Abstract
The culture of immature rice embryos is possible on a sterile medium made of 5 g of a mixture of mineral salts, 20 g dextrose and 7 g agar per liter water.
The test tubes should be sufficiently closed to prevent evaporation of water.
The quantity of endosperm adhering to the embryos has no influence on success of the culture.
It is possible to obtain seedlings from embryos of 7 days old, perhaps of 6 days. The germination is satisfactory for embryos which are 8 days old and it reaches 100% for embryos which are 10 days old at least.
The method can be applied to immature seeds derived from interspecific crosses and also to mature seeds which show a difficult germination.
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Now Chargé de Recherches of the FNRS (Fonds National de la Recherche Scientifique), Belgium
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Bouharmont, J. Embryo culture of rice on sterile medium. Euphytica 10, 283–293 (1961). https://doi.org/10.1007/BF00039098
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DOI: https://doi.org/10.1007/BF00039098