Abstract
Growth and oxygen uptake of potato callus is faster in an oxygen-enriched atmosphere (70% oxygen, v/v; “oxygen-callus”) than in air (20% oxygen, v/v; “air-callus”). Especially the non-mitochondrial, so-called ‘residual respiration’ is increased in “oxygen-callus”. The capacities of the mitochondrial respiratory pathways (cytochrome pathway, Vcyt and alternative pathway, Valt) are also higher in this callus. In both callus types only a small part of the alternative pathway capacity is used in uninhibited respiration. The lower oxygen uptake of “air-callus” at normal air oxygen pressures is partially due to diffusional impedance. Measurement of the respiratory parameters of “air-callus” in oxygen-saturated medium leads to higher values than measurement in air-saturated medium, although these values are still lower than those of “oxygen-callus”.
ATP-production was calculated from the oxygen-uptake data and compared with the dry weight production of the callus to give values of 10.0 and 10.8 g dry weight produced.-mol ATP-1, for “air-callus” and “oxygen-callus” respectively. As no harmful side-effects are observed, cultivation of callus under elevated oxygen pressures may be useful, when rapid callus-growth is necessary.
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Abbreviations
- AA:
-
antimycin A; A;
- BHAM:
-
benzohydroxamate
- DW:
-
dry weight
- FW:
-
fresh weight
- 8-OHQ:
-
8-hydroxyquinolin
- RC:
-
respiratory control
- SHAM:
-
salicylhydroxamate
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van der Plas, L.H.W., Wagner, M.J. Effect of oxygen on growth and respiration of potato tuber callus. Plant Cell Tiss Organ Cult 7, 217–225 (1986). https://doi.org/10.1007/BF00037738
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DOI: https://doi.org/10.1007/BF00037738