Plant Cell, Tissue and Organ Culture

, Volume 36, Issue 2, pp 259–264 | Cite as

Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.)

  • S. Rueb
  • M. Leneman
  • R. A. Schilperoort
  • L. A. M. Hensgens
Research Note


To obtain a reproducible efficient procedure for regeneration of rice plants through somatic embryogenesis from callus four published methods of callus induction and regeneration were compared. Callus was initiated from mature embryos of the Japonica cultivar Taipei 309 of rice (Oryza sativa L.). The number, mass and morphology of the callus formed on the scutellum were dependent on the medium used. A limited humidity and an optimal aeration of the culture vessels enhanced the frequency of embryogenesis and plant regeneration. A method described by Poonsapaya et al. (1989) was found to be the most efficient and was slightly modified. As a result 98% of the T309 embryos formed callus, of which 63% regenerated into plants. Each callus yielded an average of 6 plants. Plant morphology, fertility and seed set of the regenerants were found to be normal.

Key words

monocotyledons Oryza sativa L. plant regeneration rice somatic embryogenesis transformation 



2,4-dichlorophenoxyacetic acid


3-indole-acetic acid




standard error of mean


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • S. Rueb
    • 1
  • M. Leneman
    • 1
  • R. A. Schilperoort
    • 1
  • L. A. M. Hensgens
    • 1
  1. 1.Institute of Molecular Plant SciencesLeiden UniversityLeidenThe Netherlands

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