitted).

  10. Schweiger H.-G., Dirk J., Koop H.-U., Kranz E., Neuhaus G., Spangenberg G. and Wolff D., TAG, 73, 769, 1987.

    Google Scholar 

  11. Koop H.-U. and Schweiger H.-G., Regeneration of plants after electrofusion of selected pairs of protoplasts, Eur. J. Cell Biol., 39, 46, 1985.

    Google Scholar 

  12. Lazar, G.B., Recent developments in plant protoplast fusion and selection technology. Protoplasts 1983. 6th Int. Protoplast Symposium Basel, 1983 (Eds. J. Potrykus, C.T. Harms, A. Hinnen, R. Hütter, P.J. King, R.D. Shillito), 61, 1983.

  13. Harms, C.T. Hybridization by somatic cell fusion. Plant protoplasts (Ed. L.C. Fowke, F. Constabel), 169, 1985.

  14. Kao K.N., Chromosomal behavior in somatic hybrids of soybean — Nicotiana glauca. Mol. Gen. Genet., 150, 225, 1977.

    Google Scholar 

  15. Gleba Y.Y. and Hoffmann F., Hybrid cell lines Arabidopsis thaliana + Brassica campestris: No evidence for specific chromosome elimination, Mol. Gen. Genet., 165, 257, 1978.

    Google Scholar 

  16. Sundberg E. and Glimelius K., A method for production of interspecific hyibrids within Brassiceae via somatic hybridization, using resynthesis of Brassica napus as a model. Plant Science, 43, 155, 1986.

    Google Scholar 

  17. Galbraith D.W., Mauch T.J., Identification of fusion of plant protoplasts. II. Conditions for the reproducible fluorescence labelling of protoplasts derived from mesophyll tissue. Z. Pflanzenphysiol., 98, 129, 1980.

    Google Scholar 

  18. Puite K.J., Roest S. and Pijnacker L.P., Somatic hybrid potato plants after electrofusion of diploid Solanum tuberosum and Solanum phureja, Plant Cell Reports, 5, 262, 1986.

    Google Scholar 

  19. Galbraith, D.W., Selection of somatic hybrid cells by fluorescence-activated cell sorting. Chapt. 50, Cell Culture and Somatic Cell Genetics of Plants, Vol. 1, Academic Press, 1984.

  20. Afonso C.L., Harkins K.R., Thomas-Compton M.A., Krejci A.E., Galbraith D.W., Selection of somatic hybrid plants in Nicotiana through fluorescence-activated sorting of protoplasts, Biotechnology, 3, 811, 1985.

    Google Scholar 

  21. Glimelius K. Enrichment of somatic hybrids with flow cytometry, Newletter, 52, 2, 1987.

    Google Scholar 

  22. Sundberg, W., Landgren, M. and Glimelius, K., Fertility and chromosome stability in Brassica napus resynthesised by protoplast fusion, Theor. Appl. Genet., (in press) 1987.

  23. Fahleson, J., Råhlen, L. and Glimelius, K., Protoplast fusion between Eruca sativa and Brassica napus-analysis of regenerated plants, (This volume) 1988.

  24. Bourgin, J.P. Protoplasts and the isolation of plant mutants, Protoplast symposium Basel, 1983 (Ed. J. Potrykus, C.T. Harms, A. Hinnen, R. Hütter, P.J. King, R.D. Shillito), 43, 1983.

  25. Negrutiu I., De Brouwer D., Watts J.W., Sidorov V.I., Dirks R. and Jacobs M., Fusion of plant protoplasts: a study using auxotrophic mutants of Nicotiana plumbaginifolia, Viviani, Theor. Appl. Genet., 72, 279, 1986.

    Google Scholar 

  26. Lo Schhiavo F., Giovinazzo G. and Terzi M., 8-azaquanine resistant carrot cell mutants and their use as universal hybridizers. Mol. Gen. Genet., 192, 326, 1983.

    Google Scholar 

  27. Hamill J.D., Pental D., Cocking E.C. and Müller A.J., Production of a nitrate reductase deficient streptomycin resistant mutant of Nicotiana tabacum for somatic hybridization studies. Heredity, 50, 197, 1983.

    Google Scholar 

  28. Ye J., Hauptmann R.M., Smith A.G. and Widholm J.M., Selection of a Nicotiana plumbaginifolia universal hybridizer and its use in intergeneric somatic hybrid formation, Mol. Gen. Genet., 208, 474, 1987.

    Google Scholar 

  29. Brunold C., Krüger-Lebus S., Saul M.W., Wegmüller S. and Potrykus I., Combination of kanamycin resistance and nitrate reductase deficiency as selectable markers in one nuclear genome provides a universal somatic hybridizer in plants, Mol. Gen. Genet., 208, 469, 1987.

    Google Scholar 

  30. Toriyama K., Kameya T. and Hinata K., Selection of a universal hyibridizer in Sinapis turgida Del. and regeneration of plantlets from somatic hybrids with Brassica species, Planta, 170, 308, 1987.

    Google Scholar 

  31. Harms, C.T. Somatic hybridization by protoplast fusion. Protoplast symposium Basel, 1983 (Ed. I. Potrykus, C.T. Harms, A. Hinnen, R. Hütter, P.J. King, R.D. Shillito), 69, 1983.

  32. Saul M.W. and Potrykus I., Species-specific repetitive DNA used to identify interspecific somatic hybrids, Plant Cell Reports, 3, 65, 1984.

    Google Scholar 

  33. Imamura J., Saul M.W. and Potrykus I., X-ray irradiation promoted asymmetric somatic hybridization and molecular analysis of the protucts, Theor. Appl. Genet., 74, 445, 1987.

    Google Scholar 

  34. Sala C., Biasini M.G., Morandi C., Nielsen E., parisi B. and Sala F., Selection and nuclear DNA analysis of cell hybrids between Daucus carota and Oryza sativa, J. Plant Physiol., 118, 409, 1985.

    Google Scholar 

  35. Mouras A., Saul M.W. and Potrykus I., Localization by in situ hybridization of a low copy chimaeric resistance gene introduced into plants by direct gene transfer, Mol. Gen. Genet., 207, 204, 1987.

    Google Scholar 

  36. U.N. Genome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jap. J. Bot., 7, 389, 1935.

  37. Schenck H.R. and Röbbelen G., Somatic hybrids by fusion of protoplasts from Brassica oleracea and B. campestris. Z. Pflanzenzüchtg., 89, 278, 1982.

    Google Scholar 

  38. Terada R., Yamashita Y., Nishibayashi S. and Shimamoto K., Somatic hybrids betwen Brassica oleracea and B. campestris: selection by the use of iodoacteamide inactivation and regeneration ability, Theor. Appl. Genet., 73, 379, 1987.

    Google Scholar 

  39. Mizushima, U., Genome analysis in Brassica and allied genera. Brassica crops and wild allies. (Ed. S.Tsunoda, K. Hinata, C. Gomez-Campo), 89, 1980.

  40. Primard, C., Vedel, F., Mathieu, C., Pelletier, G. and Chèvre, A.M., Interspecific somatic hybridization between Brassica Napus and Brassica Hirta (Sinapis Alba L.), Theor. Appl. Genet., (in press) 1987.

  41. Toriyama K., Kameya T. and Hinata K., Selection of a universal hybridizer in Sinapis turgida Del. and regeneration of plantlets from somatic hybrids with Brassica species, Planta, 170, 308, 1987.

    Google Scholar 

  42. Gleba Y.Y. and Hoffmann F., “Arabidobrassica”: A novel plant obtained by protoplast fusion, Planta, 149, 112, 1980.

    Google Scholar 

  43. Toriyama K., Hinata K. and Kameya T., Production of somatic hybrid plants, “Brassicomoricandia”, through protoplast fusion between Moricandia arvensis and Brassica oleracea, Plant Science, 48, 123, 1987.

    Google Scholar 

  44. Barsby T.L., Shepard J.F., Kemble R.J. and Wong R., Somatic hybridization in the genus Solanum: S. tuberosum and S. brevidens, Plant Cell Reports, 3, 165, 1981.

    Google Scholar 

  45. Austin S., Ehlenfeldt M.K., Baer M.A. and Helgeson J.P., Somatic hybrids produced by protoplast fusion between S. tuberosum and S. brevidens: phenotypic variation under field conditions, Theor. Appl. Genet., 71, 682, 1986.

    Google Scholar 

  46. Pijnacker L.P., Ferwerda M.A., Puite K.J. and Roest S., Elimination of Solanum phureja nucleolar chromosomes in S. tuberosum + S. phureja somatic hybrids, Theor. Appl. Genet., 73, 878, 1987.

    Google Scholar 

  47. Helgeson J.P., Hunt G.J., Haberlach G.T. and Austin S., Somatic hybrids between Solanum brevidens and Solanum tuberosum: Expression of a late blight resistance gene and potato leaf roll resistance, Plant Cell Reports, 3, 212, 1986.

    Google Scholar 

  48. Gibson, R.W., Jones, M.G.K. and Fish, N., Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid S. tuberosum and S. brevidens, (submitted).

  49. Ehlenfeldt M.K. and HannemanJr. R.E., The use of endosperm balance number and 2n gametes to transfer exotic germplasm in potato, Theor. Appl. Genet., 68, 155, 1984.

    Google Scholar 

  50. Handley L.W., Nickels R.L., Cameron M.W., Moore P.P. and Sink K.C., Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicordes, Theor. Appl. Genet., 71, 691, 1986.

    Google Scholar 

  51. Rick C.M., Hybrids between lycopersicon esculentum Mill. and Solanum lycopersicordes Dun. Proc. Natl. Acad. Sci., 37, 781, 1951.

    Google Scholar 

  52. Rick C.M., Hybridization between Lycopersicon escu-lentum and Sola-num pennellii: phylogenetic and cytogenetic significance, Proc. Natl. Acad. Sci., 46, 78, 1960.

    Google Scholar 

  53. Handley L.W. and Sink K.C., Plant regeneeration of protoplasts isolated from suspension cultures of Solanum lycopersicoides. Plant Sci., 42, 201, 1985.

    Google Scholar 

  54. Tan M.-L.M.C., Somatic hybridization and cybridization in some solanaceae, Vrije Universiteit te Amsterdam, Free University Press, Amsterdam, 1987.

    Google Scholar 

  55. Kinsara A., Patnaik S.N., Cocking E.C. and Power J.B., Somatic hybrid plants of Lycopersicon esculentum Mill. and Lycopersicon peruvianum Mill., J. Plant Physiol., 125, 225, 1986.

    Google Scholar 

  56. Adams T.L. and Quiros C.F., Somatic hybridization between Lycopersicon peruvianum and L. pennellii: Regenerating ability and antibiotic resistance as selection systems, Plant Science, 40, 209, 1985.

    Google Scholar 

  57. Dudits D., Hadlaczky G.Y., Bajszár G.Y., Koncz C.S., Lázár G. and Horváth G., Plant regeneration from intergeneric cell hybrids, Plant Sci. Lett., 15, 101, 1979.

    Google Scholar 

  58. Fluhr, R., The segregation of organelles and cytoplasmic traits in higher plant somatic fusion hybrids, Protoplasts 1983. 6th Int. Protoplast symposium Basel, 1983 (Eds. J. Potrykus, C.T. Harms, A. Hinnen, R. Hütter, P.J. King, R.D. Shillito), 61, 1983.

  59. Pelletier G.R., Plant organelle genetics through somatic hybridization. Oxford Surveys of Plant Molecular & Cell biology, 3, 96, 1986.

    Google Scholar 

  60. Menczel L., Galiba G., Nagy F. and Maliga P., Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana, Genetics, 100, 487, 1982.

    Google Scholar 

  61. Dudits D., Fejér O., Hadlaczky G., Koncz X., Lázár G.B. and Horváth G., Intergeneric gene transfer mediated by plant protoplast fusion, Molec. Gen. Genet., 179, 283, 1980.

    Google Scholar 

  62. Gupta P.P., Schieder O. and Gupta M., Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion, Mol. Gen. Genet., 197, 30, 1984.

    Google Scholar 

  63. Dudits, D., Maroy, E., Preznovsky, T., Olah, Z., Gyorgyey, J. and Cella, R., Transfer of resistant traits from carrot into tobacco by asykmmetric somatic hyibridization: Regeneration of fertile plants, Proc. Natl. Acad. Sci., (in press) 1987.

  64. Bates G.W., Hasenkampf C.A., Contolini C.L. and Piastuch W.C., Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts, Theor. Appl. Genet., 74, 718, 1987.

    Google Scholar 

  65. Sidorov V.A., Zubko M.K., Kuchko A.A., Komarnitsky I.K. and Gleba Y.Y., Somatic hybridization in potato: use of γ-irradiated protoplasts of Solanum pinnatisectum in genetic reconstruction, Theor. Appl. Genet., 74, 364, 1987.

    Google Scholar 

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  1. Department of Plant Breeding, The Swedish University of Agricultural Sciences, Uppsala, Sweden

    K. Glimelius

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Glimelius, K. Potentials of protoplast fusion in plant breeding programmes. Plant Cell Tiss Organ Cult 12, 163–172 (1988). https://doi.org/10.1007/BF00040080

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