Abstract
The I locus controls inhibition of anthocyanin accumulation in the epidermal cells of the soybean seed coat and affects abundance of PRP1, a proline-rich cell wall protein in the seed coat. Saline-soluble PRP1 is abundant in the developing seed coats of cultivar Richland (homozygous I, yellow), while it is significantly decreased in the pigmented isogenic mutant T157 (homozygous i, imperfect black). In this report, we examined soluble PRP1 in several cultivars containing alleles of the I locus which affect spatial distribution of pigmentation in the seed coat. We also characterized PRP1 in isolines with allelic variants of several other loci involved in seed coat pigmentation, including T and Im. The T gene is pleiotropic and affects both pubescence color and seed coat pigmentation and structure. Soluble PRP1 was abundant in the developing seed coats of lines with yellow seed (I or i i alleles) regardless of pubescence color, just as in Richland. Likewise, soluble PRP1 was decreased in pigmented seed coats (i k or i alleles) with grey (t) pubescence, as in T157. However, the total seed coat proteins were not extractable from pigmented seed coats with tawny pubescence (i, T genotypes) because they have proanthocyanidins that exhibit tannin properties. The dominant Im allele inhibits seed coat mottling (irregular patches of pigmentation) that occurs if plants are infected with soybean mosaic virus. PRP1 was 35 kDa in mottled (im) isolines and 34 kDa in non-mottled (Im) isolines. PRP2, which is expressed later in seed coat development and in the hypocotyl hooks of soybean seedlings, was also smaller in Im isolines. In summary, some of the anthocyanin mutations affect the quantity of soluble PRP1 polypeptides, while others correlate with structural changes in developmentally regulated proline-rich proteins.
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References
Averyhart-Fullard V, Datta K, Marcus A: A hydroxyproline-rich protein in the soybean cell wall. Proc Natl Acad Sci USA 85: 1082–1085 (1988).
Bernard RL, Weiss MG: Qualitative genetics. In: Caldwell BE (ed) Soybeans: Improvement, Production, and Uses, 1st ed, pp. 117–149. American Society of Agronomy, Madison, WI (1973).
Buttery BR, Buzzell RI: Varietal differences in leaf flavonoids of soybeans. Crop Sci 13: 103–106 (1973).
Buzzell RI, Buttery BR, MacTavish DC: Biochemical genetics of black pigmentation of soybean seed. J Hered 78: 53–54 (1987).
Cooper RL: A major gene for resistance to seed coat mottling in soybean. Crop Sci 6: 290–292 (1966).
Datta K, Schmidt A, Marcus A: Characterization of two soybean repetitive proline-rich proteins and a cognate cDNA from germinated axes. Plant Cell 1: 945–952 (1989).
Hong JC, Nagao RT, Key JL: Characterization and sequence analysis of a developmentally regulated putative cell wall protein gene isolated from soybean. J Biol Chem 262: 8367–8376 (1987).
Hong JC, Nagao RT, Key JL: Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell 1: 937–943 (1989).
Hong JC, Nagao RT, Key JL: Characterization of a proline-rich cell wall protein gene family of soybean. J Biol Chem 265: 2470–2475 (1990).
Kleis-San Francisco SM, Tierney ML: Isolation and characterization of a proline-rich cell wall protein from soybean seedlings. Plant Physiol 94: 1897–1902 (1990).
Laemmli UK: Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680–685 (1970).
Lindstrom JT, Vodkin LO: A soybean cell wall protein is affected by seed color genotype. Plant Cell 3: 561–571 (1991).
Marcus A, Greenberg J, Averyhart-Fullard V: Repetitive proline-rich proteins in the extracellular matrix of the plant cell. Physiol Plantarum 81: 273–279 (1991).
Palmer RG, Kilen TC: Qualitative genetics and cytogenetics. In: Wilcox JR (ed) Soybeans: Improvement, Production, and Uses, 2nd ed. Agronomy 16: 135–209 (1987).
Tierney ML, Varner JE: The extensins. Plant Physiol 84: 1–2 (1987).
Wilcox JR: Performance and use of seedcoat mutants in soybean. Crop Sci 28: 30–32 (1988).
Wilcox JR, Laviolette FA: Seedcoat mottling response of soybean genotypes to infection with soybean mosaic virus. Phytopathology 58: 1446–1447 (1968).
Wyatt RE, Nagao RT, Key JL: Patterns of soybean proline-rich protein gene expression. Plant Cell 4: 99–110 (1992).
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Nicholas, C.D., Lindstrom, J.T. & Vodkin, L.O. Variation of proline rich cell wall proteins in soybean lines with anthocyanin mutations. Plant Mol Biol 21, 145–156 (1993). https://doi.org/10.1007/BF00039625
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DOI: https://doi.org/10.1007/BF00039625