Skip to main content
Log in

Use of irradiated pollen as mentor pollen to induce self-fertilization of two self-incompatible Upper Amazon cacao clones

  • Published:
Euphytica Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Summary

Two self-incompatible Upper Amazon cacao clones, T85/799 and T79/501, were pollinated with compatible Amelonado pollen subjected to varying doses of gamma irradiation (10–100 Gy). The proportion of flat non-viable beans to fully formed, viable beans in the pods increased with an increase in dosage of gamma rays. At 60 Gy all the beans produced were flat and non-viable, beyond this dosage fruit set was zero. Pollinating the self-incompatible cacao clones with a 1 : 1 mixture of compatible mentor pollen irradiated at 60 Gy and normal self pollen produced a mixture of flat, non-viable beans and fully-formed viable beans. Similar experiments using irradiated pollen with a marker gene suggested that the fully-formed viable beans resulted from selfing. Increasing the proportion of the radiation-treated compatible pollen in the mixture increased the number of fully-formed beans. However, when compatible pollen which had been treated either at 80 Gy or with temperatures of 35° C, 40° C and 45° C for periods of five, ten and fifteen minutes in factorial combination were mixed with self pollen, no successful pollinations were achieved. Pollen viability tests indicated that, whilst pollen treated at 60 Gy were about 50% viable, those treated at either 80 Gy or with temperatures of 35–45° C were mostly not viable. This suggests that, to overcome the incompatibility in cacao, the tubes of the mentor pollen grains used should at least grow into the style. The possible causes for overcoming the self-incompatibility in cacao are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Cope, F.W., 1962. The mechanism of pollen incompatibility in Theobroma cacao L., Heredity 17: 157.

    Google Scholar 

  • Ecochard, R., M.S., Rawanna & D.De, Nettancourt, 1969. Detection and cytological analysis of tomato haploids, Genetica 40: 181–190.

    Google Scholar 

  • Glendinning, D.R., 1960. Selfing of self-incompatible cocoa, Nature 187: 170.

    Google Scholar 

  • Glendinning, D.R., Rep. 1965. CRIG. 75–86.

  • Grant, J.E., K.K., Pandey & E.G., Williams, 1980. Pollen nuclei after ionizing irradiation for egg transformation in Nicotiana, New Zealand J. Bot. 18: 339–341.

    Google Scholar 

  • Knight, R. & H.H., Rogers, 1955. Incompatibility in Theobroma cacao, Heredity 9: 69.

    Google Scholar 

  • Lanaud, C., O., Sounigo, Y.K., Amefia, D., Paulin, Ph., Lachenaud & D., Clement, 1987. Nouvelles données sur le fonctionnement du système d'incompatibilité du cacaoyer et ses conséquences pour la sélection, Café Cacao Thé 31 3: 267–277.

    Google Scholar 

  • Martinson, V.A., 1972. Rep. CRIG: 178–179.

  • Oost, E.H. & A.P.M.Den, Nijs, 1979. Storage and irradiation of Cucumis pollen and their influence on pollen vitality, Inc. Newsl. 11: 63–67.

    Google Scholar 

  • Opeke, L.K. & V.J. Jacob, 1967. Studies on methods of overcoming self-incompatibility in Theobroma cacao L., 2nd Conférence Internationale sur les Recherches Cacaoyères Salvador-Itabuna (Bahia), 19–25 November: 356–359.

  • Stettler, R.F. & R.P., Guries, 1976. The mentor pollen phenomenon in black cottonwood, Can. J. Bot. 54: 820–830.

    Google Scholar 

  • Posnette, A.F., 1945. Incompatibility in Amazon cocoa, Trop. Agric. (Trin.) 22: 184.

    Google Scholar 

  • Visser, T. & E.G., Oost, 1981. Pollen and pollination experiments III. The viability of apple and pear pollen as affected by irradiation and storage, Euphytica 30: 65–70.

    Google Scholar 

  • Van den, Boom, J.M.A. & A.P.M.Den, Nijs, 1983. Effects of gamma radiation on vitality and competitive ability of Cucumis pollen, Euphytica 32: 677–684.

    Google Scholar 

  • Voelcker, O.J., 1937. A summary of (cacao) literature since 1931, 7th Rep. Cacao Res. Trinidad: 20–30.

  • Voelcker, O.J., 1938. Self-incompatibility in cacao, 7th Rep. Cacao Res. Trinidad 11: 2–5.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Adu-Ampomah, Y., Novak, F.J., Klu, G.Y.P. et al. Use of irradiated pollen as mentor pollen to induce self-fertilization of two self-incompatible Upper Amazon cacao clones. Euphytica 51, 219–225 (1990). https://doi.org/10.1007/BF00039722

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00039722

Key words

Navigation