Advertisement

Euphytica

, Volume 54, Issue 2, pp 155–159 | Cite as

Anther callus induction and green plant regeneration at high frequencies from an interspecific rice hybrid Oryza sativa Linn. x O. rufipogon Griff

  • J. R. Rout
  • N. P. Sarma
Article
  • 46 Downloads

Summary

Callus induction and green plant regeneration at high frequencies from an interspefic hybrid, Oryza sativa L. x O. rufipogon Griff. has been achieved by simply coordinating the growth regulators in the induction medium. The study was conducted with two different basal media (Potato-2 and N6) and seven different combinations of growth regulators 2,4-D, NAA and kinetin. Synergistic effects of the two auxins in enhanced anther response to callus induction and subsequent green plant regeneration were observed in both media. The highest frequency of callus induction was obtained on Potato-2 medium supplemented with 1 mg/12,4-D, 2 mg/l NAA and 1 mg/l kinetin. The same combination of growth regulators which yielded higher frequencies of callus induction also induced higher mean number of calli per anther. Although the calli formed on N6 medium showed high regenerability, there was a concomitant increase in the number of albinos among the regenerants. The auxins in the induction media had considerable influence on the regeneration capacity of the calli. The regeneration frequencies were higher from calli formed in the presence of both auxins in the induction media. The levels of growth regulator combinations seem to influence the green plant regeneration especially for calli induced on Potato-2 medium. Among the pollen grain derived plants the majority were either haploids or double haploids and very few were chromosomal variants.

Key words

Oryza sativa L. Oryza rufipogon Griff. interspecific rice hybrid anther culture Potato-2 medium N6 medium growth regulators 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chen Y., 1986. Anther and pollen culture of rice. p. 3–25. In: H. Hu & H. Yang (Eds). Haploids of higher plants in vitro. China Academic Pub., Beijing and Springer, Berlin, Heidelberg, New York, Tokyo.Google Scholar
  2. Chu C.C., C.C. Wang, C.S. Sun, C. Hsu, K.C. Yin, C.Y. Chu & F.Y. Bi, 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica 16: 659–688.Google Scholar
  3. Huang C.C., T. Ouyang, M. Chia, S. Chou & G. Ching, 1978. A set of potato media for wheat anther culture. p. 51–56. In: Proc. symp. plant tissue culture. Science press, Beijing.Google Scholar
  4. Gomez K.A. & A.A. Gomez, 1976. Statistical procedures for agricultural research with emphasis on rice. The International Rice Research Institute. Manila, Philippines.Google Scholar
  5. Huang C.R., Y.H. Wu & C.C. Chen, 1986. Effects of plant growth substances on callus formation and plant regeneration in anther culture of rice. p. 763–771. In: Rice genetics. The International Rice Research Institute, Manila, Philippines.Google Scholar
  6. Liang H.M., 1978. The advance of studies on medium for anther culture of rice in China. p. 57–64. In: Proc. symp. plant tissue culture. Science press, Peking.Google Scholar
  7. Nishi T. & S. Mitsuoka, 1969. Plants from anther and ovary culture of rice plant. Jap. J. Genet. 44: 341–346.Google Scholar
  8. Reddy V.S., S. Leelavathi & S.K. Sen, 1985. Influence of genotype and culture medium on microspore callus induction and green plant regeneration in anthers of Oryza sativa. Physiol. Plant 63: 309–314.Google Scholar
  9. Rout J.R. & N.P. Sarma, 1987. High frequency plantlet regeneration in rice anther culture. Rice Genet. Newslett. 3: 105–107.Google Scholar
  10. Rout J.R. & N.P. Sarma, 1990. Comparative morphological studies of microspore derived and F2 plants obtained from an interspecific rice hybrid (Oryza sativa Linn. x O. rufipogon Griff.). Plant Breeding 105: 283–291.Google Scholar
  11. Rout J.R., N.P. Sarma & G.J.N. Rao, 1989. Effect of Potato-2 medium on anther culture of interspecific rice hybrids. Ann. Bot. 63: 621–624.Google Scholar
  12. Wang C.C., C.S. Sun & Z. Chu, 1974. On the conditions for the induction of rice pollen plants and certain factors affecting the frequency of induction. Acta Bot. Sinica 16: 43–53 (In Chinese).Google Scholar
  13. Woo S.C. & I.J. Tung, 1972. Induction of rice plants from hybrid anthers of indica and japonica cross. Bot. Bull. Acad. Sin. 13: 67–70.Google Scholar
  14. Woo S.C. & C.Y. Huang, 1982. Anther culture of O. sativa L. x O. spontaneous Taiwania hybrids. Bot. Bull. Acad. Sin. 23: 39–44.Google Scholar
  15. Woo S.C., T. Mok, J.Y. Huang, 1978. Anther culture of O. sativa L. and O. perennis Moench Bot. Bull. Acad. Sin. 19: 171–178.Google Scholar
  16. Woo S.C., S.W. Ko & C.K. Wong, 1983. Anther culture of pollen plants derived from cross O. sativa L. x O. glaberrima Steud. Bot. Bull. Acad. Sin. 24: 53–58.Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • J. R. Rout
    • 1
  • N. P. Sarma
    • 2
  1. 1.Department of BotanyUniversity of CaliforniaDavisUSA
  2. 2.Directorate of Rice ResearchHyderabadIndia

Personalised recommendations