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Regeneration of albino shoots in cultured anthers of intermediate wheatgrass (Agropyron intermedium (Host) Beauv.)

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Abstract

Shoots were regenerated from ‘Oahe’ intermediate wheatgrass anthers cultured on Tsay's, N6, Yu-pei and 85D12 basal media supplemented with kinetin and 2,4-D or NAA. Androgenesis mainly started with symmetrical divisions of pollen nuclei immediately followed by cytokinesis. Formation of tetranucleate pollen grains resulting from asymmetrical divisions of the pollen nuclei was also noted. Tsay's medium was more effective for callus induction, while N6, Tsay's and Yu-pei differentiation media were equally effective for shoot regeneration in the calluses. All regenerants were albino.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

naphthaleneacetic acid

References

  • Ahloowalia BS (1984) Forage grasses. In: Ammirato PV, Evans DA, Sharp WR & Yamada Y (Eds) Handbook of Plant Cell Culture, Vol 3 (pp 91–125). MacMillan, New York

    Google Scholar 

  • Chu C (1978) The N6 medium and its application to anther culture of cereal crops. In: Proceedings of Symposium on Plant Tissue Culture (pp 43–50) Science Press, Peking

    Google Scholar 

  • Clapham D (1971) In vitro development of callus from the pollen of Lolium and Hordeum. Z. Pflanzenzuchtung 65: 285–292

    Google Scholar 

  • Day A & Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Current Genet. 9: 671–678

    Google Scholar 

  • Hassawi DS, Sears RG & Liang GH (1990) Microspore development in the anther culture of wheat (Triticum aestivum L.). Cytologia 55: 475–478

    Google Scholar 

  • He DG & Ouyang JW (1985) Observation of androgenesis in cultured wheat anthers at meiosis, tetrad, early uninucleate and trinucleate stage. Acta Bot. Sinica 27: 469–475

    Google Scholar 

  • Kasperbauer MJ, Buckner RC & Springer WD (1980) Haploid plants by anther-panicle culture of tall fescue. Crop. Sci. 20: 102–107

    Google Scholar 

  • Ku M, Chang W, Kuo L, Kuan Y, An H & Huang C (1978) Induction factors and morpho-cytological characteristics of pollen-derived plants in maize (Zea mays). In: Proceedings of Symposium on Plant Tissue culture (pp 35–42). Science Press, Peking

    Google Scholar 

  • Liang GH, Sangduen N, Heyne EG & Sears RG (1982) Polyhaploid production through anther culture in common wheat. J. Hered. 73: 360–364

    Google Scholar 

  • Liang GH, Xu A & Hoang T (1987) Direct generation of wheat haploids via anther culture. Crop. Sci. 27: 336–339

    Google Scholar 

  • Marburger JE & Wang RRC (1988) Anther culture of some perennial Triticeae. Plant Cell Rpt. 7: 313–317

    Google Scholar 

  • Rose JB. Dunwell JM & Sunderland N (1987a) Anther culture of Lolium temulentum, Festuca pratensis and Lolium × Festuca hybrids. I. Influence of pretreatment, culture medium and culture incubation conditions on callus production and differentiation. Ann. Bot. 60: 191–201

    Google Scholar 

  • Rose JB, Dunwell JM & Sunderland N (1987b) Anther culture of Lolium temulentum, Festuca pratensis and Lolium × Festuca hybrids. II. Anther and pollen development in vivo and in vitro. Ann. Bot. 60: 203–214

    Google Scholar 

  • Sun CS (1978) Androgenesis of cereal crops. In: Proceedings of Symposium on Plant Tissue Culture (pp 117–125). Science Press, Peking

    Google Scholar 

  • Tsay HS (1983) The breeding of all-male asparagus plants by means of tissue culture. Food Fert Tech Center Tech Bull No 72, Taiwan 15 p

  • Yao KS, Chen CH & McMullen CR (1988) Nuclear behavior of pollen grains in cultured anthers of big bluestem (Andropogon gerardii Vitman). Proc. SD. Acad. Sci. 69: 122–123

    Google Scholar 

  • Zhou HP & Konzak CF (1989) Improvement of anther culture methods for haploid production in wheat. Crop. Sci. 29: 817–821

    Google Scholar 

  • Zhuang J, Chen G & Jia X (1990) Studies on the conditions of inducing pollen plants from the hybrids of three genera Triticum. Agropyron and Secale. Acta Genet. Sinica 17: 17–22

    Google Scholar 

  • Zhuang J, Jia X, Chen G & Sun S (1985) Factors affecting the induction of pollen plants of intergeneric hybrids of Triticum aestivum × Agropyron intermedium. Theor. Appl. Genet. 70: 294–299

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology and Microbiology, South Dakota State University, 57007, Brookings, SD, USA

    Kangshen Yao, Chen H. Chen & Charles R. McMullen

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  1. Kangshen Yao
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  2. Chen H. Chen
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  3. Charles R. McMullen
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Yao, K., Chen, C.H. & McMullen, C.R. Regeneration of albino shoots in cultured anthers of intermediate wheatgrass (Agropyron intermedium (Host) Beauv.). Plant Cell Tiss Organ Cult 26, 189–193 (1991). https://doi.org/10.1007/BF00039943

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  • DOI: https://doi.org/10.1007/BF00039943

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