Abstract
Leaves were obtained from 4-week-old seedlings of Lavandula latifolia Medicus grown in vitro. Leaf explants were then cultured on MS medium supplemented with different concentrations and combinations of the auxins IAA or NAA with the cytokinin BA and maintained under three illumination conditions, 16h photoperiod, darkness or darkness followed by a photoperiod, to assess morphogenic responses. Irrespective of illumination conditions, bud regeneration was achieved only in media containing BA or BA/auxin combinations, with the best results being obtained in the presence of BA and 0.06 or 0.6 μM IAA or NAA. A photoperiod of 16h appeared to yield the best response in terms of bud regeneration percentage. High auxin concentrations (6.0 or 11.0 μM) inhibited bud differentiation, especially when explants were cultured in darkness. On the other hand, low auxin levels and photoperiod improved shoot development. Excised shoots were induced to form roots by transfer to hormone-free MS medium with macronutrients at half strength. The obtained plantlets were ultimately grown in the greenhouse.
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Abbreviations
- BA:
-
benzyladenine
- BM:
-
basal medium
- IAA:
-
indoleacetic acid
- MS:
-
Murashige & skoog
- NAA:
-
α-naphthaleneacetic acid
References
Calvo MC, Segura J (1988) In vitro morphogenesis from explants of Lavandula latifolia and Lavandula stoechas seedlings. Scientia Hortic 36: 131–137
Calvo MC, Jordan A, Segura J (1988) Plant regeneration from isolated cells of Lavandula latifolia Medicus. In Vitro 24: 943–946
Evans ML (1984) Functions of hormones at the cellular level of organization. In: Scott TK (Ed) Hormonal Regulation of Development II (pp 23–79) Encyclopedia of Plant Physiology, New Series, Vol 10. Springer-Verlag, Berlin
Font-Quer P (1978) Plantas Medicinales. Labor Press, Barcelona
Horsch R, Fraley R, Rogers S, Fry J, Klee H, Shah D, McCormick S, Niedermeyer J, Hoffmann N (1987) Agrobacterium-mediated transformation of plants. In: Green CE, Somers DA, Hackett WP, Biesboer DD (Eds) Plant Tissue and Cell Culture (pp 317–329) Alan R Liss, New York
Horsch R, Fry JE, Hoffmann N, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231
Klee H, Horsch R, Rogers S (1987) Agrobacterium-mediated plant transformation and its further applications to plant biology. Ann Rev Plant Physiol 38: 467–486
McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Leaf disc transformation of cultivated tomato (L. esculetum) using Agrobacterium tumefaciens. Plant Cell Rep 5: 81–84
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497
O'Brien T, Beall FD, Smith H (1985) De-etiolation and plant hormones. In: Pharis RP, Reid DM (Eds) Hormonal Regulation of Development III (pp 282–307) Encyclopedia of Plant Physiology, New Series, Vol 11. Springer-Verlag, Berlin
Rajagopal R, Ulvskov P, Marcussen J (1986) Effects of light on endogenous hormone levels in plants. In: Purohit SS (Ed) Hormonal Regulation of Plant Growth and Development, Vol 3 (pp 29–90) AgroBotanical Publishers, India
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Calvo, M.C., Segura, J. Plant regeneration from cultured leaves of Lavandula latifolia Medicus: Influence of growth regulators and illumination conditions. Plant Cell Tiss Organ Cult 19, 33–42 (1989). https://doi.org/10.1007/BF00037774
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DOI: https://doi.org/10.1007/BF00037774