Skip to main content
Log in

Plant regeneration from cotyledons of Prunus persica, Prunus domestica, and Prunus cerasus

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript


Shoots were regenerated from the proximal region of immature cotyledons (with the embryonic axis removed) of Prunus persica (peach) and from the same area in mature cotyledons of P. domestica (plum) and P. cerasus (sour cherry) on MS medium containing (in mgl-1) thiamine-HCl, 0.4; nicotinic acid, 0.5; pyridoxine-HCl, 0.5; sucrose, 25 000; and 0.7% agar. The medium was supplemented with 0.0–2.5 μM indole-butyric acid and 5–12.5 μM thidiazuron. Cultures were incubated at 24 °C under 16h photoperiod. Shoots regenerated adventitiously over a broad range of thidiazuron concentrations and 2.5 μM indole-butyric acid in 35 days. The presence of the embryonic axis inhibited the development of shoots. Regenerated shoots of peach and plum were rooted on half-strength MS inorganic semi-solid medium with 2.5–5.0 μM indole-butyric acid. Rooted plants were acclimatized and transferred to the greenhouse.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others





indole-butyric acid




indole-acetic acid


α-naphthalene-acetic acid


  1. Druart P (1980) Plant regeneration from root callus of different Prunus species. Scientia Hort 12: 339–342

    Google Scholar 

  2. Druart P (1981) Embryogenèse somatique et obtention de plantules chez Prunus incisa × serrula (GM9) cultivé in vitro. Bull Rech Agron Gembloux 16: 205–220

    Google Scholar 

  3. Fellman CD, Read PE, Hosier MA (1987) Effects of thidiazuron and CPPU on meristem formation and shoot proliferation. Hort Sci 22: 1197–1200

    Google Scholar 

  4. Hammerschlag FA, Bauchan G, Scorza R, (1985) Regeneration of peach plants from callus derived from immature embryos. Theor Appl Genet 70: 248–251

    Google Scholar 

  5. Lane WD, Cossio F (1986) Adventitious shoots from cotyledons of immature cherry and apricot embryos. Can J Plant Sci 66: 953–959

    Google Scholar 

  6. Mehra A, Mehra PN (1974) Organogenesis and plantlet formation in vitro in almond. Bot Gaz 135: 61–73

    Google Scholar 

  7. Mok MC, Mok DWS, Turner JE, Mujer CV (1987) Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems. Hort Sci 22: 1194–1197

    Google Scholar 

  8. Murashige T (1974) Plant propagation through tissue cultures. Ann Rev Plant Physiol 25: 135–166

    Google Scholar 

  9. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  10. Ochatt SJ, Cocking EC, Power JB (1987) Isolation, culture and plant regeneration of colt cherry (Prunus avium × pseudocerasus) protoplasts. Plant Sci 50: 139–143

    Google Scholar 

  11. Ramming DW (1985) In ovulo embryo culture of early-maturing Prunus Hort Sci 20: 419–420

    Google Scholar 

  12. Rubos AC, Pryke JA (1984) Morphogenesis in embryonic tissue cultures of apple. J Hort Sci 59: 469–475

    Google Scholar 

  13. Seirlis F, Mouras A, Salesses G (1979) Tentatives de culture in vitro d'anthères et de fragments d'organes ches les Prunus. Ann Amélior Plantes 29: 145–161

    Google Scholar 

  14. Van Nieuwkerk JP, Zimmerman RH, Fordham I (1986) Thidiazuron stimulation of apple shoots proliferation in vitro. Hort Sci 21: 516–518

    Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and permissions

About this article

Cite this article

Mante, S., Scorza, R. & Cordts, J.M. Plant regeneration from cotyledons of Prunus persica, Prunus domestica, and Prunus cerasus . Plant Cell Tiss Organ Cult 19, 1–11 (1989).

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI:

Key words