Skip to main content

Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb

Abstract

In vitro clonal multiplication of safed musli (Chlorophytum borivilianum Sant. et. Fernand.), a rare Indian medicinal herb, has been achieved on Murashige and Skoog's (MS) medium supplemented with 22.2 μM benzyladenine using young shoot bases as explants. Shoots multiplied at a rate of four-fold every 3 weeks. All shoots rooted when transferred to MS medium with 3/4-strength inorganic and organic constituents and 9.8 μM indolebutyric acid and 67% of the micropropagated plants were successfully established in pots. Such plants produced normal fasciculated storage roots as in wild plants.

This is a preview of subscription content, access via your institution.

Abbreviations

BA:

6-benzyladenine

2,4-d :

2,4-dichlorophenoxyacetic acid

IAA:

indoleacetic acid

IBA:

indolebutyric acid

MS:

Murashige and Skoog (1962)

NAA:

α-naphthaleneacetic acid

References

  • Arrillaga MCB & Segura J (1987) Somatic embryogenesis from hypocotyl callus cultures of Digitalis obscura L. Plant Cell Rep. 6: 223–226

    Google Scholar 

  • Balachandran SM, Bhat SR & Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep. 8: 521–524

    Google Scholar 

  • Bhat SR, Kackar A & Chandel KPS (1992) Plant regeneration from callus cultures of Piper longum L. by organogenesis. Plant Cell Rep. 11: 525–528

    Google Scholar 

  • Bhojwani SS (1980) In vitro propagation of garlic by shoot proliferation. Scientia. Hort. 13: 47–52

    Google Scholar 

  • Jat RD & Bordia PC (1990) Propagation studies in safed musli (Chlorophytum spp.) In: Chaudhary BL, Aery NC & Katewa SS (Eds) Proceedings of National Symposium on Advances in Plant Sciences: Current Status & Emerging Challenges (pp 46) Department of Botany, Sukhadia University, Udaipur

    Google Scholar 

  • Kirtikar KR & Basu BD (1975) Liliaceae Chlorophytum. In: Kirtikar KR & Basu BD (Eds) Indian Medicinal Plants (pp 2508–2509) Bishen Singh Mahendra Pal Singh, Dehradun, India

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Nayar MP & Sastry ARk (1988) Chlorophytum borivilianum. In: Nayar MP & Sastry ARk (Eds) Red Data Book of Indian Plants, Vol 2 (pp 142), Botanical Survey of India, Calcutta, India

    Google Scholar 

  • Pandey R, Chandel KPS & Rao SR (1992) In vitro propagation of Allium tuberosum Rottl. ex. Spreng. by shoot proliferation. Plant Cell Rep. 11: 375–378

    Google Scholar 

  • Vasil IK (1991) Rationale for the scale-up and automation of plant propagation. In: Vasil IK (Ed) Scale-up and Automation in Plant Propagation (pp 1–5), Academic Press Inc., London

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Purohit, S.D., Dave, A. & Kukda, G. Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb. Plant Cell Tiss Organ Cult 39, 93–96 (1994). https://doi.org/10.1007/BF00037596

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00037596

Key words