Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 36, Issue 2, pp 259–264 | Cite as

Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.)

  • S. Rueb
  • M. Leneman
  • R. A. Schilperoort
  • L. A. M. Hensgens
Research Note

Abstract

To obtain a reproducible efficient procedure for regeneration of rice plants through somatic embryogenesis from callus four published methods of callus induction and regeneration were compared. Callus was initiated from mature embryos of the Japonica cultivar Taipei 309 of rice (Oryza sativa L.). The number, mass and morphology of the callus formed on the scutellum were dependent on the medium used. A limited humidity and an optimal aeration of the culture vessels enhanced the frequency of embryogenesis and plant regeneration. A method described by Poonsapaya et al. (1989) was found to be the most efficient and was slightly modified. As a result 98% of the T309 embryos formed callus, of which 63% regenerated into plants. Each callus yielded an average of 6 plants. Plant morphology, fertility and seed set of the regenerants were found to be normal.

Key words

monocotyledons Oryza sativa L. plant regeneration rice somatic embryogenesis transformation 

Abbreviations

2,4-d

2,4-dichlorophenoxyacetic acid

IAA

3-indole-acetic acid

BA

6-benzyladeninepurine

S.E.M.

standard error of mean

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adkins SW, Shiraishi T & McComb JA (1990) Rice callus physiology—Identification of volatile emissions and their effects on culture growth. Physiol. Plant. 78: 526–531Google Scholar
  2. Chen T-H, Lam L & Chen S-C (1985) Somatic embryogenesis and plant regeneration from cultured young inflorescences ofOryza sativa L. (rice). Plant Cell Tiss. Org. Cult. 4: 51–54Google Scholar
  3. Genovesi AD & Magill CW (1982) Embryogenesis in callus derived from rice microspores. Plant Cell Rep. 1: 257–260Google Scholar
  4. Grimes HD & Hodges TK (1990) The inorganic NO3-:NH4+ ratio influences plant regeneration and auxin sensitivity in primary callus derived from immature embryos of Indica rice (Oryza sativa L.). J. Plant Physiol. 136: 362–367Google Scholar
  5. Heyser JW, Dykes TA, DeMott KJ & Nabors MW (1983) High frequency long term regeneration of rice from callus cultures. Plant Sci. Lett. 29: 175–182Google Scholar
  6. Li Z & Murai N (1990) Efficient plant regeneration from rice protoplasts in general medium. Plant Cell Rep. 9: 216–220Google Scholar
  7. Masuda K, Kudo-Shiratori A & Inoue M (1989) Callus transformation and plant regeneration from rice protoplasts purified by density gradient centrifugation. Plant Science 62: 237–246Google Scholar
  8. Morrish F, Vasil V & Vasil IK (1987) Developmental morphogenesis and genetic manipulation in tissue and cell cultures of the gramineae. Advances in Genetics 24: 431–499Google Scholar
  9. Poonsapava P, Nabors MW, Wright K & Vajrabhaya M (1989) A comparison of methods for callus culture and plant regeneration of RD25 rice (Oryza sativa L.) in two laboratories. Plant Cell Tiss. Org. Cult. 16: 175–186Google Scholar
  10. Potrykus I (1990) Gene transfer to cereals: an assessment. Bio/Technology 8: 535–542Google Scholar
  11. Roest S & Gilissen LJW (1989) Plant regeneration from protoplasts: a literature review. Acta Bot. Neerl. 38: 1–23Google Scholar
  12. Stickler MB (1991) Direct somatic embryogenesis and fertile plants from rice root cultures. J. Plant. Physiol. 138: 577–580Google Scholar
  13. Wernicke W, Brettell R, Wakizura T & Potrykus I (1981) Adventitious embryoid and root formation from rice leaves. Z. Pflanzenphysiol. 103: 361–365Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • S. Rueb
    • 1
  • M. Leneman
    • 1
  • R. A. Schilperoort
    • 1
  • L. A. M. Hensgens
    • 1
  1. 1.Institute of Molecular Plant SciencesLeiden UniversityLeidenThe Netherlands

Personalised recommendations