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Purine glucosylating activity in cell suspension cultures ofSolanum tuberosum L.

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Abstract

Cell suspension cultures ofSolanum tuberosum L. cv. Adretta were established from leaf-derived calluses. In the search for purine glucosylating activity, the metabolism of 6-benzylaminopurine was studied. The main metabolite of BA was isolated and identified as 6-benzylaminopurine 7-β-d-glucopyranoside indicating the occurrence of purine glucosylating activity.

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Abbreviations

BA:

6-Benzylaminopurine

[3G]BA:

BA 3-β-d-glucopyranoside

[7G]BA:

BA 7-β-d-glucopyranoside

[9G]BA:

BA 9-β-d-glucopyranoside

RA:

Radioactivity

R T :

Retention Time

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Authors and Affiliations

  1. Institute of Plant Biochemistry, P.O.B. 250, D-06018, Halle (Saale), Germany

    D. Gottstein & W. Schliemann

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  1. D. Gottstein
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  2. W. Schliemann
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Gottstein, D., Schliemann, W. Purine glucosylating activity in cell suspension cultures ofSolanum tuberosum L.. Plant Cell Tiss Organ Cult 36, 265–268 (1994). https://doi.org/10.1007/BF00037730

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  • DOI: https://doi.org/10.1007/BF00037730

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