, Volume 54, Issue 2, pp 155–159 | Cite as

Anther callus induction and green plant regeneration at high frequencies from an interspecific rice hybrid Oryza sativa Linn. x O. rufipogon Griff

  • J. R. Rout
  • N. P. Sarma


Callus induction and green plant regeneration at high frequencies from an interspefic hybrid, Oryza sativa L. x O. rufipogon Griff. has been achieved by simply coordinating the growth regulators in the induction medium. The study was conducted with two different basal media (Potato-2 and N6) and seven different combinations of growth regulators 2,4-D, NAA and kinetin. Synergistic effects of the two auxins in enhanced anther response to callus induction and subsequent green plant regeneration were observed in both media. The highest frequency of callus induction was obtained on Potato-2 medium supplemented with 1 mg/12,4-D, 2 mg/l NAA and 1 mg/l kinetin. The same combination of growth regulators which yielded higher frequencies of callus induction also induced higher mean number of calli per anther. Although the calli formed on N6 medium showed high regenerability, there was a concomitant increase in the number of albinos among the regenerants. The auxins in the induction media had considerable influence on the regeneration capacity of the calli. The regeneration frequencies were higher from calli formed in the presence of both auxins in the induction media. The levels of growth regulator combinations seem to influence the green plant regeneration especially for calli induced on Potato-2 medium. Among the pollen grain derived plants the majority were either haploids or double haploids and very few were chromosomal variants.

Key words

Oryza sativa L. Oryza rufipogon Griff. interspecific rice hybrid anther culture Potato-2 medium N6 medium growth regulators 


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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • J. R. Rout
    • 1
  • N. P. Sarma
    • 2
  1. 1.Department of BotanyUniversity of CaliforniaDavisUSA
  2. 2.Directorate of Rice ResearchHyderabadIndia

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