Abstract
Cell suspension cultures of Carica candamarcensis derived from hypocotyl calli were tested concerning their in vitro embryogenic capacity to improve asexual propagation rates in this species. Somatic embryos developed in culture from cells in suspension or from microcalli. Responses were affected by nutrient media and phytohormones used. Best results were obtained by growing the cells in suspension in Nitsch and Nitsch medium containing naphthaleneacetic acid and then plating them upon the same medium containing benzyladenine, or combinations of both hormones.
References
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158
Hanson MR (1984) Manipulation of single cells and protoplasts of plants: Experimental protocols, University of Virginia, Dept. Biology, Charlottesville VA 22903 USA.
Hermosilla J (1984) Papaína activa en Carica candamarcensis Proc. V Reunión Nacional de Botánica, La Serena, Chile.
Jordán M, Cortés I, Montenegro G (1983) Regeneration of plantlets by embryogenesis from callus cultures of Carica candamarcensis. Plant Sci Lett 28:321–326
Litz RE, Conover RA (1979) Development of systems for obtaining parasexual Carica hybrids. Proc Fla State Hort Soc 92:281–283.
Litz RE, Conover RA (1980) Somatic embryogenesis in cell cultures of Carica stipulata. Hort-Science 15:733–735.
Litz RE, Conover RA (1983) High-frequency somatic embryogenesis from Carica suspension cultures. Ann Bot 51:683–686
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87
Uphof JCTh (1968) Dictionary of economic plants. Verlag von J Cramer, Lehre, pp 591.
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Jordan, M. Somatic embryogenesis from cell suspension cultures in Carica candamarcensis . Plant Cell Tiss Organ Cult 7, 257–261 (1986). https://doi.org/10.1007/BF00037742
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DOI: https://doi.org/10.1007/BF00037742