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Morphogenesis in tobacco subepidermal cells: Putrescine as marker of root differentiation

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Abstract

We have used the tobacco thin cell layer ‘in vitro’ system to evaluate changes in polyamine titers as correlated with root differentiation and with variations in external pH during culture. We show that root differentiation in this system depends on both a rise in putrescine titers and a drop of pH, each of these two factors acting independently. With respect to polyamine titers, the most dramatic changes occur in the levels of putrescine liberated from perchloric acid-soluble conjugates. These titers increase from day 0 to day 7 of culture, reaching almost 2000 nmol g-1 fresh weight. Inhibition of putrescine biosynthesis prevents root initiation, while exogenous putrescine supply reverses this effect. We conclude that putrescine is a good marker for root differentiation.

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References

  1. Chatterjee S, Choudhuri MM, Ghosh B (1983) Changes in polyamine contents during root and nodule growth of Phaseolus mungo. Phytochemistry 22: 1553–1556

    Google Scholar 

  2. Chlyah H (1974) Intertissue correlations in organ fragments. Plant Physiol 54: 341–348

    Google Scholar 

  3. Chriqui D, D'Orazi D, Bagni N (1986) Ornithine and arginine decarboxylases and polyamine involvement during in vivo differentiation and in vitro dedifferentiation of Datura innoxia leaf explants. Physiol Plant 68: 589–596

    Google Scholar 

  4. Feirer RP, Mignon G, Litvay JD (1984) Arginine decarboxylase and polyamines required for embryogenesis in the wild carrot. Science 223: 1433–1435

    Google Scholar 

  5. Feirer RP, Wann SR, Einspahr DW (1985) The effects of spermidine synthesis inhibitors on in-vitro plant development. Plant Growth Reg 3: 319–327

    Google Scholar 

  6. Friedman R, Altman A, Bachrach U (1982) Polyamines and root formation in mung bean hypocotyl cuttings. Plant Physiol 70: 844–848

    Google Scholar 

  7. Friedman R, Altman A, Bachrach U (1985) Polyamines and root formation in mung bean hypocotyl cuttings: incorporation of precursors into polyamines. Plant Physiol 79: 80–83

    Google Scholar 

  8. Galston AW (1983) Polyamines as modulators of plant development. BioScience 6: 382–388

    Google Scholar 

  9. Jarvis BC, Shannon PRM, Yasmin S (1983) Involvement of polyamines with adventitious root development in stem cuttings of mung bean. Plant Cell Physiol 24: 677–683

    Google Scholar 

  10. Kallio A, McCann PP, Bey P (1981) DL-α-(difluoromethyl)-arginine: a potent enzyme-activated irreversible inhibitor of bacterial arginine decarboxylases. Biochemistry 20: 3163–3166

    Google Scholar 

  11. Kaur-Sawhney R, Tiburcio AF, Galston AW (1988) Spermidine and flower-bud differentiation in thin-layer explants of tobacco. Planta 173: 282–284

    Google Scholar 

  12. Malfatti H, Vallée JC, Perdrizet E, Carré M, Martin C (1983) Acides aminés et amines libres d'explants foliaires de Nicotiana tabacum cultivés in vitro sur des milieux induisant la rhizogenèse ou la caulogènese. Physiol Plant 57: 492–498

    Google Scholar 

  13. Malmberg RL, McIndoo J (1983) Abnormal floral development of a tobacco mutant with elevated polyamine levels. Nature 305: 623–625

    Google Scholar 

  14. Metcalf BW, Bey P, Danzin C, Jung MJ, Casara P, Vevert JP (1978) Catalytic irreversible inhibition of mammalian ornithine decarboxylase (EC 4.1.1.17) by substrate and product analogues. J Amer Chem Soc 100: 2551–2553

    Google Scholar 

  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  16. Mutafschiev S, Cousson A, Tran Thanh Van K (1987) Modulation of cell growth and differentiation by pH and oligosaccharides. In: Jackson MB, Mantell SH, Blake J (Eds) Advances in the Chemical Manipulation of Plant Tissue Culture (pp 29–42) British Plant Growth Regulator Group Monograph No 16

  17. Ramakrishna S, Adiga PR (1975) Arginine decarboxyase from Lathyrus sativus seedlings: purification and properties. Eur J Biochem 59: 377–386

    Google Scholar 

  18. Schwartz M, Altman A, Cohen Y, Arzee T (1986) Localization of ornithine decarboxylase and changes in root meristems of Zea mays. Physiol Plant 67: 485–492

    Google Scholar 

  19. Shyr Y-Y, Kao C-H (1985) Polyamines and root formation in mung bean hypocotyl cuttings. Bot Bull Academia Sinica 26: 179–184

    Google Scholar 

  20. Simola LK, Honkanen J (1983) Organogenesis and fine structure in megagametophyte callus lines of Picea abies. Physiol Plant 59: 551–561

    Google Scholar 

  21. Slocum RD, Kaur-Sawhney R, Galston AW (1984) The physiology and biochemistry of polyamines in plants. Arch Biochem Biophys 235: 283–303

    Google Scholar 

  22. Smith TA (1985) Polyamines. Ann Rev Plant Physiol 36: 117–143

    Google Scholar 

  23. Tiburcio AF, Kaur-Sawhney R, Ingersoll RB, Galston AW (1985) Correlation between polyamines and pyrrolidine alkaloids in developing tobacco callus. Plant Physiol 78: 323–326

    Google Scholar 

  24. Tiburcio AF, Kaur-Sawhney R, Galston AW (1987) Polyamines and organogenesis in thin layer tobacco tissue cultures. In: Jackson MB, Mantell SH, Blake J (Eds) Advances in the Chemical Manipulation of Plant Tissue Culture (pp 43–56) British Plant Growth Regulator Group Monograph No 16

  25. Tiburcio AF, Kaur-Sawhney R, Galston AW (1987) Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures. Plant Cell Tissue Organ Culture 9: 111–120

    Google Scholar 

  26. Tiburcio AF, Kaur-Sawhney R, Galston AW (1988) Polyamine biosynthesis during vegetative and floral bud differentiation in thin layer tobacco tissue cultures. Plant Cell Physiol 29: 1241–1249

    Google Scholar 

  27. Torrigiani P, Altamura MM, Pasqua G, Monacelli B, Serafini-Fracassini D, Bagni N (1987) Free and conjugated polyamines during de novo floral and vegetative bud formation in thin cell layers of tobacco. Physiol Plant 70: 453–460

    Google Scholar 

  28. Tran Thanh Van K (1973) Direct flower neoformation from superficial tissues of small explant of Nicotiana tabacum L. Planta 115: 87–92

    Google Scholar 

  29. Tran Thanh Van K, Dien NT (1975) Etude au niveau cellulaire de la différentiation in vitro et de novo de burgeons végétatifs, de racines ou de cal à partir de couches minces de cellules de type épidermique de Nicotiana tabacum Wisc. 38. Can J Bot 53: 553–559

    Google Scholar 

  30. Tran Thanh Van K, Toubart P, Cousson A, Darvill AG, Gollin DJ, Chelf P, Albersheim P (1985) Manipulation of the morphogenetic pathways of tobacco explants by oligosaccharins. Nature 314: 615–617

    Google Scholar 

  31. Van Overbeek J, Gordon S, Gregory L (1946) Analysis of the function of the leaf in the process of root formation in cuttings. Amer J Bot 33: 100–107

    Google Scholar 

  32. Wang SY, Faust M (1986) Effect of growth retardants on root formation and polyamine content in apple seedlings. J Amer Soc Hort Sci 111: 912–917

    Google Scholar 

  33. Young ND, Galston AW (1983) Putrescine and acid stress. Plant Physiol 71: 767–771

    Google Scholar 

  34. Young ND, Galston AW (1985) Control of arginine decarboxylase biosynthesis in stressed oat leaf segments. In: Selmeci L, Brosnan ME, Seiler N (Eds) Recent Progress in Polyamine Research (pp 527–535)

  35. Zivy M, Cousson A, Thiellement H, Tran Thanh Van K (1987) Etude de la différentiation in vitro d'explants de tabac par électrophorèse bidimensionnelle de protéines. Revue de l'Institut Pasteur de Lyon 20: 283–285

    Google Scholar 

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Authors and Affiliations

  1. Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, 08028, Barcelona, Spain

    Antonio F. Tiburcio

  2. Institut de Physiologie Végétale, CNRS, 91190, Gif sur Yvette Cedex, France

    Cyrille Amin Gendy & Kiem Tran Thanh Van

Authors
  1. Antonio F. Tiburcio
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  2. Cyrille Amin Gendy
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  3. Kiem Tran Thanh Van
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Tiburcio, A.F., Gendy, C.A. & Tran Thanh Van, K. Morphogenesis in tobacco subepidermal cells: Putrescine as marker of root differentiation. Plant Cell Tiss Organ Cult 19, 43–54 (1989). https://doi.org/10.1007/BF00037775

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  • DOI: https://doi.org/10.1007/BF00037775

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