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Successful cryopreservation of suspension cells by encapsulation-dehydration

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Abstract

Cryopreservation of a Catharanthus cell suspension was performed after encapsulation in alginate beads. Encapsulated cells were precultured in sucrose-enriched medium for several days, dried over silica gel, and directly cooled in liquid nitrogen. After rewarming in air at room temperature, alginate beads were placed on semi-solid culture medium. Following regrowth, beads transferred to liquid medium generated a new cell suspension. Cell survival and regrowth from cryopreserved encapsulated cells depended on preculture duration and residual water content after air-drying.

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Authors and Affiliations

  1. Cryobiologie Végétale, Université Pierre et Marie Curie, 12 Rue Cuvier, 75252, Paris Cedex 05, France

    Y. Bachiri, C. Gazeau, J. Hansz, C. Morisset & J. Dereuddre

Authors
  1. Y. Bachiri
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  2. C. Gazeau
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  3. J. Hansz
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  4. C. Morisset
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  5. J. Dereuddre
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Additional information

Jean Dereuddre unexpectedly passed away on 16 February 1995.

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Bachiri, Y., Gazeau, C., Hansz, J. et al. Successful cryopreservation of suspension cells by encapsulation-dehydration. Plant Cell Tiss Organ Cult 43, 241–248 (1995). https://doi.org/10.1007/BF00039951

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  • DOI: https://doi.org/10.1007/BF00039951

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