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Callus induction and plantlet regeneration in damiana (Turnera diffusa, Willd.)

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Abstract

Experiments were performed to determine optimal conditions for callus induction and plantlet regeneration from damiana (Turnera diffusa, Willd.) leaf explants. MS media [7] and B5 media [4] were used; both of them were supplemented with different combinations of 2,4-dichlorophenoxyacetic, acid (2,4-D) and 6-benzyl-adenine (BA). In all cases the samples were incubated under continous fluorescent light, 100 μmol.m-2.sec-1, at 25±2°C for 60 days. Highest callus percentage was obtained in MS media supplemented with 2,4-D 10−5 M plus BA 10-8M. Maximum percentage and multiple shoots and leaves were produced in MS media with BA 10-5M and BA 10-5M plus 2,4-D 10-7M. These explants were transferred for rooting to MS medium without plant growth regulators. Later, rooted plantlets were successfully established in soil.

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Authors and Affiliations

  1. Div. de Biología Experimental, Centro de Investigaciones Biológicas de Baja California Sur, Baja California Sur, Apdo. Postal 128, 23000, La Paz, México

    Antonio J. Díaz-Rondero & Lilia Alcaraz-Meléndez

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  1. Antonio J. Díaz-Rondero
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  2. Lilia Alcaraz-Meléndez
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Díaz-Rondero, A.J., Alcaraz-Meléndez, L. Callus induction and plantlet regeneration in damiana (Turnera diffusa, Willd.). Plant Cell Tiss Organ Cult 10, 39–45 (1987). https://doi.org/10.1007/BF00037495

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  • DOI: https://doi.org/10.1007/BF00037495

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