Summary
In one cultivar of each of Brassica napus and B. oleracea, and three out of the four cultivars tested in B. campestris, pollen remained functional after a year or more in dry storage at −20° C. The percentage of pollinations resulting in mature siliques was in some cases greater using stored pollen than after fresh pollen. The number of mature seeds per silique after pollinating with stored pollen varied with cultivar but was usually similar to, or less than, the number produced by fresh pollen. The embryos formed after pollination with stored pollen developed at the same rate as those formed by fresh pollen. There was an increase in the percentage of precocious seed germination after pollination with stored pollen.
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References
Brown, Angela, P., 1985. Pollen, embryo and endosperm development following cross-pollination within and between the crop species Brassica campestris, B. oleracea, B. napus and Raphanus sativus. Ph.D Thesis, University of Edinburgh.
Chiang, M.S., 1974. Cabbage pollen germination and longevity. Euphytica 23: 579–584.
Knox, R.B. & E.G., Williams, 1986. Pollen, pistil and reproductive functions in crop plants. Plant Breeding Reviews 4: 9–79.
Ockendon, D.J., 1974. The value of stored pollen in incompatibility studies in Brassica. Incompatibility Newsletter, No. 4: 17–19.
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Brown, A.P., Dyer, A.F. Effects of low temperature storage on the pollen of Brassica campestris, B. oleracea and B. napus . Euphytica 51, 215–218 (1990). https://doi.org/10.1007/BF00039721
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DOI: https://doi.org/10.1007/BF00039721