Abstract
Callus growth and development involve a complex relationship between the explants used to initiate callus, the constituents of the medium and the environmental conditions during culturing. Use of high molecular weight osmotica such as polyethylene glycol (PEG-4000) results in non-solidification of agar medium used for culturing and selection. Thus, a new filter paper bridge technique was compared with the existing agar medium for callus initiation, multiplication, and plant regeneration of wheat. The yield of both total and embryogenic callus was doubled and significantly higher number of regenerants was obtained on filter paper bridges compared to agar medium.
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References
Caplin SM (1947) Growth and morphology of tobacco tissue culture in vitro. Bot Gaz. 108: 379–393
Gautheret R (1942) Sur la culture des tissus de carotte et de topinambour même à l'état de lames réduites à une assise de cellules. C. R. Hebd. Seances Acad. Sci. 214: 805–807
Janes BE (1966) Adjustment mechanism of plants subjected to various osmotic pressures of nutrient solutions. Soil Sci. 101: 180–188
Kaufmann MR & Eckard AN (1971) Evaluation of water stress control with polyethylene glycol by analysis of guttation. Plant Physiol. 47: 453–458
Linsmaier EM & Skoog F (1965) Organic growth factor requirement of tobacco tissue cultures. Physiol. Plant. 18: 100–127
McClendon JH & Blinks LR (1952) The use of high molecular weight solutes in the study of isolated intracellular structures. Nature 170: 577–578
Michel BE (1970) Carbowax-6000 compared with mannitol suppressant of cucumber hypocotyl elongation. Plant Physiol. 45: 507–509
Mohmand AS & Nabors MW (1990) Somaclonal variant plants of wheat derived from mature embryo explants of three genotypes. Plant Cell Rep. 8: 558–560
Ruf RHJr, Eckard REJr & Gifford RO (1967) Compounds of osmotic adjustment of plants to rapid changes in root medium osmotic pressure. Soil Sci. 104: 159–162
Smyth HF, Carpenter CPJr & Shaffer CB (1955) The chronic oral toxicity of the polyethylene glycol. J. Amer. Pharm. Assoc. (Sci. ed.). 44: 27–30
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Mohmand, A.S., Nabors, M.W. Comparison of two methods for callus culture and plant regeneration in wheat (Triticum aestivum). Plant Cell Tiss Organ Cult 26, 185–187 (1991). https://doi.org/10.1007/BF00039942
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DOI: https://doi.org/10.1007/BF00039942