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Somatic hybridization between Nicotiana rustica and N. sylvestris

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Abstract

Protoplasts isolated from cell cultures of chlorophyll-deficient Nicotiana rustica cv. chlorotica and wild-type N. sylvestris were fused. The scheme for selection of somatic hybrids was based on the inability of the protoplast-derived colonies of the parental species to turn green; N. sylvestris protoplasts also had a very low plating efficiency in the medium employed. A total of 777 green colonies which were presumptive hybrids were isolated within four weeks of the fusion experiments. One hundred and eight green colonies formed shoots in vitro and 16 lines were rooted and grown in the greenhouse. Each of these hybrid plants displayed vegetative and floral traits intermediate to those of the parental species, except for plant height which in almost all cases was greater in the hybrids. Isozyme analyses by gel electrophoresis and isoelectric focussing of the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBPCase) demonstrated that the nuclear genomes of both parents were expressed by the hybrids. Each of the eight somatic hybrid plants analyzed expressed only the N. rustica chloroplast genome as shown by isoelectric focussing of the large subunit of RUBPCase. This study demonstrated the value of N. rustica cv. chlorotica as a parental line in somatic hybridization with N. sylvestris and it might have widespread use with wild-type lines of other species.

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Authors and Affiliations

  1. Department of Biology, Carleton University, K1S 5B6, Ottawa, Ontario, Canada

    S. Gleddie & G. Setterfield

  2. Research Station, Research Branch, Agriculture Canada, K1A OC6, Ottawa, Ontario, Canada

    W. A. Keller

  3. Prairie Regional Laboratory, National Research Council of Canada, S7N OW9, Saskatoon, Saskatchewan, Canada

    L. R. Wetter

Authors
  1. S. Gleddie
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  2. W. A. Keller
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  3. G. Setterfield
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  4. L. R. Wetter
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Contribution No. 706 Ottawa Research Station, Research Branch, Agriculture Canada, Ottawa, Ontario K1A OC6, Canada

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Gleddie, S., Keller, W.A., Setterfield, G. et al. Somatic hybridization between Nicotiana rustica and N. sylvestris . Plant Cell Tiss Organ Cult 2, 269–283 (1983). https://doi.org/10.1007/BF00039874

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  • DOI: https://doi.org/10.1007/BF00039874

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