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Isolation and culture of cotton ovule epidermal protoplasts (prefiber cells) and analysis of the regenerated wall

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Abstract

Culture protocols were developed and characterization of the regenerated cell walls was performed for protoplasts of cotton (Gossypium hirsutum L., L., var. Acala SJ-2) ovule epidermal cells. This work was undertaken in order to extend studies concerning nutritional effects and regulation of nucleotide sugar incorporation into β-1,3- and β-1,4-glucan components of cotton fiber cell walls. Protein and carbohydrate polymers and recovered from the culture medium. Analysis of a cellular fraction indicated that the majority of 14C incorporated from [14C] glucose was present in the hot-water-soluble fraction of the cells. The majority of label incorporated into cell wall material could be solubilized with acetic-nitric reagent, indicative of noncellulosic material, and characterized as β-1,3-linked glucans. Only 5 to 15% of the regenerated cell wall could be characterized as β-1,4-linked glucose indicative of cellulose.

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Authors and Affiliations

  1. Department of Botany and Plant Sciences', University of California, 92521, Riverside, CA, USA

    Jean H. Gould, R. L. Palmer & W. M. Dugger

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  1. Jean H. Gould
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  2. R. L. Palmer
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  3. W. M. Dugger
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Gould, J.H., Palmer, R.L. & Dugger, W.M. Isolation and culture of cotton ovule epidermal protoplasts (prefiber cells) and analysis of the regenerated wall. Plant Cell Tiss Organ Cult 6, 47–59 (1986). https://doi.org/10.1007/BF00037758

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  • DOI: https://doi.org/10.1007/BF00037758

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