Abstract
In vitro propagation protocols were established for two leguminous trees, Bauhinia variegata and Parkinsonia aculeata. In each case axillary shoot proliferation was achieved from nodal explants from mature (6-2-8 years) trees using Murashige & Skoog's medium supplemented with 2.22–31.1 μM of 6-benzyladenine. Subsequent rooting of the regenerated shoots was achieved on medium containing 2.46–14.8 μM of indole-3-butyric acid. Successful transfer of the regenerants to soil has been accomplished.
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Bonga JM (1987) Clonal propagation of mature trees: problems and possible solutions. In: Bonga JM & Durzan DJ (Eds) Cell and Tissue Culture in Forestry, Vol 1 (pp 249–271). Martinus Nijhoff, Dordrecht
Dhawan V & Bhojwani SS (1985) IitIn vitro vegetative propagation of Leucaena leucocephala (Lam) de Wit. Plant Cell Rep. 4: 315–318
Gharyal PK & Maheshwari SC (1983) In vitro differentiation of plantlets from tissue cultures of Albizzia lebbeck L. Plant Cell Tiss. Org. Cult. 2: 49–53
Gharyal PK & Maheshwari SC (1990) Differentiation in explants from mature leguminous trees. Plant Cell Rep. 8: 550–553
Khattar S & Mohan Ram HY (1982) Organogenesis in the cultured tissues of Sesbania sesban, a leguminous shrub. Indian J. Exp. Biol. 20: 216–219
Khattar S & Mohan Ram HY (1983) Organogenesis and plantlet formation in vitro in Sesbania grandiflora. Indian J. Exp. Biol. 21: 251–253
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nataraja K & Sudhadevi AM (1984) Induction of plantlets from seedling explants of Dalbergia latifolia Roxb.: in vitro. Beitr. Biol. Pflanz. 59: 341–349
Rajadurai D, Rao AN & Loh CS (1989) In vitro studies on two leguminous species. In: Rao AN & Yusoff A Mohd (Eds) Proceedings of Seminar on Tissue Culture of Forest Species (pp 104–128). FRIM and IDRC, Kuala Lumpur
Skolmen RG & Mapes MO (1976) Acacia kao Gray plantlets from somatic callus tissue. J. Hered. 67: 114–115
Tomar UK & Gupta SC (1988) Somatic embryogenesis and organogenesis in callus cf a tree legume Albizzia richardiana. King. Plant Cell Rep. 7: 70–73
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Mathur, J., Mukunthakumar, S. Micropropagation of Bauhinia variegata and Parkinsonia aculeata from nodal explants of mature trees. Plant Cell Tiss Organ Cult 28, 119–121 (1992). https://doi.org/10.1007/BF00039924
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DOI: https://doi.org/10.1007/BF00039924