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Effect of medlium acidification on filamentous fungi, yeasts and bacterial contaminats inDelphinium tissue cultures

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Abstract

The persistence of accidentally introduced bacterial contaminants inDelphinium tissue cultures can be prevented by acidification of the tissue culture medium. Using this preservation method the contamination rate ofDelphinium cultures could be reduced by more than 50%.

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Abbreviations

MS:

Murashige & Skoog's (1962) medium

BA:

6-benzyladenine

cfu:

colony forming units

References

  • Bishop O N (1966), Statistics for Biology 2nd ed. (pp 117–121) Longman, London

    Google Scholar 

  • Bott JC (1980) Tissue culture ofDelphinium; Prelimnary experiments withDelphinium elatum and University Hybrids. Plantsman 2: 169–171

    Google Scholar 

  • Boxus Ph & Terzi J-M (1987) Big losses due to bacterial contamination can be avoided in mass propagation scheme. Acta Hort. 212: 91–93

    Google Scholar 

  • Boxus Ph & Terzi J-M (1987) Control of accidental contamination during mass propagation. Acta Hort. 225: 189–191

    Google Scholar 

  • Cooke DL, Waites WM & Leifert C (1992) Effects ofAgrobacterium tumefaciens, Erwinia carotovora, Pseudomonas syringae andXanthomonas campestris on plant tissue cultures ofAster, Cheiranthus, Delphinium, Iris andRosa; disease development in vivo as a result of latent infection in vitro. Journal of Plant Diseases and Protection 99: 469–481

    Google Scholar 

  • Danby S, Berger F, Howitt DJ, Wilson AR, Dawson S & Leifert C (1993) Fungal contaminants ofPrimula, Coffea, Musa andIris tissue cultures. Plant Growth Reg. (in press)

  • Kunneman BPAM & Faaij-Groenen (1988) Elimination of bacterial contaminants: A matter of detection and transplanting procedures. Acta Hort. 225: 183–188

    Google Scholar 

  • Leifert C & Waites WM (1992) Bacterial growth in plant tissue culture media. J. Appl. Bacteriol. 72: 460–466

    Google Scholar 

  • Leifert C & Waites WM (1993) Dealing with contaminants in plant tissue and cell culture; hazard analysis and critical control points. Plant Growth Reg. (in press)

  • Leifert C, Waites WM & Nicholas JR (1989a) Bacterial contaminants of micropropagated plant cultures. J. Appl. Bacteriol. 67: 353–361

    Google Scholar 

  • Leifert C, Waites WM, Camotta H & Nicholas JR (1989b)Lactobacillus plantarum; a deleterious contaminant of plant tissue cultures. J. Appl. Bacteriol. 67: 363–370

    Google Scholar 

  • Leifert C, Nicholas JR & Waites WM (1990) Yeast contaminants of micropropagated plant cultures. J. Appl. Bacteriol. 69: 471–476

    Google Scholar 

  • Leifert C, Ritchie J & Waites WM (1991a) Contaminants of plant tissue and cell cultures. World J. Microbiol. Biotechnol. 7: 452–469

    Google Scholar 

  • Leifert C, Camotta H, Wright SM, Waites B, Cheyne VA & Waites WM (1991b) Elimination ofLactobacillus plantarum, Corynebacterium spp.,Staphylococcus saprophyticus andPseudomonas paucimobilis from micropropagatedHemerocallis, Choisya andDelphinium using antibiotics. J. Appl. Bacteriol. 71: 307–330

    Google Scholar 

  • Leifert C, Camotta H & Waites WM (1992a) Effect of combinations of antibiotics on micropropagationClematis, Delphinium, Hosta, Iris andPhotinia. Plant Cell Tis. Org. Cult. 29: 153–160

    Google Scholar 

  • Leifert C, Pryce S, Lumsden PJ & Waites WM (1992b) Effect of medium acidity on growth and rooting of different plant species growing in vitro. Plant Cell Tis. Org. Cult. 30: 171–179

    Google Scholar 

  • Leifert C, Morris C & Waites WM (1993) Microbial populations on in vivo and in vitro plants. CRC Crit. Rev. Plant Sci. (in press)

  • Long RD, Curtin TF & Cassells AC (1988) An investigation of the effects of bacterial contaminants on potato nodal cultures. Acta Hort. 225: 83–91

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Pryce S, Lumsden PG, Berger F & Leifert C (1993) Effect of plant density and macronutrient nutrition onDelphinium shoot cultures. J. Hort. Sci. 68 (in press)

  • Schaad NW (1980) Initial identification of common genera. In: Schaad NW (Ed) Identification of Plant Pathogenic Bacteria (pp 1–11). American Phytopathological Society, St Paul

    Google Scholar 

  • Singha S, Bissonnette GK & Double ML (1987) Methods for sterilising instruments contaminated withBacillus sp. from plant tissue cultures. Hort. Sci. 22: 659

    Google Scholar 

  • Trick I & Lingens F (1985) Aerobic spore-forming bacteria as detrimental infectants in plant tissue cultures. Appl. Microbiol. Biotechnol. 21: 245–249

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Plant and Soil Science, University of Aberdeen, Cruickshank Building, AB9 2UD, Aberdeen, Scotland, UK

    C. Leifert

  2. Neo Plants Ltd., PR4 1HU, Freckleton, Lancashire, UK

    Barbara Waites, Julia W. Keetley, Sandra M. Wright & J. R. Nicholas

  3. Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, Leics, UK

    W. M. Waites

Authors
  1. C. Leifert
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  2. Barbara Waites
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  3. Julia W. Keetley
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  4. Sandra M. Wright
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  5. J. R. Nicholas
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  6. W. M. Waites
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Leifert, C., Waites, B., Keetley, J.W. et al. Effect of medlium acidification on filamentous fungi, yeasts and bacterial contaminats inDelphinium tissue cultures. Plant Cell Tiss Organ Cult 36, 149–155 (1994). https://doi.org/10.1007/BF00037713

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  • DOI: https://doi.org/10.1007/BF00037713

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