Abstract
Somatic embryos were induced directly on adaxial surface of cotyledonary leaves within 8–10 days of culture on Murashige and Skoog medium containing 5.37 to 10.74 μM 1 — napthaleneacetic acid and 2.22 μM benzyl adenine. Germinated embryos with shoot axes developed into complete plants after transfer onto half stength Murashige and Skoog medium containing 1.07 μM 1 — napthaleneacetic acid. Histological studies suggested direct origin of somatic embryos with broad-base attachment.
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Abbreviations
- BA:
-
benzyl adenine
- MS:
-
Murashige and Skoog
- NAA:
-
1-napthaleneacetic acid
- RH:
-
relative humidity
References
Chatterji AK & Singh HP (1993) Plant regeneration from leaf calli of safflower. Proceedings, Third Intl. Safflower Conference, June 14–18 (pp 139–143), Beijing, China
Denchev P, Velcheva M & Atanassov A (1991) A new approach for direct embryogenesis in Medicago. Plant Cell Rep. 10: 338–341
Finer JJ (1987) Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annus L.) on a high sucrose containing medium. Plant Cell Rep. 6: 372–374
George L & Rao PS (1982) In vitro multiplication of safflower (Carthamus tinctorius L.) through tissue culture. Proc. Indian natn. Sci. Acad. B 48: 791–794
Hazra S, Sathaye SS & Mascarenhas AF (1989) Direct somatic embryogenesis in peanut (Arachis hypogaea). Biotechnology. 7: 949–951
Jain RK, Chowdhury JB, Sharma DR & Friedt W (1988) Genotypic and media effects on plant regeneration from cotyledon explant cultures of some Brassica species. Plant Cell Tiss. Org. Cult. 14: 197–206
Maheswaran G & Williams EG (1984) Direct somatic embryoid formation on immature embryos of Trifolium repens. T. pratense and Medicago sativa and rapid clonal propagation of T. repens. Ann. Bot. 54: 201–211
Maheswaran G & Williams EG (1986) Primary and secondary direct somatic embryogenesis from immature zygotic embryos of Brassica campestris. J. Plant Physiol. 124: 455–463
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant. 15: 473–497
Rajendra Prasad B, Khadeer MA, Seeta P & Anwar SY (1990) Influence of genotype and cold pre-treatment on anther culture response in safflower, Carthamus tinctorius L. Indian J. Exp. Biol. 28: 924–927
Sarvesh A, Reddy TP & Kavi Kishore PB (1993) Plant regeneration from cotyledons of niger. Plant Cell Tiss. Org. Cult. 32: 131–135
Tejovathi G & Anwar SY (1984) In vitro inducton of capitula from cotyledons of Carthamus tinctorius (safflower). Plant Sci. Lett. 36: 165–168
Tejovathi G & Anwar SY (1987) Plantlet regeneration from cotyledonary cultures of safflower (Carthamus tinctorius L.) In: Reddy GM (ed) Plant Cell and Tissue Culture of Economically Important Plants (pp 347–353). Osmania University, Hyderabad, India
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Mandal, A.K.A., Chatterji, A.K. & Gupta, S.D. Direct somatic embryogenesis and plantlet regeneration from cotyledonary leaves of safflower. Plant Cell Tiss Organ Cult 43, 287–289 (1995). https://doi.org/10.1007/BF00039957
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DOI: https://doi.org/10.1007/BF00039957