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Improvement of legumes by exploring extra-specific genetic variation

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Summary

Genetic improvement of legumes is a major on-going challenge for plant breeders. Outlined in this review are the main reasons why such increased extra-specific genetic variation is required, particularly in the developing world. With the development of new methods of plant genetic manipulation it is necessary to assess the relative merits of all approaches now available. To help the plant breeder in this respect we have in this review comprehensively described these conventional and unconventional procedures with particular reference to economically important legumes.

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References

  • Al-Yasiri, S. A. & D. P. Coyne, 1966. Interspecific hybridisation in the genus Phaseolus. Crop Sci. 6: 59–61.

    Google Scholar 

  • Amirsahi, M. C. & Tavakoli, 1970. Protein content of five different species of pulse crops. In: Improving plant proteins by nuclear techniques. IAEA, Vienna: 331–335.

    Google Scholar 

  • Armstrong, J. M., 1954. Cytological studies in alfalfa polyploids. Can. J. Bot. 32: 531–542.

    Google Scholar 

  • Arnold, S. V. & T. Erikson, 1976. Factors influencing the growth and division of pea mesophyll protoplasts. Physiol. Plant. 36: 193–196.

    Google Scholar 

  • Barnett, O. W., P. B. Gibson & A. Seo, 1975. A comparison of heat treatment and meristem tip-culture for obtaining virus free plants of Trifolium repens. Pl. Dis. Reptr. 59: 834–837.

    Google Scholar 

  • Bent, F. C., 1962. Interspecific hybridisation in the genus Lotus. Can. J. Genet. Cytol. 4: 151–159.

    Google Scholar 

  • Bharal, S. R. & A. Rahid, 1979. Regeneration of plants from tissue cultures of the legume Indigofera enneaphylla L. Z. Pflanzenphysiol. 92: 443–448.

    Google Scholar 

  • Bhojwani, S. S., P. K. Evans & E. C. Cocking, 1977. Protoplast technology in relation to crop plants: Progress and Problems. Euphytica 26: 343–360.

    Google Scholar 

  • Binding, H. & R. Nehls, 1978a. Regeneration of isolated protoplasts of Vicia faba L. Z. Pflanzenphysiol. 88: 327–332.

    Google Scholar 

  • Binding, H. & R. Nehls, 1978b. Somatic cell hybridisation of Vicia faba plus Petunia hybrida. Molec, gen. Gebet. 164: 137–144.

    Google Scholar 

  • Bingham, E. T., 1968. Genetics and cytology of an albino in a diploid hybrid of M. sativa and M. falcata. Can. J. Genet. Cytol. 10: 357–361.

    Google Scholar 

  • Bingham, E. T. & T. J. McCoy, 1979. Cultivated alfalfa at the diploid level: origin, reproductive stability, and yield of seed and forage. Crop Sci. 19: 97–100.

    Google Scholar 

  • Bingham, E. T., L. V. Hurley, D. M. Kaatz & J. W. Saunders, 1975. Breeding alfalfa which regenerates from callus tissue in culture. Crop. Sci. 15: 719–721.

    Google Scholar 

  • Blaydes, D. F., 1966. Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant. 19: 748–753.

    Google Scholar 

  • Bliss, F. A. & T. C. Hall, 1977. Food legumes-compositional and nutritional changes induced by breeding. Cereal Fds. Wld. 22: 106–113.

    Google Scholar 

  • Bond, D. A., 1970. Yield and components of yield in diallel crosses between inbred lines of winter beans (Vicia faba). J. Agric. Sci. 67: 325–336.

    Google Scholar 

  • Braak, J. P. & E. Kooistra, 1975. A successful cross between Phaseolus vulgaris L. and Phaseolus ritensis Jones with the aid of embryo culture, Euphytica 24: 669–679.

    Google Scholar 

  • Brim, C. A., 1973. Quantitative genetics and breeding. In: B. E. Caldwell (Ed.), Soybeans: improvement, production and uses. Am. Soc. Agron. Inc. Madison: 155–186.

    Google Scholar 

  • Cionini, P. G., A. Bennica & F. d'Amato, 1978. Nuclear cytology of callus induction development in vitro Part I. Callus from V. faba cotyledons. Protoplasma 96: 101–112.

    Google Scholar 

  • Clement, W. M., 1964. Stability of chromosome numbers in tissue cultures of alfalfa, Medicago sativa L. Amer. J. Bot. 51: 70 (Suppl.).

    Google Scholar 

  • Cleveland, R. W. & E. H. Stanford, 1959. Chromosome pairing in hybrids between tetraploid Medicago sativa L. and diploid Medicago falcata L. Agron. J. Bot. 51: 488–492.

    Google Scholar 

  • Cobley, L. S. & W. M. Steele, 1976. An introduction to the botany of tropical crops, Longman, London.

    Google Scholar 

  • Cocking, E. C., 1972. Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23: 29–50.

    Article  Google Scholar 

  • Cocking, E. C., 1981. Opportunities from the use of protoplasts Royal Society Meeting on the manipulation of genetic systems in plant breeding, October, 1980. Phil. Trans. R. Soc. B 292: 557–568.

    Google Scholar 

  • Cocking, E. C. & J. F. Peberdy, 1974. The use of protoplasts from fungi and higher plants as gametic systems — A practical handbook, Botany Dept., Univ. Nottingham, U.K.: 67.

    Google Scholar 

  • Cocking, E. C. & R. Riley, 1979. Application of the tissue culture and somatic hybridisation to plant improvement. Plant Breeding Symposium II, Iowa State Univ., U.S.A.

  • Constabel, F., D. Dudits, O. L. Gamborg & K. N. Kao, 1975. Nuclear fusion in intergeneri heterokaryons. A note. Can. J. Bot: 53: 2092–2095.

    Google Scholar 

  • Constabel, F., G. Webber & J. W. Kirkpatrick, 1977. Sur la compatabilite des chromosomes dans les hybrides intergeneriques de cellules de Glycine max × Vicia hajastana. Histophysiologie Vegetale. C.R. Acad. Sci. Paris 285: 319–321.

    Google Scholar 

  • Constabel, F., G. Webber, J. W. Kirkpatrick & Pahl, 1976. Cell division of intergeneric protoplast fusion products. Z. Pflanzenphysiol. 79: 1–7.

    Google Scholar 

  • Constabel, F., J. W. Kirkpatrick & O. L. Gamborg, 1973. Callus formation from mesophyll protoplasts of Pisum sativum. Can. J. Bot. 51: 2105–2106.

    Google Scholar 

  • Coyne, D. P. & M. L. Schuster, 1975. Genetic and breeding strategy for resistance to rust (Uromyces phaseoli Reben) Wint in beans (Phaseolus vulgaris L.). Euphytica 24: 795–803.

    Google Scholar 

  • Crocomo, O. J., N. A. Tulman, S. Blixit & K. Mikaelsen, 1976. Breeding for protein in the bean (Phaseolus vulgaris L.). Inventory of some Brazilian varieties and a number of lines of different origin. In: Evolution of seed protein alterations by mutation breeding. IAEA, Vienna: 197–208.

    Google Scholar 

  • Davey, M. R., E. Bush & J. B. Power, 1974. Cultural studies of a dividing legume protoplast system. Pl. Sci Letters 3: 127–133.

    Google Scholar 

  • Delwiche, C. C., 1978. Legumes-past, present and future. Bioscience 28: 565–570.

    Google Scholar 

  • Deodika, G. B. & C. V. Thakar, 1956. Cytotaxonomic evidence for the affinity between Cajanus indicus Spreng and certain erect species of Atylosia W. & A. Proc. Indian Acad. Sci. 43: 37–45.

    Google Scholar 

  • Dhaliwal, A. S., L. H. Pollard & A. P. Lorez, 1962. Cytological behaviour of an F1 species cross (Phaseolus lunatus L. var. Fordhood × Phaseolus polystachys L.). Cytologia 27: 369–374.

    Google Scholar 

  • Donn, G., 1978. Cell division and callus regeneration from leaf protoplasts of Vicia narbonensis. Z. Pflanzenphysiol. 86: 65–75.

    Google Scholar 

  • Ellerstörm, S. & J. Sjödin, 1966. Frequency and vitality of aneuploids in a population of tetraploid red clover. Heredita 55: 166–182.

    Google Scholar 

  • Empig, L. T. & W. R. Fehr, 1971. Evaluation of methods for generation advance in bulk hybrid soybean population. Crop Sci. 11: 51–54.

    Google Scholar 

  • Evans, A. M. & H. E. Girdley, 1979. Prospects for the improvement of protein and yield in food legumes. Curr. Advn. Plt. Sci. 11: 32. 11–17.

    Google Scholar 

  • Fam, B. F., 1969. Overcoming incompatibility in the remote hybridisation of pea with bean by irradiating pollen of the parental form. (Ru.). Sb. rabot molodykh. uchenickh. Vses. Selekts.-genet. in-t 69–71.

  • Fowke, L. C., P. J. Rennie, J. W. Kirkpatrick & F. Constabel, 1976. Ultrastructure of fusion products of soybean culture and sweet clover leaf protoplasts. Planta 130: 39–45.

    Google Scholar 

  • Fozdar, B. S., 1963. Cytological investigations of parents, offsprings, and back cross derivatives involved in the interspecific cross Phaseolus lunatus L. × P. polystachys(L.). BSP. Diss. Abstr. 24: 480–1.

    Google Scholar 

  • Gamborg, O. L., 1966. Aromatic metabolism in plants II. Enzymes of the shikimate pathway in suspension cultures of plant cells. Can. J. Biochem. 44: 791–799.

    PubMed  Google Scholar 

  • Gamborg, O. L., F. Constabel & J. P. Shyluk, 1974. Organogenesis in callus from shoot apices of Pisum sativum. Physiol. Plant 30: 125–128.

    Google Scholar 

  • Gamborg, O. L., J. Shyluk & K. K. Kartha, 1975. Factors affecting the isolation and callus formation in protoplasts from the shoot apices of Pisum sativum L. Pl. Sci. Letters 4: 285–292.

    Article  Google Scholar 

  • Gamborg, O. L., R. A. Miller & K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158.

    PubMed  Google Scholar 

  • Gillies, C. B., 1970. Alfalfa chromosomes I. Pachytene karyotype of a diploid Medicago falcata L. and its relationship to M. sativa L. Crop Sci. 10: 169–171.

    Google Scholar 

  • Graham, P. H., 1968. Growth of Medicago sativa and Trifolium subterraneum L. in callus and suspension cultures. Phyton 25: 159–162.

    Google Scholar 

  • Grant, M. E. & K. W. Fuller, 1968. Tissue culture of root cells of Vicia faba. J. Exp. Bot. 19: 667–680.

    Google Scholar 

  • Grant, W. F., M. R. Bullen & D.de Nettancourt, 1962. The cytogenetics of Lotus I. Embryo cultured interspecific diploid hybrids closely related to L. corniculatus L. J. Exp. Bot. 4: 105–128.

    Google Scholar 

  • Gregory, W. C., 1966. Mutation breeding. In: K. J. Frey (Ed.), Plant breeding, University Press, Ames: 189–218.

    Google Scholar 

  • Green, A. G., R. W. Oram & B. J. Read, 1977. Genetic variation for seed yield, protein content, oil content and seed weight in Lupinus albus. Aust. J. Agric. Res., 785–793.

  • Gresshoff, P. M., 1980. In vitro culture of white clover: Callus, suspension, protoplast culture, and plant regeneration. Bot. Gaz. 141: 157–164.

    Article  Google Scholar 

  • Gamblin, J., 1977. Plant breeding interpretations of the effect of bulk breeding on four populations of beans (Phaseolus vulgaris L.). Euphytica 26: 157–168.

    Google Scholar 

  • Ho, K. M. & K. J. Kasha, 1972. Chromosome homology at pachytene in diploid Medacago sativa, M. falcata and their hybrids. Can. J. Genet. Cytol. 14: 829–838.

    Google Scholar 

  • Honma, S., 1956. A bean interspecific hybrid. J. Hered. 47: 217–220.

    Google Scholar 

  • Honma, S. & O. Heckett, 1959. Interspecific between Phaseolus vulgaris × P. lunatus. J. Hered. 50: 233–237.

    Google Scholar 

  • Jain, H. K., 1973. Genetic improvement and production prospects of food legumes. In: Tropical Agricultural Series No. 6, Ministry of Agry. and Forestry, Govt. of India: 33–42.

  • Kao, K. N., 1977. Chromosomal behaviour in somatic hybrids of soybean Nicotiana glauca. Molec. gen. Genet. 150: 225–230.

    Article  Google Scholar 

  • Kao, K. N. & M. R. Michayluk, 1974. A method for high frequency intergeneric fusion of plant protoplasts. Planta 115: 355–367.

    Google Scholar 

  • Kao, K. N. & M. R. Michayluk, 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110.

    Google Scholar 

  • Kao, K. N. & M. R. Michayluk, 1980. Plant regeneration from mesophyll protoplasts of alfalfa. Z. Pflanzenphysiol. 96: 135–142.

    Google Scholar 

  • Kao, K. N., F. Constabel, M. R. Michayluk & O. L. Gamborg, 1974. Plant protoplast fusion and growth of intergenric hybrid cells. Planta 120: 215–227.

    Google Scholar 

  • Kao, K. N., O. L. Gamborg, R. A. Miller & W. Keller, 1971. Cell divisions in cells regenerated from protoplasts of soybean and Haplopappus gracilis. Nature 232: 124.

    PubMed  Google Scholar 

  • Kao, K. N., W. A. Keller & R. A. Miller, 1970. Cell division in newly formed cells from protoplasts of soybean, Exp. Cell. Res. 62: 338–340.

    PubMed  Google Scholar 

  • Kartha, K. K., O. L. Gamborg & F. Constabel, 1974a. Regeneration of pea (Pisum sativum L.) plant from shoot apical meristem. Z. Pflanzenphysiol. 72: 172–176.

    Google Scholar 

  • Kartha, K. K., O. L. Gamborg, F. Constabel & K. N. Kao, 1974b. Fusion of rapeseed and soybean protoplasts and subsequent division of heterokaryocytes. Can. J. Bot. 52: 2435–2436.

    Google Scholar 

  • Keller, W. A. & G. Melchers, 1973. Effect of high pH and calcium on tobacco leaf protoplasts fusion. Z. Naturforsch. 28: 737–741.

    Google Scholar 

  • Kelley, J. F., 1975. Increasing protein quantity and quality. In: M. Milner (Ed.), Nutritional improvement of food legumes by breeding. PAG Symposium, FAO, Rome. Wiley, New York: 174–184.

    Google Scholar 

  • King, P. J., I. Potrykus & E. Thomas, 1978. In vitro genetics of cereals: problems and perspectives. Physiol. Veg. 16: 381–399.

    Google Scholar 

  • Knobloch, I. W., 1972. Intergeneric hybridisation of flowering plants. Taxon 21: 97–104.

    Google Scholar 

  • Kumar, L. S. S., M. V. Thombre & R. d'Cruz, 1958. Cytological studies of an intergeneric hybrid of Cajanus caja (L.) Millisp. and Atylosia lineata W. & A. Proc. Ind. Acad. Sci. 40: 3–10.

    Google Scholar 

  • Lamprecht, H., 1959. Der Artbegriff. Seine Entwicklung und experimentelle Klarlegung. Agri. Hort. Genet. 17: 103–264.

    Google Scholar 

  • Landgren, C. R. & J. G. Torrey, 1973. The culture of protoplasts derived from explants of seedling pea shoots. Colloq. Int. Cent. Res. Sci. 212: 281.

    Google Scholar 

  • Lautour, G.de, W. T. Jones & M. D. Ross, 1978. Production of interspecific hybrids in Lotus aided by endosperm transplants. New Zealand J. Bot. 16: 61–68.

    Google Scholar 

  • Lesins, K., 1957. Cytogenetic study on a tetraploid plant at the diploid chromosome level. Can. J. Bot. 35: 181–196.

    Google Scholar 

  • Liau, D. F. & W. G. Boll, 1970. Callus and cell suspension culture of bush bean (Phaseolus vulgaris). Can. J. Bot. 48: 1119–11330.

    Google Scholar 

  • McComb, J. A., 1975. Is intergeneric hybridisation in the leguminosae possible? Euphytica 24: 497–502.

    Google Scholar 

  • McCoy, T. J. & E. T. Bingham, 1977. Regeneration of diploid alfalfa plants from callus grown in suspension cultures. Pl. Sci. Letters 10: 59–66.

    Article  Google Scholar 

  • Mehta, A. R., G. G. Henshaw & H. E. Street, 1967. Aspects of growth in suspension cultures of Phaseolus vulgaris L. and Linum usitatissimum L. Indian J. Pl. Physiol. 10: 44–53.

    Google Scholar 

  • Mehta, R. K., K. N. Subramanyam & M. S. Swaminathan, 1965. Studies on induced polyploids on forage crops V. Studies in Senji. Ind. J. Genet. 25: 305–316.

    Google Scholar 

  • Mendel, G. J. 1866. Versuche über Pflanzenhybriden. Verh. Naturf. Vereins Brunn 4: 3–47.

    Google Scholar 

  • Milner, M. (Ed.), 1976. Symbiotic nitrogen fixation in plants. Cambridge University Press, Cambridge.

    Google Scholar 

  • Mokhtarazadeh, A. & M. J. Constantin, 1978. Plant regeration from hypocotyl and anther derived callus of Berseen clover. Crop Sci. 18: 567–572.

    Google Scholar 

  • Mukhopadhyay, A. & S. S. Bhojwani, 1978. Shoot bud differentiation in tissue cultures of leguminous plants. Z. Pflanzenphysiol. 88: 263–268.

    Google Scholar 

  • Murashige, T., 1978. The impact of plant tissue culture on agriculture, IAPTC publication, Calgary: 15–26.

    Google Scholar 

  • Niizeki, M. & F. Kita, 1973. Studies on plant cell and tissue culture III. In vitro induction of callus from anthers of forage crops. J. Facult. Agric. Hokkaido Univ. (Psappora) Japan 57: 293–300.

    Google Scholar 

  • Niizeki, M. & W. F. Grant, 1971. Callus plantlet formation and polyploidy from cultured anthers of Lotus and Nicotiana. Can. J. Bot. 49: 2041–2051.

    Google Scholar 

  • Norman, A. G., 1978. Soybean, physiology, agronomy and utilization. Academic Press, London.

    Google Scholar 

  • Ojoma, O. A., 1974. Yield potential of cow peas Vigna unguiculata (L.) Walp: results of mass and bulk pedigree selection methods in Western Nigeria. Nigerian Agric. J. 11: 150–156.

    Google Scholar 

  • Oswald, T. H., A. E. Smith & D. V. Phillips, 1077. Callus and plantlet regeneration from cell cultures of ladino clover and soybean. Physiol. Plant. 39: 129–134.

    Google Scholar 

  • Pandey, S. & E. T. Gritton, 1976. Observed and predicted response to selection and yield in peas. Crop Sci. 16: 289–292.

    Google Scholar 

  • Papa, K. E., J. H. Williams & O. G. Hanway, 1961. Effectiveness of selection for quantitative characters in the third generation following irradiation of soybean seeds with x-ray and thermal neutrons. Crop Sci. 1: 87–90.

    Google Scholar 

  • Patil, S. H., 1970. Trombay ground nut selection for increased oil content and yield. Indian J, Agric. Sci. 43: 370–376.

    Google Scholar 

  • Pelcher, L. E., O. L. Gamborg & K. N. Kao, 1974. Bean mesophyll protoplasts production, culture and callus formation. Pl. Sci. Letters 3: 107–111.

    Google Scholar 

  • Pelletier, G. & A. Pelletier, 1971. Culture in vitro de tissues de trèfle blanc (Trifolium repens): variabilité des plantes régénérées. Ann. Amélior. Plantes 21: 221–233.

    Google Scholar 

  • Phillips, G. C. & G. B. Collins, 1978. In vitro tissue culture and plant regeneration from callus cultures of red clover (T. pratense). Crop Sci. 19: 59–64.

    Google Scholar 

  • Power, J. B., S. E. Cummins & E. C. Cocking, 1970. Fusion of isolated plant protoplasts. Nature 225: 1016–1018.

    Google Scholar 

  • Ramawat, K. G., R. RajBhansal & H. C. Arya, 1977. Differentiation in Crotalaria callus cultures. Phytomorphology 27: 303–307.

    Google Scholar 

  • Razdan, M. K., E. C. Cocking & J. B. Power, 1980. Callus regeneration from mesophyll protoplasts of sweet pea (Lathyrus odoratus L.). Z. Pflanzenphysiol. 96: 181–184.

    Google Scholar 

  • Roy, A. & D. N. De, 1965. Intergeneric hybridisation of Cajanus and Atylosia. Sci. & Cult. 31: 93–95.

    Google Scholar 

  • Santos, A. V. P.dos, D. E. Outka, E. C. Cocking & M. R. Davey, 1980. Organogenesis and somatic embryogenesis in tissues derived from leaf protoplasts and leaf explants of Medicago sativa. Z. Pflanzenphysiol. 99: 261–270.

    Google Scholar 

  • Saunders, J. W. & E. T. Bingham, 1975. Growth regulator effects on bud initiation in callus cultures of Medicago sativa. Amer. J. Bot. 62: 850–855.

    Google Scholar 

  • Schenk, R. U. & A. C. Hildebrandt, 1972. Medium and techniques for induction and growth of monocoty-ledons and dicotyledons cell cultures. Can. J. Bot. 50: 199–204.

    Google Scholar 

  • Scowcroft, W. R., 1980. Tissue culture research: current status and future potential. Rice Culture Planning Conference, International Rice Research Institute, Philippines, April 28–30.

  • Scowcroft, W. R. & J. A. Adamson, 1976. Organogenesis from callus cultures of legumes Stylosanthes hamata. Pl. Sci. Letters 7: 39–42.

    Article  Google Scholar 

  • Shamarao, H. K. & Narayanswamy, 1975. Effect of gamma irradiation and cell proliferation and regeneration in explanted tissues of pigeon pea, Cajanus cajan (L) Mills P. Radiation Botany 15: 301–303.

    Google Scholar 

  • Shannon, J. G., J. R. Wilcox & H. H. Probst, 1972. Estimated gains from selection for protein and yield in the F4 generation of six soybean populations. Crop Sci. 12: 824–826.

    Google Scholar 

  • Shepard, J. F., D. Bidney & E. Shahin, 1980. Potato protoplasts in crop improvement. Crop Sci. 12: 824–826.

    Google Scholar 

  • Shepard, J. F., D. Bidney & E. Shahin, 1980. Potato protoplasts in crop improvement. Science 28: 17–24.

    Google Scholar 

  • Singh, A. & R. P. Roy, 1971. Studies on the colchiploids of four species of Trigonella. Cytologia 36: 133–142.

    Google Scholar 

  • Smartt, J., 1970. Interspecific hybridisation between cultivated American species of the genus Phaseolus. Euphytica 19: 480–489.

    Google Scholar 

  • Smartt, J., 1976. Tropical pulses. Longman, London.

    Google Scholar 

  • Smartt, J. & N. Han, 1972. Fertility and segregation of the amphidiploid Phaseolus vulgaris. × P. coccineus L. and its behaviour in back crosses. Euphytica 21: 496–501.

    Google Scholar 

  • Sobolev, N. A., E. D. Zhila & G. K. Adamchuk, 1968. intergeneric hybridisation in the Viciae tribe (Ru.). Tastenievodstro, Selektsiya imlesovodstro No. 1, Moscow, USSR, Kolos, 86–94.

    Google Scholar 

  • Sobolev, N. A., G. K. Adamchuk & E. D. Zhila, 1970. Apomixis in peas (Ru.). Apomiksis. i. selektsiya, Moscow, USSR, Nauka 213–220.

    Google Scholar 

  • Somaroo, B. H. & W. F. Grant, 1971. Interspecific hybridisation between diploid species of Lotus (Leguminosae). Genetica 42: 353–367.

    Google Scholar 

  • Sprague, E. W., 1959. Cytological and fertility relationships of Medicago sativa, M. falcata and M. gaetula. Agron. J. 51: 249–252.

    Google Scholar 

  • Swanson, E. B. & D. T. Tomas, 1980. Plant regeneration from cell cultures of Lotus corniculatus and the selection and characterisation of 2,4-D tolerant cell lines. Can. J. Bot. 58: 1205.

    Google Scholar 

  • Taira, T., F. A. Haskins & H. J. Gorz, 1977. Callus and suspension cultures of Melilotus alba tissue and cells. Crop Sci. 17: 407–411.

    Google Scholar 

  • Torrey, J. G., 1967. Morphogenesis in relation to chromosomal constitution of long term tissue cultures. Physiol. Plant. 20: 265–275.

    Google Scholar 

  • Venkateswaran, S., 1962. Tissue culture studies on Vicia faba L. I. Establishment of cultures. Phytomorphology 12: 300–306.

    Google Scholar 

  • Venkateswaran, S. & O. Huhitinen, 1978. In vitro root and shoot differentiation from callus of a legume the winged bean Psophocarpus tetragonolobus. In Vitro 14: 355.

    Google Scholar 

  • Walker, K. A., Poly, C. Yu, Shirley, J. Bato & E. G. Jaworski, 1978. The hormonal control of organ formation in callus of Medicago sativa L. cultured in vitro. Amer. J. Bot. 65: 654–659.

    Google Scholar 

  • Williams, E., 1978. A hybrid between Trifolium repens and T. ambiguum obtained with the aid of embryo culture. New Zealand J. Bot. 16: 499–506.

    Google Scholar 

  • Yarnell, S. H., 1965. Cytogenetics of vegetable crops IV. Legumes. Bot. Rev. 31: 247–330.

    Google Scholar 

  • Zhila, E. D., 1966. Use of γ-irradiation to overcome incompatibility between leguminous crops. (Ru.) Nauc. Tmd. Ves. Naux-issled. Inst. zernob. kult. (Sci Trans. All-Un. Sci-Inst. Pulse Crops) No. 1: 70–76.

  • Zieg, R. G. & D. E. Outka, 1980. The isolation, culture and callus formation of soybean pod protoplasts. Pl. Sci. Letters 18: 105–114.

    Google Scholar 

  • Zohary, D. & M. Hopf, 1973. Domestication of pulses in the old world. Science 182: 887–894.

    Google Scholar 

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Razdan, M.K., Cocking, E.C. Improvement of legumes by exploring extra-specific genetic variation. Euphytica 30, 819–833 (1981). https://doi.org/10.1007/BF00038811

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