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Micropropagation of Mexican redbud, Cercis canadensis var. mexicana

Abstract

Mexican redbud (Cercis canadensis var. mexicana) shoot cultures were initiated from explants taken from both mature and juvenile stock plants. Culture conditions affecting shoot growth and proliferation and rooting of three clones were investigated. Shoot growth was best on media supplemented with 0.25% activated charcoal and solidified with 0.2% Gelrite. Four commercially available salt formulations (Anderson's rhododendron medium, WPM, MS, DKW) were tested for growth of shoot cultures, and Anderson's rhododendron basal salt mixture was superior. Axillary shoots grew from explants cultured media supplemented with a wide range of concentrations of benzyladenine and thidiazuron. Benzyladenine at 5.6–22.2 μM supported the best combination of shoot quality and number. Rooting of microshoots in vitro was best on half-strength WPM containing 6.71 μM naphthaleneacetic acid and 0.1% activated charcoal.

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Abbreviations

BA:

6-benzyladenine

IBA:

indole-3-butyric acid

2iP:

6-(γ, γ-dimethylallylamino)purine

DKW:

Driver & Kuniyuki Walnut

kinetin:

6-furfurlaminopurine

MS:

Murashige & Skoog

NAA:

α-naphthaleneacetic acid

WPM:

Woody Plant Medium

TDZ:

thidiazuron

1-phenyl-3:

(1,2,3-thidiazol-5-yl)urea

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Mackay, W.A., Tipton, J.L. & Thompson, G.A. Micropropagation of Mexican redbud, Cercis canadensis var. mexicana . Plant Cell Tiss Organ Cult 43, 295–299 (1995). https://doi.org/10.1007/BF00039959

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Key words

  • Shoot culture
  • tissue culture
  • woody plant