Abstract
Rhizobium leguminosarum strains that can form nodules on Pisum sativum cv. Afghanistan have been reported as uncommon in Europe, North America and Africa [11, 12]. The organization of the nodulation regions of the symbiotic plasmids of five strains of R. leguminosarum originating from Denmark [9], which can nodulate P. sativum cv. Afghanistan, was compared with that of a Turkish strain (TOM [18]) by DNA hybridizations. Four of the five Danish strains were found to be very similar to the Turkish strain with respect to the overall organizations of their respective nodulation regions.
References
Brewin NJ, Wood EA, Johnston AWB, Dibb NJ, Hombrecher G: Recombinant nodulation plasmids in Rhizobium leguminosarum. J Gen Microbiol 128: 1818–1827 (1982).
Brewin NJ, Beringer JE, Johnston AWB: Plasmid mediated transfer of host-range specificity between two strains of Rhizobium leguminosarum. J Gen Microbiol 120: 413–420 (1980).
Eckhardt T: A rapid method for the identification of plasmid DNA in bacteria. Plasmid 1: 584–588 (1978).
Gotz R, Evans JJ, Downie JA, Johnston AWB: Identification of the host-range DNA which allows Rhizobium leguminosarum strain TOM to nodulate cv. Afghanistan peas. Mol Gen Genet 201: 296–300 (1985).
Heath LS, Sloan GL, Heath HE: A simple and generally applicable procedure for releasing DNA from bacterial cells. Appl Environ Microbiol 51: 1138–1140 (1986).
Hobbs SLA, Delong CMO, Denes S, Iyer VN: Manipulation of nodulation specificity in the pea-Rhizobium leguminosarum symbiosis. In: Lugtenberg B (ed) Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions, pp 69–78. Springer-Verlag Press, Heidelberg (1986).
Holl FB: Host plant control of the inheritance of dinitrogen fixation in the Pisum-Rhizobium symbiosis. Euphytica 24: 767–770 (1975).
Hombrecher G, Gotz R, Dibb NJ, Downie JA, Johnston AWB, Brewin NJ: Cloning and mutagenesis of nodulation genes from Rhizobium leguminosarum TOM, a strain with extended host range. Mol Gen Genet 194: 293–298 (1984).
Jensen ES, Sørensen LH, Engvild KC: Danish Rhizobium leguminosarum strains nodulating “Afghanistan” pea (Pisum sativum). Physiol Plant 66: 46–48 (1986).
Johnston AWB, Beynon JL, Buchanan-Wollaston AV, Setchell SM, Hirsch PR, Beringer JE: High frequency transfer of nodulating ability between strains and species of Rhizobium. Nature 276: 634–636 (1978).
Kneen BE, LaRue TA: Peas (Pisum sativum L.) with strain specificity for Rhizobium leguminosarum. Heredity 52: 383–389 (1984).
Lie TA: Symbiotic specialization in pea plants: The requirement of specific Rhizobium strains for peas from Afghanistan. Ann Appl Biol 88: 462–465 (1978).
Maniatis T, Fritsch EF, Sambrook J: In: Molecular Cloning — a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1982).
Messing J: New M13 vectors for cloning. Methods Enzymol 101: 20–79 (1983).
Selvaraj G, Hooper I, Shantharam S, Iyer VN, Barran L, Wheatcroft R, Watson RJ: Derivation and molecular characterization of symbiotically deficient mutants of Rhizobium meliloti. Can J Microbiol 33: 739–747 (1987).
Soberon X, Covarrubias L, Bolivar F: Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene 9: 287–305 (1980).
Spaink HP, Okker RJH, Wijffelman CA, Pees E, Lugtenberg B: Promoters and operon structure of the nodulation region of the Rhizobium leguminosarum symbiosis plasmid pRL1JI. In: Lugtenberg B (ed) Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions, pp 55–68. Springer-Verlag, Heidelberg (1986).
Winarno R, Lie TA: Competition between Rhizobium strains in nodule formation: interaction between nodulating and non-nodulating strains. Plant Soil 51: 135–142 (1979).
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Nash, J.H.E., Ma, S. & Iyer, V.N. Characterization of Sym plasmids of Rhizobium leguminosarum strains able to nodulate Pisum sativum cv Afghanistan. Plant Mol Biol 11, 427–432 (1988). https://doi.org/10.1007/BF00039023
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DOI: https://doi.org/10.1007/BF00039023