Abstract
A micropropagation system for Annona squamosa L. (Sugar Apple) using hypocotyls of seedlings and nodal cuttings from 3-year-old plants was developed. Shoot proliferation was achieved with Woody Plant Medium supplemented with BA. Silver thiosulphate was added at 0.5 mg l−1 to control leaf abscission. Rooting was obtained when subcultured shoots were preconditioned for 2 weeks in medium with 10 g l−1 activated charcoal before treatment with 43 µm NAA or 39 µm IBA. Rooting was improved when galactose was used instead of sucrose in the rooting medium. The rooted plantlets were acclimatised successfully.
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Abbreviations
- NAA:
-
naphthaleneacetic acid
- IBA:
-
indolebutyric acid
- MS:
-
Murashige & Skoog Medium
- WPM:
-
Woody Plant Medium
- NN:
-
Nitsch Medium
- Juv:
-
juvenile explant
- Adu:
-
adult explant
References
Lemos E E P & Blake J (1994) Leaf abscission in micropropagated sugar apple (Annona squamosa L.). In: Lumsden P J, Nicholas J R & Davies W J (eds) Physiology, Growth and Development of Plants in Culture (pp 227–232). Kluwer Academic Publishers
Lemos E E P & Blake I (1996) Control of leaf abscission in nodal cultures of Annona squamosa L. Jour. Hortic. Sci. (in press)
Lloyd G & McCown B (1981) Commercially-feasible micropropagation of Mountain laurel, Kalmia latifolia, by use of shoot tip culture. Int. Plant Prop. Soc. Proc. 30: 421–427
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plan. 15: 473–479
Nitsch J P & Nitsch C (1956) Auxin-dependent growth of excised Helianthus tissues. American Journal of Botany 43: 839–851
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Lemos, E.E.P., Blake, J. Micropropagation of juvenile and adult Annona squamosa . Plant Cell Tiss Organ Cult 46, 77–79 (1996). https://doi.org/10.1007/BF00039698
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DOI: https://doi.org/10.1007/BF00039698