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A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants

Plant Molecular Biology volume 15, pages 921–932 (1990)Cite this article

Abstract

We previously reported that the expression of the wheat Cab-1 gene is regulated by an endogenous circadian rhythm and by the photoreceptor phytochrome both in wheat and in transgenic tobacco plants. To define regulatory elements necessary for the circadian rhythm-regulated Cab-1 gene expression, we now analysed the fluctuation of steady-state mRNA levels in a series of 5′ deletion mutants in transgenic tobacco plants. We found that the expression of a deletion mutant containing 211 bp upstream sequence still exhibited circadian rhythm. Furthermore we show that an enhancer-like sequence of the Cab-1 promoter (from −357 to −90) can endow a chimaeric gene consisting of a truncated 35S promoter (from −90 to +8) and the bacterial β-glucuronidase (GUS) gene with circadian clock-regulated gene expression. Finally we demonstrate by nuclear run-off experiments that the transcription rates of the Cab genes in wheat oscillate in a rhythmic manner, with a periodicity of approximately 24 hours. Consistent with our previous findings these results (i) indicate that the expression of the wheat Cab-1 gene is regulated mainly at the transcription level and (ii) identify a short promoter region between −211 and −90 that is responsible for the circadian clock-regulated gene expression.

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Affiliations

  1. Institute of Plant Physiology, Biological Research Center of the Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary

    Erzsebet Fejes, Aniko Pay, Ivan Kanevsky, Marta Szell, Eva Adam & Ferenc Nagy

  2. The Rockefeller University, 1230 York Avenue, 10021-6399, New York, NY, USA

    Steven Kay

Authors
  1. Erzsebet Fejes
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  2. Aniko Pay
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  3. Ivan Kanevsky
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  4. Marta Szell
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  5. Eva Adam
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  6. Steven Kay
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  7. Ferenc Nagy
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Fejes, E., Pay, A., Kanevsky, I. et al. A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants. Plant Mol Biol 15, 921–932 (1990). https://doi.org/10.1007/BF00039431

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  • DOI: https://doi.org/10.1007/BF00039431

Key words

  • circadian rhythm
  • transcription
  • transgenic plants
  • enhancer