Abstract
The effect of different explant sources, growth regulators and coconut water concentrations and also light radiation on tissue cultures ofPassiflora edulis var.flavicarpa was evaluated.
In addition, plant regeneration was obtained from a diversity ofPassiflora germplasm. For shoot morphogenesis either cotyledonary, hypocotyledonary or leaf tissues were used to obtain cultures that formed shoots when placed on Murashige & Skoog basal medium containing 6-benzylaminopurine and coconut water. Direct organogenesis was observed.
Apex cultures have been also achieved fromP. edulis var.flavicarpa, P. mollissima, P. giberti, P. maliformis andP. amethystina. Excised explants 5 mm length were placed on half-strength basal medium, free of hormones. Whole plants were recovered.
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Abbreviations
- BA:
-
6-benzylaminopurine
- CW:
-
coconut water
- MS:
-
Murashige & Skoog (1962) medium
- NAA:
-
1-naphthaleneacetic acid
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Dornelas, M.C., Carneiro Vieira, M.L. Tissue culture studies on species ofPassiflora . Plant Cell Tiss Organ Cult 36, 211–217 (1994). https://doi.org/10.1007/BF00037722
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DOI: https://doi.org/10.1007/BF00037722