Skip to main content
Log in

Somatic embryogenesis from cell suspension cultures in Carica candamarcensis

  • Short Communication
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Cell suspension cultures of Carica candamarcensis derived from hypocotyl calli were tested concerning their in vitro embryogenic capacity to improve asexual propagation rates in this species. Somatic embryos developed in culture from cells in suspension or from microcalli. Responses were affected by nutrient media and phytohormones used. Best results were obtained by growing the cells in suspension in Nitsch and Nitsch medium containing naphthaleneacetic acid and then plating them upon the same medium containing benzyladenine, or combinations of both hormones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158

    Google Scholar 

  2. Hanson MR (1984) Manipulation of single cells and protoplasts of plants: Experimental protocols, University of Virginia, Dept. Biology, Charlottesville VA 22903 USA.

    Google Scholar 

  3. Hermosilla J (1984) Papaína activa en Carica candamarcensis Proc. V Reunión Nacional de Botánica, La Serena, Chile.

  4. Jordán M, Cortés I, Montenegro G (1983) Regeneration of plantlets by embryogenesis from callus cultures of Carica candamarcensis. Plant Sci Lett 28:321–326

    Google Scholar 

  5. Litz RE, Conover RA (1979) Development of systems for obtaining parasexual Carica hybrids. Proc Fla State Hort Soc 92:281–283.

    Google Scholar 

  6. Litz RE, Conover RA (1980) Somatic embryogenesis in cell cultures of Carica stipulata. Hort-Science 15:733–735.

    Google Scholar 

  7. Litz RE, Conover RA (1983) High-frequency somatic embryogenesis from Carica suspension cultures. Ann Bot 51:683–686

    Google Scholar 

  8. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87

    Google Scholar 

  9. Uphof JCTh (1968) Dictionary of economic plants. Verlag von J Cramer, Lehre, pp 591.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jordan, M. Somatic embryogenesis from cell suspension cultures in Carica candamarcensis . Plant Cell Tiss Organ Cult 7, 257–261 (1986). https://doi.org/10.1007/BF00037742

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00037742

Key words

Navigation