Summary
Generation of Simple Sequence Repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequence repeats (SSR) and to generate polymerase chain reaction (PCR) primers based on the sequences flanking the simple sequence repeat. Twenty six different pairs of primers which yield a PCR product in the initial screening were synthesized. The SSR A1E11 was found to have eleven alleles while A3F8 has eight alleles. The SSRs in avocado were found to be inherited in a Mendelian fashion.
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Akkaya, M.S., A.A. Bhagwat & P.B. Cregan, 1992. Length polymorphism of simple sequence repeat DNA in soybean. Genetics 132: 1131–1139.
Arumuganathan, K. & E.D. Earle, 1991. Nuclear DNA content of some important plant species. Plant Mol. Biol. Rep. 9: 208–218.
Beckman, J.S. & M. Soller, 1983. Restriction fragment length polymorphism in genetic improvement: methodologies mapping and costs. Theor. Appl. Genet. 67: 35–43.
Bergh, B.O., 1975. Avocados. In: J. Janick & J.N. Moore (Eds). Advances in Fruit Breeding. Purdue Univ. Press, West Lafayette, Indiana.
Botstein, D., R.L. White, M. Skolnic & R.W. Davis, 1980. Construction of a genetic map in man using restriction fragment length polymorphism. Am. J. Human Genet. 32: 314–330.
Condit, R. & S.P. Hubbell, 1991. Abundance and DNA sequence of two-base repeat regions in tropical tree genomes. Genome 34: 66–71.
Cregan, P.B., 1992. Simple sequence repeat DNA length polymorphisms. Probe 2: 18–22.
Cregan, P.B., M.S. Akkaya & A.A. Bhagwat, 1992. Length polymorphisms of simple sequence repeat (SSR) as molecular markers in soybean. Am. Soc. Agron. Abstr. Madison, Wisconsin. p. 188.
Degani, C. & S. Gazit, 1984. Selfed and crossed proportions of avocado progenies produced by caged pairs of complementary cultivars. HortScience 19: 258–260.
Degani, C., A. Goldring, I. Adato, R. El-Batsri & S. Gazit, 1990. Pollen parent effect on outcrossing rate yield and fruit characteristics of ‘Fuerte’ avocado. HortScience 25: 471–473.
Degani, C., A. Goldring & S. Gazit, 1989. Pollen parent effect on outcrossing rate in ‘Hass’ and ‘Fuerte’ avocado plots during fruit development. J. Am. Soc. Hort. 114: 106–111.
Degani, C., A. Goldring, S. Gazit & U. Lavi, 1986. Genetics selection during abscission of avocado fruitlets. HortScience 21: 1187–1188.
Furnier, G.H., M.P. Cummings & M.T. Clegg, 1990. Evolution of the avocados by DNA restriction fragment variation. J. Hered. 81: 183–188.
Gebhardt, C. & F. Salamini, 1982. Restriction fragment length polymorphism analysis of plant genomes and its application to plant breeding. Int. Rev. Lytol. 135: 201–237.
Goldring, A., S. Gazit & C. Degani. 1987. Isozyme analysis of mature avocado embryos to determine outcrossing rate in a ‘Hass’ plot. J. Am. Soc. Hort. Sci. 112: 389–392.
Hamada, H., M.G. Petrino & T. Kakunago, 1982. A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes. Proc. Natl. Acad. Sci. USA 79: 6465–6469.
Hillel, J., T. Schaap, A. Haberfeld, A.J. Jeffreys, Y. Plotsky, A. Cahaner & U. Lavi, 1990. DNA fingerprints applied to gene introgression in breeding programs. Genetics 124: 783–789.
Keim, P., R.C. Schoemaker & R.G. Palmer, 1989. Restriction fragment length polymorphism diversity in soybean. Theor. Appl. Genet. 77: 787–792.
Lagererantz, U., H. Ellengren & L. Anderson, 1993. The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates. Nucleic Acids Res. 21: 1111–1115.
Lavi, U., J. Hillel, A. Vainstein, E. Lahav & D. Sharon, 1991. Application of DNA fingerprints for identification and genetic analysis of avocado (Persea americana). J. Am. Soc. Hort. Sci. 116: 1078–1081.
Lavi, U., E. Lahav, A. Genizi, G. Degani, S. Gazit & J. Hillel, 1990. Quantitative genetic analysis of avocado traits and cultivars. Plant Breed. 106: 149–160.
Litt, M. & J.A. Luty, 1989. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Hum. Genet. 44: 397–401.
Morgante, M. & A.M. Olevieri, 1993. PCR-amplified microsatellites as markers in plant genetics. Plant J. 3: 175–182.
NIH/CEPH Collaborative Mapping Group, 1992. A comprehensive genetic linkage map of the human genome. Science 258: 67–86.
Rongwen, J., M.S. Akkaya, A.A. Bhaywat, U. Lavi & P.B. Cregan, 1994. The use of simple sequence repeat DNA markers for soybean genotype identification. Theor. Appl. Genet. (in press).
Rostas, K., E. Kondorosi, B. Horvath, A. Simoncsits & A. Kondorosi, 1986. Conservation of extended promoter regions of nodulation genes in Rhizobium. Proc. Natl. Acad. Sci. USA 83: 1757–1761.
Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular cloning: a laboratory manual. Second Edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Senior, M.L. & M. Heum, 1993. Mapping maize microsatellites and polymerase chain reaction confirmation of targeted repeats using a CT primer. Genome 36: 884–889.
Stallings, R.L., D.C. Torney, C.E. Hildebrand, J.L. Longmire, L.L. Deaven, J.H. Jett, N.A. Doggett & R.K. Moyzis, 1990. Physical mapping of human chromosomes by repetitive sequence finger-printing. Proc. Natl. Acad. Sci. USA 87: 6218–6222.
Tautz, D., 1989. Hypervariability of simpe sequences as a general source of polymorphic DNA markers. Nucleic Acids Res. 17: 6463–6471.
Weber, J.K. & P.E. May, 1989. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Hum. Genet. 44: 388–396.
Weissenbach, J., G. Gyapay, C. Dib, A. Vignal, J. Morissette, P. Millasseau, G. Vaysseix & M. Lathrop, 1992. A second-generation linkage map of the human genome. Nature 359: 794–801.
Zhao, X. & G. Rochert, 1993. Phylogenetic distribution and genetic mapping of a (GGC)n microsatellite from rice (Oryza sativa L.). Plant Mol. Biol. 21: 614–617.
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Contribution from the Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel. No. 1335-E, 1994 series.
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Lavi, U., Akkaya, M., Bhagwat, A. et al. Methodology of generation and characteristics of simple sequence repeat DNA markers in avocado (Persea americana M.). Euphytica 80, 171–177 (1994). https://doi.org/10.1007/BF00039648
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DOI: https://doi.org/10.1007/BF00039648