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A rapid laboratory method for cytological control in polyploidy breeding

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Summary

A simple method to obtain material for cytological checking in grasses and clovers is described. Seeds are germinated on filter paper under optimal conditions. When the rootlets are about 5 mm long they are fixed in acetic acid alcohol 1–3. The chromosome number is determined by phase-contrast microscope and the tetraploids are potted. Advantages of this method are illustrated.

Samenvatting

Het is in vele gevallen wenselijk cytologisch contrôle onderzoek te verrichten aan de nakomelingschap van met colchicine behandelde planten.

De daartoe govelgde methode, het uitzaaien en oppotten van het te onderzoeken materiaal en cytologisch onderzoek aan worteltoppen van de potkluit, heeft verschillende nadelen.

Volgens een nieuw ontwikkelde methode wordt zaad op rondfilters in petrischalen bij optimale omstandigheden te kiemen gelegd. De worteltoppen worden gefixeerd in azijnzure alcohol 1–3. De plantjes die in aanmerking komen voortgekweekt te worden kunnen na het onderzoek worden opgepot.

De voordelen van deze methode worden toegelicht.

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7. References

  1. Bragdø, Marie, Production of polyploids by colchicine, Euphytica 4 (1955): 76–82.

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  2. Kloen, D. and Speckmann, G. J., The creation of tetraploid beets. II. Selection in the first generation (the C1) from the treated material. Euphytica 3 (1954): 35–42.

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  3. Bremer-Reinders, D. E. and Bremer, G., Methods used for producing polyploid agricultural plants. Euphytica 1 (1952): 87–94.

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Speckmann, G.J. A rapid laboratory method for cytological control in polyploidy breeding. Euphytica 4, 163–166 (1955). https://doi.org/10.1007/BF00039641

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  • DOI: https://doi.org/10.1007/BF00039641

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