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High frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br.


An efficient tissue culture system for high frequency of plant regeneration from hypocotyl explants of Brassica carinata was developed via manipulation of culture medium and selection of explants. Explants grown on medium containing combinations of 2 mg l-1 BA and 0.01 mg l-1 NAA or 4 mg l-1 kinetin and 0.01 mg l-1 2,4-D regenerated shoots at 100% frequency. High frequency shoot regeneration occurred only from explants originating from 6 to 7-day-old but not younger or older seedlings. Explants showed higher regeneration capacity at the distal end than the proximal end, and the upper segment was more regenerative than the lower segment of hypocotyl. Regenerants were rooted on half-strength growth regulator-free medium, acclimatized and developed into normal, fertile plants.

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2,4-dichlorophenoxyacetic acid


naphthaleneacetic acid


Murashige & Skoog


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Yang, MZ., Jia, SR. & Pua, EC. High frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br.. Plant Cell Tiss Organ Cult 24, 79–82 (1991).

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Key words

  • Brassica carinata
  • Ethiopia mustard
  • hypocotyl
  • plant regeneration
  • tissue culture